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31,828 results

Showing: 8641 - 8660 of 31828 results

  8. htpG2-GFP

    Plasmid
    #109388
    Purpose
    GFP cassette under the control of the htpG2 sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.
    Depositor
    Tom Ellis
    Article
    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463
    Insert
    phtpG2-GFP
    Expression
    Bacterial
    Available Since
    May 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    109388-plasmid-map-sequence-id-210731

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  13. pACYC-T7-SpCas9(BspMI/SapI/BsaI_cassettes)-T7-gRNA1 (BPK1807)

    Plasmid
    #223065
    Purpose
    Dual pT7 entry plasmid for SpCas9(6AA) library; bacterial expression plasmid with type IIS RE cassettes around D1135/S1136, G1218/E1219, R1335/T1337 (precursor to MMW94). Expresses a gRNA.
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    entry vector for human codon opt. bacterial expr. plasmid for SpCas9(6AA_NNS) library, with gRNA targeting EGFP site 1
    Use
    CRISPR
    Tags
    NLS(SV40)-3xFLAG
    Expression
    Bacterial
    Mutation
    Three regions of SpCas9 encode type IIS restricti…
    Promoter
    Dual T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223065-plasmid-map-sequence-id-447614

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  18. pZ8-Prp

    Plasmid
    #74065
    Purpose
    pZ8-1 with the PprpD2 propionate-inducible promoter instead of Ptac, KanR
    Depositor
    Timothy Lu
    Article
    Cleto et al ACS Synth Biol. 2016 Feb 16.
    Insert
    Propionate inducible promoter
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    prp
    Available Since
    April 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    74065-plasmid-map-sequence-id-138071

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  19. 6CCR (GID4)

    Plasmid
    #119960
    Purpose
    Bacterial expression for structure determination
    Depositor
    Cheryl Arrowsmith
    Insert
    GID4 (C17orf39 Human)
    Tags
    6xHIS, thrombin,SUMO and TEV cleavage
    Expression
    Bacterial
    Mutation
    contains amino acids 116-300
    Promoter
    T7
    Available Since
    Jan. 8, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    119960-plasmid-map-sequence-id-232678

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Showing: 8641 - 8660 of 31828 results