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pDIV296

Plasmid

#204687

Purpose

AMA1 plasmid with nat1 resistance marker

Depositor

Uffe Mortensen

Article

Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

Insert

nat1

Use

Fungal expression

Expression

Bacterial

Promoter

Aspergillus nidulans trpC promoter

Available Since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pBR BsmAIM

Plasmid

#206206

Purpose

Constitutive expression of BsmAIM methyltransferase

Depositor

Andrew Ellington

Article

Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

Insert

BsamAIM

Expression

Bacterial

Available Since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pET28sumo-hRig-I DCARDS

Plasmid

#210828

Purpose

protein expression in ecoli

Depositor

Anna Pyle

Article

Luo et al Cell. 2011 Oct 14;147(2):409-22. doi: 10

Insert

hRIG-I DCARDS (DDX58) with Caspase Recruitment domains deleted (RIGI Human)

Expression

Bacterial

Mutation

NA

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pSR1037

Plasmid

#208736

Purpose

Characterization plasmid for PSarA1 (sfgfp expressed from PSarA1 on the S. aureus pC194 replicon)

Depositor

Lauren Andrews

Article

Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

Insert

PSarA1

Use

Synthetic Biology

Expression

Bacterial

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pHS1950

Plasmid

#205983

Purpose

Ga0116245_10002768 T7/LacO-Cas6 + TwinStrep-Cas14 + Cas7 + His14-Cas5::pJ23119-DR-Fn spacer-DR

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Inserts

Ga0116245_10002768 candidate type VII proteins
Cas14
Cas5
CRISPR array

Tags

14xHis and TwinStrep-tag

Expression

Bacterial

Promoter

T7 (proteins), pJ23119 (crRNA)

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pHS1184

Plasmid

#205965

Purpose

NZ_CP025815 His14-TwinStrep-bdSUMO tagged DinG HNH protein (E. coli codon optimized) in pET45b(+)

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

DinG HNH

Tags

14xHis-TwinStrep-bdSUMO

Expression

Bacterial

Promoter

T7

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1 IntS8 AA 1-308

Plasmid

#196905

Purpose

Expresses His-tagged Drosophila IntS8 amino acids 1-308

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

Integrator subunit 8 (IntS8 Fly)

Tags

6xHis

Expression

Bacterial

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pmCellFree_KA2_ZIKV E

Plasmid

#145361

Purpose

Cell-free/mammalian expression vector for Zika virus Envelope protein with N-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV Envelope

Tags

8xHis and mCherry

Expression

Bacterial

Promoter

T7- CMV/CAG

Available Since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pDIV303

Plasmid

#204978

Purpose

AMA1 plasmid with Aspergillus optimized Mad7 and amdS selection marker

Depositor

Uffe Mortensen

Article

Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

Inserts

Mad7
amdS

Use

CRISPR; Fungal expression

Tags

SV40 NLS

Expression

Bacterial

Mutation

two silent mutations (nt177T>C, nt879C>T)

Promoter

Aspergillus nidulans tef1 promoter and Aspergillu…

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuet1_CylLL_CylM

Plasmid

#208759

Purpose

Expresses modified cytolysin L (mCylLL) in E. coli

Depositor

Wilfred van der Donk

Article

Luo et al Anal Chem. 2024 Jan 30;96(4):1767-1773.

Inserts

CylLL
CylLM

Tags

Hisx6 Tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuet1_CoiA1_CoiB

Plasmid

#208761

Purpose

Precursor peptide CoiA1 and synthase CoiB, for the co-expression of CoiA1 in E.coli.

Depositor

Wilfred van der Donk

Article

Luo et al Anal Chem. 2024 Jan 30;96(4):1767-1773.

Inserts

CoiA1
CoiB

Tags

Hisx6 Tag and SUMO

Expression

Bacterial

Mutation

Changed Ser 20 to Asn, Changed Arg 84 to Lys, bot…

Promoter

T7

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a MDHC_YEAST

Plasmid

#204232

Purpose

Bacterial expression of yeast cytosolic malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).

Depositor

Malate dehydrogenase CUREs Community (MCC)

Insert

MDHC_YEAST (MDH2 Budding Yeast)

Tags

6X His

Expression

Bacterial

Promoter

Lac

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pET-21(+)Tm9D8* TrpB

Plasmid

#207517

Purpose

Expression of Tm9D8* TrpB for the synthesis of 7-Aza-L-Tryptophan (7AW) from 7-Azaindole (7AI) and serine

Depositor

Thomas Huber

Article

Abdelkader et al ACS Sens. 2023 Oct 27. doi: 10.1021/acss

Insert

Tryptophan synthase β-subunit

Expression

Bacterial

Promoter

T7

Available Since

Oct. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pET21a-C2m2-SNAP

Plasmid

#208773

Purpose

To express C2m2-SNAP in bacteria

Depositor

Urte Neniskyte

Article

Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

Insert

C2m2-SNAP (Mfge8 Mouse)

Tags

SNAP-tag

Expression

Bacterial

Mutation

Mutated 24 and 45 lysine to asparagine in C2 doma…

Promoter

T7 promoter

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pQE60 R130A wgMDH

Plasmid

#204266

Purpose

Bacterial expression of R130A wgMDH with IPTG induction - Active site mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Tags

6X His

Expression

Bacterial

Mutation

R130A

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pMB1-A-TelR

Plasmid

#204979

Purpose

Level 1 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains

Depositor

Veronica Godoy-Carter

Article

Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

Insert

mRFP1

Use

Synthetic Biology

Expression

Bacterial

Mutation

Added replications elements for Acinetobacter spe…

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pAJT194

Plasmid

#202293

Purpose

Type IIS cloning plasmid for LPattB5 integrations w/ OriT

Depositor

Christopher Voigt

Article

Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

Insert

sfGFP

Expression

Bacterial

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pET19-MTdTwt

Plasmid

#207460

Purpose

Bacterial expression of Terminal deoxynucleotidyl transferase (TdT)

Depositor

Jay Keasling

Article

Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

Insert

MTdTwt (Dntt Mouse)

Tags

10xHis-MBP

Expression

Bacterial

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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PJEBAN3

Plasmid

#203682

Purpose

Expresses Yellow Fluorescent Protein (YFP) in Listeria monocytogenes

Depositor

Tine Rask Licht

Article

Andersen et al BMC Microbiol. 2006 Oct 3;6:86. doi: 10.

Insert

Yellow Fluorescent Protein (YFP)

Expression

Bacterial

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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PJEBAN4

Plasmid

#203683

Purpose

Expresses red fluorescent marker HcRed in Listeria monocytogenes

Depositor

Tine Rask Licht

Article

Andersen et al BMC Microbiol. 2006 Oct 3;6:86. doi: 10.

Insert

HcRed

Expression

Bacterial

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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