32,457 results
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Plasmid#81012PurposeExpression of Zdk1 anchored to mitochondriaDepositorInsertZdk3
Tags6x His tag, FLAG, NTOM20, and mVenusExpressionBacterial, Insect, and Mamm…Available SinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMVV207
Plasmid#215343PurposegRNA expression for S. stutzeri nifL deletion on an RSF1010 backboneDepositorInsertS. stutzeri nifL gRNA
UseCRISPR and Synthetic BiologyTagsNoneExpressionBacterialPromoterJ23119Available SinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBBR4pemIK-GW
Plasmid#126929PurposeBroad host range vector with toxin-antitoxin stability system for maintenance in the absence of antibiotic selectionDepositorTypeEmpty backboneExpressionBacterialAvailable SinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
SIRT5.1
Plasmid#13738DepositorAvailable SinceFeb. 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
pPBC-G3-tdT
Plasmid#62814PurposepPBC-G3-tdT expresses PiggyBac inverted terminal repeat-flanked GCaMP3 and tdTomato under the control of the CAG promoter.DepositorInsertITR-CAG-GCaMP3-IRES-tdTomato-ITR
ExpressionBacterial and MammalianPromoterCAGAvailable SinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-FIS
Plasmid#51564PurposeExpression of E. coli FIS for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backboneDepositorInsertFIS
TagsIntein-Chiting Binding DomaninExpressionBacterialAvailable SinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAP003l2
Plasmid#209748PurposeRepA(N99D) SC101 T0-BS7 araC pBAD SD8 NpuC-C[AGGA]LFVY-NpuN-SsrA ProK 5 tRNA operon tetA-orfL blaDepositorInsertNpuC-C[AGGA]LFVY-NpuN-SsrA(rc)
ExpressionBacterialAvailable SinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAA3
Plasmid#128280Purposeasymmetric plasmid partitioning E. coli - target plasmidDepositorInsertPiq - mRFP, parS
ExpressionBacterialAvailable SinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXWJ109Hg-gfp
Plasmid#123148PurposeA mercury sensor with optimized intracellular receptor density. pSB3K3 carrying J109-32merR-t-PmerT-30gfp-tDepositorInsertJ109-32merR-t-PmerT-30gfp-t
UseSynthetic BiologyExpressionBacterialPromoterJ109, PmerTAvailable SinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
MZB174
Plasmid#228487Purpose10xHis-SUMO (Smt3) fused, HRV-3C cleavable, GST-tag for rapid cloning/DNA assembly of pulldown targets via NotI restriction site for E. coli based protein expressionDepositorTypeEmpty backboneTags10xHis-SUMO(Smt3)-GST-HRVExpressionBacterialPromoterT7 PromoterAvailable SinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPPC019.AAV
Plasmid#171147PurposeExpression of J1-BBa_J23117-mRFP and hAAVS1 scRNA on pRK2-KmR plasmidDepositorInsertshAAVS1 scRNA
J1-BBa_J23117-mRFP
UseCRISPRExpressionBacterialPromoterBBa_J23119 and J1-BBa_J23117Available SinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZE31ms2
Plasmid#25860DepositorInsertms2 binding site (x2)
ExpressionBacterialMutationnoneAvailable SinceNov. 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
pPro24
Plasmid#17805DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceApril 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
pETNKI-strepII-3C-LIC-Amp
Plasmid#108707PurposeExpression of a StrepII-tagged target protein with 3C protease cleavage site.DepositorTypeEmpty backboneTagsStrepII-3C protease cleavage siteExpressionBacterialPromoterT7Available SinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
P-BT3332
Plasmid#188110PurposepBolux harboring the promoter region upstream of BT3332DepositorInsertThe B. thetaiotaomicron BT3332 promoter cloned into pBolux
ExpressionBacterialPromoterBT3332Available SinceJan. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDB007(+)
Plasmid#99147PurposeAccessory plasmid for PACE of MjTyrRS variantsDepositorInsertPT7 [SD8] gIII, luxAB; PproK tyrT(Opt, CUA)
ExpressionBacterialPromoterPT7 and PproKAvailable SinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSMART-GFPuv
Plasmid#65822PurposeGFPuv Expression, Low Phosphate Dependent E. coli ExpressionDepositorInsertgfpuv
ExpressionBacterialPromoterE . coli insulated waaH gene promoter, low phosp…Available SinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRW004-SM-destination-proUBQ10-Cas9
Plasmid#104438PurposeDestination vector expressing plant-codon-optimized Cas9 under UBQ10 promoter, with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free plants;DepositorTypeEmpty backboneUseCRISPRExpressionBacterial and PlantPromoterUBQ10Available SinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBS-AMVpl-β1WT-nAchR
Plasmid#97080PurposeTo determine if structural domains are important for nicotinic acetylcholine receptor (nAChr) channel function via dose-response curves from voltage-clamped oocytes to estimate EC50's and Hill CoDepositorInsertBeta1 nicotinic acetylcholine receptor (Chrnb1 Mouse)
ExpressionBacterialMutationWTPromoterT7Available SinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUdO4z
Plasmid#210253Purposelow-copy plasmid for bacterial expressionDepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only