33,526 results
-
Plasmid#221709PurposeEmpty Cas12a vector for multiplex gene editing in fungiDepositorTypeEmpty backboneUseCRISPR; AspergillusExpressionBacterialAvailable sinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only
-
pAK004
Plasmid#233641PurposeFor bacterial expression of eSpCas9(1.1) c-terminally tagged with VitelloTagA.DepositorInserteSpCas9(1.1) with c-terminal VitelloTagA
Tags6-HisExpressionBacterialAvailable sinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
p344_DG
Plasmid#234674PurposeGolden gate domesticated pET28b with His6-3CDepositorTypeEmpty backboneTagsHis6-3CExpressionBacterialMutationNoAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p356_DG
Plasmid#234699PurposeGolden gate domesticated pET28b for scarless cloning with His10-MBP-3CDepositorTypeEmpty backboneTagsHis10-MBP-3CExpressionBacterialMutationNoAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p353_DG
Plasmid#234696PurposeGolden gate domesticated pET28b for scarless cloning with His10-3CDepositorTypeEmpty backboneTagsHis10-3CExpressionBacterialMutationNoAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
NP-11-64-1
Plasmid#234359PurposeExpressing rubisco and prk in ΔrpiDepositorInsertR.rubrum rubisco
UseSynthetic BiologyExpressionBacterialPromoterPtacAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_N-myc
Plasmid#234045PurposeBacterial expression of N-terminally 6His-tagged N-myc; includes TEV cleavage siteDepositorAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_MAX
Plasmid#234044PurposeBacterial expression of N-terminally 6His-tagged MAX; includes TEV cleavage siteDepositorAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pIA123
Plasmid#234038PurposeE. coli-C. difficile shuttle vector for CRISPR editing in C. difficile. Pxyl::Cas9-opt Pgdh::sgRNA-cdr_0985. PstI site to add DNA for homology directed repair. MscI and NotI for replacement of sgRNA.DepositorInsertsCas9
sgRNA
UseCRISPRExpressionBacterialPromoterPgdh and PxylAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNCS-mGold2t
Plasmid#231766PurposeExpression of mGold2t in bacterial cellsDepositorInsertmGold2t
ExpressionBacterialMutationmGold2t is mGold with V1A;V22I;H77N;Q80R;K101E;D1…PromoterSynthetic promoterAvailable sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCV019-catP
Plasmid#232825PurposeRandom transposon mutagenesis vector for Bacteroidales species - chloramphenicolDepositorInsertcatP
ExpressionBacterialAvailable sinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
KPC2-pNic-Bsa4
Plasmid#231082Purposeexpresses Klebsiella pneumoniae carbapenemase-2 (KPC-2) under T7 with His tagDepositorAvailable sinceFeb. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pZP06
Plasmid#232320PurposepZP05 was constructed by cloning the oriFn-Pfdx-E-repA fragment from pCWU6 into the smaller suicide plasmid pCM-galK, creating a conditional plasmid with replication controlled by Pfdx-E.DepositorInsertPfdx-E
ExpressionBacterialAvailable sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEND-227_Cacnes_mCerulean
Plasmid#225614PurposeReplicative vector for blue fluorescent reporter protein mCerulean strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+mCerulean.DepositorInsertmCerulean
UseSynthetic BiologyTags6xHisExpressionBacterialMutationCodon optimized for Cutibacterium acnesAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
PGL4.11-COL1A1mSMAD
Plasmid#230917PurposeTested the COL1A1 promoter activity after abolition of the proximal SMAD binding sites in the promoter.DepositorInsertHuman COL1A1 promoter with mutant SMAD binding sequence (COL1A1 Human)
UseLuciferaseTagsLuciferase-luc2pExpressionBacterial and MammalianMutationCOL1A1 promoter with mutant potential SMAD bindin…PromoterCOL1A1Available sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCN38-kimA-yfp
Plasmid#227635Purposec-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding kimA riboswitch. Plasmid confers ampicillin resistance to E. coli and chloramphenicol resistance to S. aureus.DepositorInsertkimA-yfp
ExpressionBacterialAvailable sinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGMβ1
Plasmid#216202PurposeChromosomal gene manipulation (gene insertion, conversion, deletion) of dairy used Lactobacillus bulgaricus, a difficult gene to manipulate, is now possible by conjugation using this pGMB1 plasmid.DepositorTypeEmpty backboneUseShuttle vector, conjugal plasmid, theta type rep…ExpressionBacterialPromoterlac promoter in pGEM-Teasy, Promoters in pAMbeta1…Available sinceJan. 21, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
PET-28a-YTH domain of YTHDF1
Plasmid#228775PurposeProtein expression plasmid for YTH domain of YTHDF1 in E.Coli.DepositorAvailable sinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
PET-28a-YTH domain of YTHDF2
Plasmid#228776PurposeProtein expression plasmid for YTH domain of YTHDF2 in E.Coli.DepositorAvailable sinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET29-neoLux1.2
Plasmid#229952PurposeBacterial expression for de novo luciferase, neoLux1.2DepositorInsertneoLux1.2
TagsHis-tagExpressionBacterialPromoterT7Available sinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only