34,239 results

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pANY6

Plasmid

#112198

Purpose

This plasmid is used for efficient cloning of DNA, efficient expression of protein in E. coli, and ELP-based protein purification.

Depositor

Yingfeng An

Publication

Gao et al J Agric Food Chem. 2018 Jun 20;66(24):61

Type

Empty backbone

Tags

ELP-tag

Expression

Bacterial

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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Ubiquitin (1-76; T66V, L67N)

Plasmid

#110755

Purpose

Bacterial expression of human Ubiquitin (1-76; T66V, L67N)

Depositor

David Komander

Publication

Gladkova et al EMBO J. 2017 Dec 15;36(24):3555-3572. do

Insert

UBB (UBB Human)

Expression

Bacterial

Mutation

T66V, L67N

Promoter

T7

Available since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pAB076i6

Plasmid

#79200

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Publication

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (no operator) gIII, luxAB; Ppro1 434cI-SH2ABL1

Expression

Bacterial

Promoter

PlacZ-opt (no operator); Ppro1

Available since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pAW204

Plasmid

#113244

Purpose

E. coli expression vector for IHFa F13A + IHFb

Depositor

Jennifer Doudna

Publication

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

IHFa F13A (ihfA E. coli)
IHFb

Expression

Bacterial

Mutation

F13A

Available since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pNB3033

Plasmid

#113397

Purpose

Ty3 Gag3-His tagged, codon optimized for expression in E. coli; . IPTG inducible

Depositor

Suzanne Sandmeyer

Insert

Ty3 Gag3 codon optimized for E. coli expression

Tags

Gag3-6XHis tag

Expression

Bacterial

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pAJM.719

Plasmid

#108520

Purpose

PTtg-YFP Reporter. Nar sensor for use in Marionette strain.

Depositor

Christopher Voigt

Publication

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

PTtg-YFP

Expression

Bacterial

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pAJM.711

Plasmid

#108512

Purpose

PPhlF-YFP Reporter. DAPG sensor for use in Marionette strain.

Depositor

Christopher Voigt

Publication

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

PPhlF-YFP

Expression

Bacterial

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pBTK563

Plasmid

#110612

Purpose

BTK assembled broad-host-range plasmid encoding NanoLuc luciferase protein driven by CP25 promoter

Depositor

Jeffrey Barrick

Publication

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

Nanoluc

Expression

Bacterial

Available since

Aug. 10, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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Endophilin II Full

Plasmid

#47409

Depositor

Peter McPherson

Insert

Endophilin II

Tags

GST

Expression

Bacterial

Promoter

tac

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pAW209

Plasmid

#113188

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array

Depositor

Jennifer Doudna

Publication

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Expression

Bacterial

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pCRISPR_eGFP_314

Plasmid

#111822

Purpose

Knockout eGFP

Depositor

Steve Shih

Publication

Sinha et al Lab Chip. 2018 Jul 24;18(15):2300-2312.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA targeting eGFP 294-314

Use

CRISPR

Tags

mCherry

Expression

Bacterial and Mammalian

Promoter

U6

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pCDFNKI-strepII-3C-LIC-strep

Plasmid

#108708

Purpose

Expression of a StrepII-tagged target protein with 3C protease cleavage site.

Depositor

Titia Sixma

Publication

Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

Type

Empty backbone

Tags

StrepII-3C protease cleavage site

Expression

Bacterial

Promoter

T7

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pCS2+ Integrin beta 1b-mKate

Plasmid

#105975

Purpose

in vitro transcription, Full length zebrafish Integrin beta 1b tagged C terminally with mKate2 using Gateway cloning.

Depositor

Caren Norden

Publication

Sidhaye et al Elife. 2017 Apr 4;6. pii: e22689. doi: 1

Insert

Integrinbeta1b

Tags

mKate

Expression

Bacterial

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pET21-10XHis-GST-HRV-Her2-dL5-Her2

Plasmid

#85624

Purpose

Bacterial expression vector for Z342-dL5(E52D)-Z342 affibody fusion protein (AFA) with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP, AffiFAP)

Depositor

Marcel Bruchez

Insert

10XHis-GST-HRV-Her2-dL5-Her2 (EGFR Human, Schistosoma japonicum, Synthetic)

Tags

10XHis-GST-HRV and The Her2 Affibody and dL5 FAP …

Expression

Bacterial

Mutation

The dL5** FAP is E50D, L89S, also known as E52D/L…

Promoter

T7

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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B2-3

Plasmid

#111470

Purpose

Lys-tRNA synthetase

Depositor

Cheemeng Tan

Publication

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

Lys-tRNA synthetase

Tags

6xHis

Expression

Bacterial

Available since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCON1-ProB.gfp

Plasmid

#112516

Purpose

ProB driven gfpmut3 expression with synTT

Depositor

Olivia Steele-Mortimer

Publication

Cooper et al Front Cell Infect Microbiol. 2017 Nov 16

Insert

ProB gfpmut3 TT

Expression

Bacterial

Promoter

ProB

Available since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pET-30a(+)-Syk-tSH2

Plasmid

#111269

Purpose

express murine Syk tandem SH2 domains (Ser 8 to Gln 264), the unphosphorylated form, in E.coli strain Rosetta 2 (DE3)

Depositor

Carol Post

Publication

Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5

Insert

murine Syk tandem SH2 domains (Ser 8 to Gln 264), the unphosphorylated form (Syk Mouse)

Expression

Bacterial

Promoter

T7

Available since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCB410 (His-Ub-Ate1-4 1A7B)

Plasmid

#104339

Purpose

Express His Tag Ate1-4 1A7B

Depositor

Alexander Varshavsky

Insert

ATE1 (Ate1 Mouse)

Tags

6xHis-Ub

Expression

Bacterial

Available since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pACYC.Taq.1Amb

Plasmid

#102794

Purpose

Expresses Taq DNA polymerases with a premature stop codon

Depositor

Andrew Ellington

Publication

Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

Insert

Taq DNA polymerase

Expression

Bacterial

Mutation

W168Stop; *(see below)

Available since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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pUWL201PW-creDE

Plasmid

#112241

Purpose

Streptomyces/E.coli shuttle expression vector encoding creD and creE

Depositor

Emily Balskus

Publication

Waldman et al J Org Chem. 2018 May 29. doi: 10.1021/ac

Inserts

creD
creE

Expression

Bacterial

Available since

June 28, 2018

Availability

Academic Institutions and Nonprofits only

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