30,249 results

1. C164

   Plasmid

   #141128

   Purpose

   Expresses sfYFP under lac promoter

   Depositor

   Matthew Bennett

   Article

   Alnahhas et al Nat Commun. 2020 Jul 21;11(1):3659. doi:

   Insert

   sfYFP
   

   Use

   Tags

   ssrA degradation tag

   Expression

   Bacterial

   Mutation

   Promoter

   engineered lac promoter

   Available since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a(cd226)

   Plasmid

   #177900

   Purpose

   Bacterial expression of his10-tagged human cd226 Intracellular Domain

   Depositor

   Ron Vale

   Article

   Hui et al Science. 2017 Mar 31;355(6332):1428-1433

   Insert

   CD226 (aa 288-336 only) (CD226 Human)
   

   Use

   Tags

   10xHis-Thrombin cut site-T7 tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTrc99S-ssDsbA-TTc-4xDQNAT

   Plasmid

   #128401

   Purpose

   Periplasmic expression of tetanus toxin fragment C with C terminal 4xDQNAT glycosylation sites in E. coli

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

   Insert

   Tetanus toxin fragment C domain
   

   Use

   Tags

   4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

   Expression

   Bacterial

   Mutation

   Promoter

   trc

   Available since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTrc99S-ssDsbA-TTlight-4xDQNAT

   Plasmid

   #128402

   Purpose

   Periplasmic expression of tetanus toxin light chain with C terminal 4xDQNAT glycosylation sites in E. coli

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

   Insert

   Tetanus toxin light chain domain
   

   Use

   Tags

   4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

   Expression

   Bacterial

   Mutation

   Inactivating E234A mutation

   Promoter

   trc

   Available since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQE30-TEV-pro-conA

   Plasmid

   #159527

   Purpose

   Expresses pro-concanavalin A, a lectin from Canavalia ensiformis, in E. coli. Includes N-terminal TEV recognition site. Native N-terminal sequence is reconstituted upon TEV cleavage.

   Depositor

   Joshua Mylne

   Article

   Nonis et al bioRxiv. 2020.

   Insert

   Canavalia ensiformis pro-concanavalin A
   

   Use

   Tags

   Six-Histidine tag and tobacco etch virus (TEV) pr…

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJ2076.2_cL

   Plasmid

   #140668

   Purpose

   cL control for toggle switch experiment

   Depositor

   Yolanda Schaerli

   Article

   Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

   Insert

   cL control for toggle switch experiment
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. HisMS2_VLP_FluA

   Plasmid

   #175955

   Purpose

   Generation of MS2 Virus-Like Particles packaged with the sequence for the Influenza A Matrix Protein [A/Illinois/20/2018(H1N1)]

   Depositor

   Paul Freemont

   Article

   Unruh et al medRxiv 2024.04.17.24305965

   Insert

   M gene
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 19, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pTXB1-hMeCP2

   Plasmid

   #48091

   Purpose

   Bacterial expression of Mxe intein/chitin binding domain tagged MeCP2-WT

   Depositor

   Huda Zoghbi

   Article

   Baker et al Cell. 2013 Feb 28;152(5):984-96. doi: 10

   Insert

   Methyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Mxe intein/chitin binding domain tagged

   Promoter

   Available since

   April 17, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pMN234

   Plasmid

   #32108

   Depositor

   Michael Niederweis

   Article

   Stephan et al Gene. 2004 Dec 8;343(1):181-90.

   Insert

   flpe
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   imyc

   Available since

   Nov. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pUC-UAS1B4-Leum

    Plasmid

    #44312

    Depositor

    Hal Alper

    Article

    Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

    Insert

    UAS1B4-Leum promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    UAS1B4-Leum

    Available since

    July 15, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pKP325

    Plasmid

    #159596

    Purpose

    Express E.coli TonB protein

    Depositor

    Kathleen Postle

    Article

    Larsen et al Mol Microbiol. 1999 Mar;31(6):1809-24. d

    Insert

    tonB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAPA3

    Plasmid

    #24777

    Depositor

    Tanya Parish

    Article

    Parish et al J Bacteriol. 2007 May . 189(10):3721-8.

    Type

    Empty backbone

    Use

    Integrating vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTriEX-Antenna-GDI Rac1

    Plasmid

    #83359

    Purpose

    Expression of Antenna-Rac1

    Depositor

    Klaus Hahn

    Article

    Hodgson et al Nat Chem Biol. 2016 Aug 8. doi: 10.1038/

    Insert

    Rac1 (RAC1 Human)
    

    Use

    Tags

    FRET Antenna (mCerulean-cpVenus) and His

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    CMV

    Available since

    March 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET11a_3xFlag-nsp14 (SARS-CoV-2)

    Plasmid

    #169159

    Purpose

    To express SARS-CoV-2 nsp14 in E. coli

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

    Insert

    3xFlag-nsp5CS[VATLQ]-nsp14 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    3xFlag and nsp5CS[VATLQ]: predicted nsp5 protease…

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T7

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJJH1260

    Plasmid

    #36913

    Depositor

    Jürgen Heinisch

    Article

    Bakota et al Mol Genet Genomics. 2012 Apr;287(4):313-

    Inserts

    ScURA3
    ScURA3
    

    Use

    Tags

    Expression

    Bacterial, Mammalian, and Y…

    Mutation

    Promoter

    Available since

    July 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pVRb01_syn20

    Plasmid

    #49704

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    sfgfp
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pecf01_syn20

    Available since

    Jan. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET30 GLTP

    Plasmid

    #170733

    Purpose

    The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

    Depositor

    Rhoderick Brown

    Article

    Malinina et al Nature. 2004 Aug 26;430(7003):1048-53. d

    Insert

    GLTP (GLTP Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pZac2.1 gfaABC1D-3xHA-SAPAP3

    Plasmid

    #190200

    Purpose

    Expresses SAPAP3 protein fused with an HA tag at the N-terminus in astrocytes

    Depositor

    Baljit Khakh

    Article

    Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

    Insert

    3xHA-SAPAP3 (Dlgap3 Mouse)
    

    Use

    AAV

    Tags

    HA

    Expression

    Bacterial

    Mutation

    Promoter

    gfaABC1D

    Available since

    April 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET His6 Sumo TEV co-transformation cloning vector (13K-S)

    Plasmid

    #48321

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    His6 and Sumo

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET29b-Ava3858-3855

    Plasmid

    #45746

    Depositor

    Emily Balskus, Christopher T Walsh

    Article

    Balskus et al Science. 2010 Sep 24;329(5999):1653-6. d

    Insert

    Ava3858-3855
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only