30,239 results

1. pSB1C3 I0500 LasI

   Plasmid

   #107966

   Purpose

   Arabinose-inducible 3OC12HSL sender plasmid

   Depositor

   Jim Haseloff

   Article

   Boehm et al Nat Commun. 2018 Feb 22;9(1):776. doi: 1

   Insert

   lasI
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKNR5

   Plasmid

   #149466

   Purpose

   Broad-Host range expression vector, RK2 and pUC origin, NahR(AM) repressor, PSalTTC promoter, mRFP, Gentamicin Resistance Marker.

   Depositor

   Christopher Reisch

   Article

   Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

   Insert

   NahR(AM)-mRFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PSalTTC

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. BPV00554 pET21GG2-His(6)-BECN(248-450)

   Plasmid

   #136685

   Purpose

   Expresses the Beclin-1 ECD/BARA domain (a.a. 248-450) with a N-terminal hexa-Histidine tag

   Depositor

   Colin Garvie

   Article

   Ranaghan et al Biochemistry. 2017 Dec 26;56(51):6639-66

   Insert

   Beclin-1 ECD/BARA (BECN1 Human)
   

   Use

   Tags

   Hexa-Histadine

   Expression

   Bacterial

   Mutation

   DNA optimized for E. coli expression

   Promoter

   T7

   Available since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKDT17

   Plasmid

   #27503

   Depositor

   Peter Greenberg

   Article

   Pearson et al Proc Natl Acad Sci U S A. 1994 Jan 4. 91

   Insert

   LasR, LasB::LacZ fusion (lasR Pseudomonas aeruginosa)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 18, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pXD71Cas10.4RT4-Pct5.1-crRNA(cgr2)-RT(Δcgr12)

   Plasmid

   #191650

   Purpose

   E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(cgr2-targeting spacer) cgr1/cgr2-deleting repair template, used for cgr1/cgr2 deletion

   Depositor

   Emily Balskus

   Article

   Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

   Insert

   CymR
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGBK CUP1 mGrape3

   Plasmid

   #170265

   Purpose

   Yeast two-hybrid vector using a copper inducible CUP1 promoter, expressing mGrape3

   Depositor

   Bruce Hamilton

   Article

   Hernandez et al bioRxiv

   Insert

   momeric Grape 3
   

   Use

   Tags

   GAL4 DNA Binding Domain and Myc

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   CUP1

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLacI241-Wasabi

   Plasmid

   #86102

   Purpose

   mWasabi reporter gene gated by temperature-sensitive LacI A241T

   Depositor

   Mikhail Shapiro

   Article

   Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

   Inserts

   LacIts
   mWasabi
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   A241T

   Promoter

   T7A1O4/O3 and pLacI

   Available since

   Feb. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pZH516

   Plasmid

   #102669

   Purpose

   Constitutive expression of GFPmut2; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

   Depositor

   Zach Hensel

   Article

   Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

   Insert

   GFPmut2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pQY

   Plasmid

   #48098

   Purpose

   Cumate-inducible expression of SYFP2-tagged fusion protein

   Depositor

   Julia Vorholt

   Article

   Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

   Type

   Empty backbone

   Use

   Tags

   3xFLAG and SYFP2

   Expression

   Bacterial

   Mutation

   Promoter

   PQ5

   Available since

   Jan. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. FtsZ-Dendra2

    Plasmid

    #92038

    Purpose

    Express FtsZ-Dendra2

    Depositor

    Ji Yu

    Article

    Niu et al Biophys J. 2008 Aug;95(4):2009-16. doi:

    Insert

    FtsZ
    

    Use

    Tags

    Dendra2

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDZ617 pKAN 12xPP7 V4

    Plasmid

    #72237

    Purpose

    Floxed Kanamycin (G418) selection maker; 3' tagging vector used to add 12 repetitive PP7 version 4 RNA stem-loops to gene of interest

    Depositor

    Daniel Zenklusen

    Article

    Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

    Insert

    12xPP7 V4
    

    Use

    Cre/Lox

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pE4c

    Plasmid

    #17464

    Purpose

    modified pENTR vector for C-terminal GST tag fusion with your gene of interest

    Depositor

    Giovanna Benvenuto

    Article

    Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.

    Type

    Empty backbone

    Use

    Modified gateway entry vector

    Tags

    GST

    Expression

    Bacterial, Insect, Mammalia…

    Mutation

    Promoter

    Available since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. pETM33_TNKS_TANK-peptide-bind

    Plasmid

    #178489

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    TNKS_TANK-peptide-bind (TNKS Human, Synthetic)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7/LacO

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYTK077

    Plasmid

    #65184

    Purpose

    Encodes KanamycinR as a Type 6 part to be used in the Dueber YTK system

    Depositor

    John Dueber

    Article

    Lee et al ACS Synth Biol. 2015 Apr 14.

    Insert

    KanamycinR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. csr-1_son-1_vsfGFP9

    Plasmid

    #191747

    Purpose

    Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

    Depositor

    Jay C Dunlap

    Article

    Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

    Insert

    NCU04288 (NCU04288 Neurospora crassa)
    

    Use

    Tags

    NCU04502 promoter and vsfGFP-9

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pETDuet-T7-Ct-YhjH

    Plasmid

    #79167

    Purpose

    Dual expression of Ct biosensor and YhjH under individual T7 promoters

    Depositor

    Ming Hammond

    Article

    Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

    Inserts

    yhjH
    T7-Spinach2-Ct
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJ4M_hnRNPA2_FL_D290V

    Plasmid

    #139110

    Purpose

    Express FL hnRNPA2 D290V with a C-terminal MBP tag

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al Nucleic Acids Res. 2020 Sep 1. pii: 5900

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D290V

    Promoter

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pETconNK-TEM1(S70A,D179G)

    Plasmid

    #81166

    Purpose

    pETconNK with TEM1(S70A,D179G) beta-lactamase

    Depositor

    Timothy Whitehead

    Article

    Klesmith et al Proc Natl Acad Sci U S A. 2017 Feb 14. p

    Insert

    TEM-1 beta-lactamase
    

    Use

    Tags

    c-myc

    Expression

    Bacterial and Yeast

    Mutation

    Deleted the sec transport tag. Plasmid encodes H2…

    Promoter

    GAL1 and gal1

    Available since

    Feb. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. SRpHi3

    Plasmid

    #102543

    Purpose

    Bacterial expression of sepHluorin fused to the cell penetrating peptide TAT with a GST tag for purification

    Depositor

    Stefan Hell

    Article

    Richardson et al Nat Commun. 2017 Sep 18;8(1):577. doi: 1

    Insert

    Tat-sepHluorin
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. TAF1

    Plasmid

    #39118

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    TAF1 (TAF1 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only