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33,526 results

Showing: 9181 - 9200 of 33526 results

  8. pQhsCDtRNA-

    Plasmid
    #86015
    Purpose
    Multi-gene expression system with human box C/D proteins with U14
    Depositor
    Hong Li
    Publication
    Peng et al PLoS One. 2014 Jul 31;9(7):e103096. doi:
    Inserts
    U14 (SNORD14A Human)
    15.5K (SNU13 Human)
    fibrillarin (FBL Human)
    NOP56 (NOP56 Human)
    NOP58 (NOP58 Human)
    Use
    Co-expression; purification
    Tags
    6x-His
    Expression
    Bacterial
    Mutation
    amino acids 1-401, amino acids 1-411, and amino a…
    Available since
    Jan. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    86015-plasmid-map-sequence-id-169486

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  14. EPHB1_HUMAN_D0

    Plasmid
    #79694
    Purpose
    This plasmid encodes the kinase domain of EPHB1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.
    Depositor
    John Chodera, Nicholas Levinson, Markus Seeliger
    Publication
    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/
    Insert
    EPHB1 (EPHB1 Human)
    Tags
    His10-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Nov. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles

  15. pBiex PKC - Peptide 8 GFP RFP

    Plasmid
    #83564
    Purpose
    Expresses a GFP-RFP FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.
    Depositor
    Sivaraj Sivaramakrishnan
    Publication
    Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.
    Insert
    PKC alpha (PRKCA Human)
    Tags
    10 nm ER/K Helix, FLAG purification tag, TEV Prot…
    Expression
    Bacterial and Insect
    Mutation
    Only catalytic domain of PKC (aa 335-672)
    Promoter
    T7 Promoter
    Available since
    Nov. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    83564-plasmid-map-sequence-id-166406

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  16. pMT-zic2a-cytoBirA-2A-mCherry_Ras

    Plasmid
    #80064
    Purpose
    Zic2a promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Publication
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Zebrafish biotagging
    Tags
    3x HA
    Expression
    Bacterial
    Promoter
    Zic2a enhancer driving c-fos promoter
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80064-plasmid-map-sequence-id-152615

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  17. pMTB2-NLS-BirA-2A-mCherry_Ras

    Plasmid
    #80067
    Purpose
    Beta-actin2 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Publication
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Zebrafish biotagging
    Tags
    3x HA
    Expression
    Bacterial
    Promoter
    Beta-actin2
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    80067-plasmid-map-sequence-id-152621

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  18. pMTB2-Avi-Cerulean-Rpl10a

    Plasmid
    #79880
    Purpose
    Beta-actin2 promoter driving Avi-tagged protein containing Cerulean protein fused to zebrafish Rpl10a ribosomal unit (Tryon et al. 2012); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Publication
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Cerulean protein fused to zebrafish Rpl10a ribosomal unit (rpl10a Zebrafish)
    Tags
    Avi
    Expression
    Bacterial
    Promoter
    Beta-actin2
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    79880-plasmid-map-sequence-id-149770

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  19. WasCFP /pQE-30

    Plasmid
    #79858
    Purpose
    Cyan-green fluorescent protein with tryptophan-based chromophore in equilibrium between anionic and neutral states.
    Depositor
    Konstantin Lukyanov
    Publication
    Sarkisyan et al Sci Rep. 2012;2:608. doi: 10.1038/srep00
    Insert
    WasCFP
    Tags
    HisTag
    Expression
    Bacterial
    Mutation
    Cyan fluorescent protein mCerulean with substitut…
    Promoter
    T5 promoter / lac operator
    Available since
    Sept. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    79858-plasmid-map-sequence-id-160287

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  20. pAV11K

    Plasmid
    #63184
    Purpose
    pRS416 backbone, URA3 replaced by KanMX, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.
    Depositor
    Jef Boeke
    Publication
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    63184-plasmid-map-sequence-id-104886

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Showing: 9181 - 9200 of 33526 results