30,880 results

1. pTRCHisB-TRF2∆M

   Plasmid

   #50489

   Purpose

   E. Coli N-term HexHis Tagged Protein Expression Vector

   Depositor

   Jack Griffith

   Article

   Bower et al Biochemistry. 2014 Sep 2;53(34):5485-95.

   Insert

   TRF2 Delta Myb (TERF2 Human)
   

   Tags

   6x His, T7 tag, and TEV protease site

   Expression

   Bacterial

   Mutation

   Contains amino acids 3-437

   Promoter

   pTrc

   Available Since

   Feb. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGBW-m4046916

   Plasmid

   #145627

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 nsp6

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 nsp6 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 28, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pJKR-L-mphR

   Plasmid

   #62560

   Purpose

   Macrolide biosensor. Low copy plasmid that expresses GFP proportional to macrolide concentration.

   Depositor

   George Church

   Article

   Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

   Insert

   GFP
   

   Expression

   Bacterial

   Available Since

   Aug. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a(cd226)

   Plasmid

   #177900

   Purpose

   Bacterial expression of his10-tagged human cd226 Intracellular Domain

   Depositor

   Ron Vale

   Article

   Hui et al Science. 2017 Mar 31;355(6332):1428-1433

   Insert

   CD226 (aa 288-336 only) (CD226 Human)
   

   Tags

   10xHis-Thrombin cut site-T7 tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHH0103 BAG3 WW domain

   Plasmid

   #104266

   Purpose

   Bacterial expression of WW domain from BAG3

   Depositor

   Sachdev Sidhu, Marius Sudol

   Insert

   BAG3 WW domain (BAG3 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   codon optimized for expression in bacteria

   Promoter

   tac

   Available Since

   Aug. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEB1-mEYFP

   Plasmid

   #103985

   Purpose

   Plasmid encoding mEYFP

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   mEYFP
   

   Use

   Low copy

   Expression

   Bacterial

   Mutation

   Mutations relative to wild-type GFP (S65G, V68L, …

   Promoter

   proC

   Available Since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. HS-OS-Plk4-mKate2

   Plasmid

   #105944

   Purpose

   heat shock inducible transgenesis, non phoshorylable version of Plk4

   Depositor

   Caren Norden

   Article

   Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

   Insert

   Plk4 (Plk4 Mouse)
   

   Tags

   mKate2

   Expression

   Bacterial

   Mutation

   1002 to 3704 of Plk4/ deletion of S282-S305.

   Available Since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-PLS-KP6_rpoA-U

   Plasmid

   #190967

   Purpose

   Test the U-to-C editing activity of KP6 on AtrpoA editing site, in E. coli

   Depositor

   Bernard Gutmann

   Article

   Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

   Insert

   PLS-KP6
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. HGS

   Plasmid

   #39101

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   HGS (HGS Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pOP3MT

    Plasmid

    #112601

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminal Avi-His8-tag and MBP fusions

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Tags

    Avi - 8His-MBP

    Expression

    Bacterial

    Available Since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. p3.5VPIII.EGFP.IGR2.1

    Plasmid

    #40865

    Depositor

    Harold Gainer

    Article

    Fields et al J Neurosci. 2003 Aug 27;23(21):7801-9.

    Insert

    Mouse vasopressin gene (Avp Mouse)
    

    Expression

    Bacterial

    Promoter

    AVP

    Available Since

    Nov. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET22-His-TEV-DOT1L-MM

    Plasmid

    #89611

    Purpose

    Bacterial expression vector for an N-terminally His-tagged (TEV-cleavable) version of human Dot1L hypermorphic MM mutant residues 1-416

    Depositor

    Leemor Joshua-Tor

    Article

    Ipsaro et al PLoS One. 2017 Feb 23;12(2):e0172177. do

    Insert

    Dot1L VVEL293MM Mutant (DOT1L Human)
    

    Tags

    His-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGBW-m4046740

    Plasmid

    #145588

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 endoRNAse

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 endoRNAse (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pCyPet-FHA2-AuroraB Substrate-YPet-IRES-puro2b

    Plasmid

    #40731

    Depositor

    Daniel Gerlich

    Article

    Wurzenberger et al J Cell Biol. 2012 Jul 23;198(2):173-83.

    Insert

    Aurora B FRET-sensor
    

    Tags

    CyPet and YPet

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    Oct. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMflET-o4

    Plasmid

    #101314

    Purpose

    Contains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and ereB resistance genes, origin of transfer of RP4 plasmid.

    Depositor

    Sébastien Rodrigue

    Article

    Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7

    Inserts

    oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
    ereB resistance gene
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. LIC1B_Caspase-LOVN7C-7 C450A

    Plasmid

    #104628

    Purpose

    bacterial expression of light-activated caspase-3 with N7C-7 linker variation on C450A mutation

    Depositor

    Jim Wells

    Article

    Smart et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

    Insert

    Caspase-3 (CASP3 Human)
    

    Tags

    His and LOV2

    Expression

    Bacterial

    Mutation

    C450A (dark-locked and light-insensitive mutation)

    Promoter

    T7

    Available Since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. MARK3_HUMAN_D0

    Plasmid

    #79740

    Purpose

    This plasmid encodes the kinase domain of MARK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    MARK3 (MARK3 Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pMJ915v2 + sfGFP

    Plasmid

    #88918

    Purpose

    For expression of modified SpyCas9 in e.coli.

    Depositor

    Jennifer Doudna

    Article

    Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Inserts

    Cas9
    T7
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. AlphaD

    Plasmid

    #124526

    Purpose

    pColE1_AlphaD

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. AlphaB

    Plasmid

    #124524

    Purpose

    pColE1_AlphaB

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge