32,963 results
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Plasmid#173172PurposeExpresses SO_4387DepositorInsertSO_4387
ExpressionBacterialPromoterJ23107Available SinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTet-tns
Plasmid#170628PurposeExpression of CRISPR-associated transposasesDepositorInsertVchTnsABC
ExpressionBacterialAvailable SinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaM-6
Plasmid#172771PurposeBacterial expression of anti-mCherry nanobody LaM-6, with pelB leader and C-term free cysteine and 6xHIS tag.DepositorInsertLaM-6
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-18
Plasmid#172763PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-18; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-18
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-41-4GS-LaG-2
Plasmid#172766PurposeBacterial expression of dimerized anti-GFP nanobodies LaG-41 and LaG-2 (4GS linker)DepositorInsertLaG-41-4GS-LaG-2
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-11
Plasmid#172762PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-11; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-11
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pRSETA_ionRD
Plasmid#172924PurposeIonic strength FRET sensor with mCerulean3 and mCitrine for E. coli expression.DepositorInsertIonicStrengthSensorRD
TagsHisExpressionBacterialPromoterT7Available SinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSETA_crE6G2
Plasmid#172929PurposeCrowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 2 helices, and a 6AA GSG domainDepositorInsertCrowdingSensorE6G2
TagsHisExpressionBacterialMutationVal317Gly, Met316GlyPromoterT7Available SinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTU1-A-SP44-mVenus-I
Plasmid#163763PurposeExpresses Streptomyces codon-optimised mVenus-IDepositorInsertmVenus-I
UseSynthetic BiologyTags6xHisExpressionBacterialPromoterSP44Available SinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTU1-B-SP44s-GUS
Plasmid#172430PurposeExpresses Streptomyces codon-optimised beta-D-glucuronidaseDepositorInsertbeta-D-glucuronidase
UseSynthetic BiologyTagsNoneExpressionBacterialPromoterSP44sAvailable SinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAT207
Plasmid#134884PurposeBacterial expressionDepositorInsertmRFP1
UseSynthetic BiologyExpressionBacterialAvailable SinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRC1765
Plasmid#141346PurposeMini plasmid containing minimal sequences for amplification in bacteria and two BbvCI nicking sites for introduction of modified DNA through annealing and ligation (e.g. mismatch, DNA lesions, flap).DepositorTypeEmpty backboneExpressionBacterialAvailable SinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLG163
Plasmid#124365PurposeIPTG inducible system (gfpmut2 with Ppsank) for expression in B. subtilisDepositorAvailable SinceJuly 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDYU1B/mRuby3–α-tubulin
Plasmid#154302PurposeExpresses mRuby3–α-tubulin from actin15 promoter in Dictyostelium discoideumDepositorInsertalpha-tubuin
UseDictyostelium expressionTagsmRuby3ExpressionBacterialAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET11a_3xFlag-nsp14 (SARS-CoV-2)
Plasmid#169159PurposeTo express SARS-CoV-2 nsp14 in E. coliDepositorInsert3xFlag-nsp5CS[VATLQ]-nsp14 (ORF1ab SARS-CoV-2)
Tags3xFlag and nsp5CS[VATLQ]: predicted nsp5 protease…ExpressionBacterialMutationCodon optimised for E. coliPromoterT7Available SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSU315
Plasmid#169697PurposeTemplate plasmid for generating C-terminal hemagglutinin (1xHA) epitope tag fusions to bacterial genes by "Lambda red" recombineering.DepositorInsert1xHA epitope tag adjacent to FRT-flanked aph (KanR) gene
Tags1xHA tagExpressionBacterialAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSUB7
Plasmid#169698PurposeTemplate plasmid for generating C-terminal 6-His epitope tag fusions to bacterial genes by "Lambda red" recombineering.DepositorInsert6xHis epitope tag adjacent to FRT-flanked aph (KanR) gene
Tags6xHis tagExpressionBacterialAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGBK CUP1 mGrape3
Plasmid#170265PurposeYeast two-hybrid vector using a copper inducible CUP1 promoter, expressing mGrape3DepositorArticleInsertmomeric Grape 3
TagsGAL4 DNA Binding Domain and MycExpressionBacterial and YeastPromoterCUP1Available SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKB231
Plasmid#170018PurposeEncodes LacI-controlled expression of surface-displayed cecropin P1 and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
Cecropin P1
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialPromoterPtac and PvirKAvailable SinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only