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pRExT2A-CT-PmNG

Plasmid

#232201

Purpose

Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: mNeongreen-3xTy-T2A-PAC-3xTy

Depositor

Calvin Tiengwe

Article

Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

Insert

mNeongreen

Expression

Bacterial

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pEM8315

Plasmid

#213988

Purpose

PrpsM driven expression of mCherry2-L

Depositor

Marc Erhardt

Article

Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

Insert

mCherry2-L

Expression

Bacterial

Promoter

PrpsM

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pRSFDuet1_CylLS_CylM

Plasmid

#208760

Purpose

Expresses modified cytolysin S (mCylLS) in E. coli

Depositor

Wilfred van der Donk

Article

Luo et al Anal Chem. 2024 Jan 30;96(4):1767-1773.

Inserts

CylLS
CylLM

Tags

Hisx6 Tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pMBEC8

Plasmid

#187599

Purpose

Base editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Apramycin resistance

Depositor

Pablo Ivan Nikel

Article

Volke et al Nat Commun. 2022 May 31;13(1):3026. doi:

Insert

nCas9-BEC-UGI; cas6f, gfp

Expression

Bacterial

Promoter

trc

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD-R-GECO1.2

Plasmid

#113937

Purpose

Bacterial expression of red fluorescent Ca2+ sensor R-GECO1.2

Depositor

Robert Campbell, Thomas Hughes

Article

Molina et al Biophys J. 2019 May 21;116(10):1873-1886

Insert

R-GECO1.2

Tags

6xHis

Expression

Bacterial

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pBR322-Pdp1_NTD-ZFD_R

Plasmid

#198277

Purpose

For loading R half of zinc finger deaminase targeting human TRAC locus (ZFD_TRAC-NC-Right-G1397-C_DddA); also contains PVC regulatory genes

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

Pdp1_NTD-ZFD_R

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_MBP-nLucCP-67/68

Plasmid

#113922

Purpose

Plasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 67 and 68

Depositor

Kai Johnsson

Article

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

MBP-NanoLucCP-67/68

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available Since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pXWJ109As(RS-te-AAV)

Plasmid

#123156

Purpose

For TEV-based leakiness regulation. pSB3K3 carrying J109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-te-AAV-t

Depositor

Baojun Wang

Article

Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

Insert

J109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-te-AAV-t

Use

Synthetic Biology

Expression

Bacterial

Promoter

J109, ParsR, PhrpLE

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pMVA2

Plasmid

#119951

Purpose

Expresses recombinant MVA pathway in Escherichia coli

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2018 Dec 18. doi: 10.102

Inserts

mvaE
mvaS
mvaK1
mvaK2
mvaD
idi

Expression

Bacterial

Mutation

A110G

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pEPSA5Cas9syn2

Plasmid

#206963

Purpose

S. aureus genome editing vector removed these two motifs AGGNNNNNGAT, CCAYNNNNNNTGT, CCAYNNNNNNGTA, GGANNNNNNNNTCG, and GWAGNNNNNNGAT

Depositor

Daijie Chen

Article

Yang et al ACS Synth Biol. 2023 Oct 13. doi: 10.102

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pMVV108

Plasmid

#215338

Purpose

Expresses sfGFP under induction with the Salicylic Acid Marionette Cassette (NahRAM + PSalTTC-sfGFP) with a BBR1 backbone.

Depositor

Brian Pfleger

Article

Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

Insert

sfGFP

Use

Synthetic Biology

Tags

None

Expression

Bacterial

Promoter

PSalTTC

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pMBA334

Plasmid

#214746

Purpose

High copy thyA-infA plasmid containing gfp and no antibiotic resistance gene (ARG)

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23119-RiboJ-RBS(21992)-gfpmut3

Expression

Bacterial

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pYC1876

Plasmid

#158722

Purpose

Helper plasmid pYC1640 for C-terminal tagging with degradation tag ssrA

Depositor

Yi-Cheng Sun

Article

Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

Insert

ssrA

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pKD077

Plasmid

#136473

Purpose

SSB-mTur2 IDL fusion expression plasmid

Depositor

James Keck

Article

Dubiel et al Nucleic Acids Res. 2020 Jun 19;48(11):60

Insert

SSB-mTur2 (ssb E. coli)

Tags

mTurquoise2

Expression

Bacterial

Mutation

mTurquoise2 inserted between Phe148 and Ser149

Promoter

T7 promoter

Available Since

April 10, 2020

Availability

Academic Institutions and Nonprofits only

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pGV3340

Plasmid

#170278

Purpose

Expression of mutant HIV-2 RT p51 reverse transcriptase

Depositor

Gregory Van Duyne

Article

Sherrill-Mix et al Genome Biol. 2021 Jun 3;22(1):169. doi:

Insert

HIV-2 RT p51 (mutant)

Tags

Hexahistidine

Expression

Bacterial

Promoter

T7-lac

Available Since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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pTn7-SCOUT21.RY

Plasmid

#201006

Purpose

pLv1-pUC18R6KT-miniTn7-Gm-mCherry-sYFP2, GmR, ApR

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Inserts

mcherry
sYFP2

Expression

Bacterial

Promoter

J23104

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTn7-SCOUT21.YB

Plasmid

#201008

Purpose

pLv1-pUC18R6KT-miniTn7-Gm-sYFP2-TagBFP, GmR, ApR

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Inserts

sYFP2
TagBFP

Expression

Bacterial

Promoter

J23104

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGEM cAgg (CT#689)

Plasmid

#13945

Depositor

Cliff Tabin

Article

Hartmann et al Cell. 2001 Feb 9. 104(3):341-51.

Insert

Aggrecan (ACAN Chicken)

Expression

Bacterial

Mutation

Nucleotides 113-800

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pET28C-tCorn

Plasmid

#124588

Purpose

Bacterial Expression of tRNA-Corn aptamer

Depositor

Samie Jaffrey

Article

Song et al Nat Chem Biol. 2017 Nov;13(11):1187-1194

Insert

tRNA-Corn

Expression

Bacterial

Promoter

lacI

Available Since

June 22, 2020

Availability

Academic Institutions and Nonprofits only

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pSALECTNK-TEM1(S70A,D179G)/csTEM1

Plasmid

#81163

Purpose

pSALECT plasmid with TEM-1 beta-lactamase fused to TEM-1

Depositor

Timothy Whitehead

Article

Klesmith et al Proc Natl Acad Sci U S A. 2017 Feb 14. p

Insert

TEM-1 beta-lactamase

Tags

Codon swapped TEM-1 from H26 to W290 and His6x

Expression

Bacterial

Mutation

Deleted the sec transport tag. Plasmid encodes H2…

Promoter

lac promoter

Available Since

Feb. 21, 2017

Availability

Academic Institutions and Nonprofits only

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