31,828 results
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Plasmid#154057PurposeLevel 0 Golden Gate vector, containing the ZmUbi promoter and 5' UTR with Level 0.5-compatible overhangs (Wheat construct)DepositorInsertPromoter and 5' UTR Ubiquitin (Zea mays)
UseSynthetic BiologyExpressionBacterialPromoterN/AAvailable SinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
B7-5
Plasmid#111493Purposetranslational initiation factor 1DepositorInserttranslational initiation factor 1
Tags6xHisExpressionBacterialAvailable SinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
SND1 (SND1A-c003)
Plasmid#98242PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertSND1A (SND1 Human)
TagsHis-6-TEVExpressionBacterialMutationpfam00567:TUDOR 677-799PromoterT7Available SinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMM585
Plasmid#68884PurposeIPTG-inducible NanoLuc expression, pNBU2 backbone, AmpRDepositorInsertsNanoLuc
LacI
ExpressionBacterialAvailable SinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-18
Plasmid#172763PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-18; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-18
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCARSF63
Plasmid#64695PurposeH6-TRX-Smt3-GB1-NpuDnaEN102-CBDDepositorTypeEmpty backboneTagsCBD, HisTag, NpuDnaE-N102, Smt3, and TRXExpressionBacterialPromoterT7Available SinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET3a-His-DJ1
Plasmid#51488PurposeInducible expression of human His-DJ1 in E.coliDepositorAvailable SinceMarch 31, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFP.R265
Plasmid#138167PurposeExpresses the split mCherry reporter interrupted by the SspDnaB M86 artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRAF-MutH
Plasmid#86609PurposeE. coli MutH expression plasmidDepositorInsertE. coli MutH
Tagshis-tag and ald-tagExpressionBacterialPromoterT7Available SinceFeb. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET32a-hEPR
Plasmid#199233PurposeBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.DepositorInsertProepiregulin preproprotein (EREG Human)
TagsTrxA-6xHis-S-tagExpressionBacterialPromoterT7Available SinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKB232
Plasmid#170019PurposeEncodes LacI-controlled expression of surface-displayed murine CRAMP and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
CRAMP
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialPromoterPtac and PvirKAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_TXN_Thioredoxin
Plasmid#109778PurposeProtein expression and purification of TXN_ThioredoxinDepositorInsertTXN_Thioredoxin (TXN Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
P-BT3332
Plasmid#188110PurposepBolux harboring the promoter region upstream of BT3332DepositorInsertThe B. thetaiotaomicron BT3332 promoter cloned into pBolux
ExpressionBacterialPromoterBT3332Available SinceJan. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-mEGFP
Plasmid#185013PurposeBacterial Expression of 6xHis Sirius-GFP Fusion ProteinDepositorInsertSirius-GFP
ExpressionBacterialAvailable SinceJune 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLAC
Plasmid#45072Purposelacritin-intein fusion plasmid coding for intact mature lacritin without signal peptideDepositorInsertLacritin (LACRT Human)
UseProtein expression vectorTagsInteinExpressionBacterialPromoterT7Available SinceSept. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPBC-LG6f
Plasmid#62808PurposepPBC-LG6f expresses PiggyBac inverted terminal repeat-flanked Lck-GCaMP6f under the control of the CAG promoter.DepositorInsertITR-CAG-Lck-GCaMP6f-ITR
ExpressionBacterial and MammalianPromoterCAGAvailable SinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_PTPN22_Y-phosphatase
Plasmid#110048PurposeProtein expression and purification of PTPN22_Y-phosphataseDepositorInsertPTPN22_Y-phosphatase (PTPN22 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTarget: Ptaq-RFP_PlacIq-GFP
Plasmid#192280PurposeRFP expressed under constitutive promoter Ptaq and GFP expressed under constitutive promoter PlaciqDepositorInsertsRFP
GFP
ExpressionBacterialPromoterPlacIq and PtaqAvailable SinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcNB2
Plasmid#89242PurposeResurfaced Cell-penetrating 5F7 nanobodyDepositorInsertpcNB2 (5F7)
ExpressionBacterialPromoterT7Available SinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMS20: pHelper(PmcCAST)_PmcPSP1_ΔTnsD
Plasmid#168153PurposeInducible expression of PmcCAST proteins (except TnsD) with crRNA targeting PmcPSP1.DepositorInsertsPmcCAST minimal CRISPR array (with spacer for PmcPSP1)
PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
PmcCAST Tns proteins (TnsAB and TnsC)
PmcTniQ
ExpressionBacterialAvailable SinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only