29,903 results

1. pTac-E-luci

   Plasmid

   #65937

   Purpose

   Theophylline Riboswitch E with luciferase gene

   Depositor

   Justin Gallivan

   Article

   Mishler et al Nucleic Acids Res. 2014 Jun;42(10):6753-

   Insert

   luciferase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pET22-HT/plsA

   Plasmid

   #73625

   Purpose

   Produces Escherichia coli adenylate kinase (PlsA), N-terminal His6 tag

   Depositor

   T. J. Kappock, James Williamson

   Article

   Schultheisz et al ACS Chem Biol. 2008 Aug 15;3(8):499-511.

   Insert

   adenylate kinase (adk Escherichia coli str. K-12 substr. MG1655)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBetI-E1

   Plasmid

   #74677

   Purpose

   BetI-E1 gate

   Depositor

   Christopher Voigt

   Article

   Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

   Insert

   PTac-RBSE1-BetI-PBetI-YFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28-sfGFP-DogTag Loop B

   Plasmid

   #171780

   Purpose

   Expresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Asp102 and Asp103

   Depositor

   Mark Howarth

   Article

   Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

   Insert

   sfGFP-DogTag Loop B
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   DogTag and linkers inserted between residues Asp1…

   Promoter

   T7

   Available since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTHSSe_1

   Plasmid

   #109233

   Purpose

   Empty AmpR pSC101 vector

   Depositor

   Christopher Voigt

   Article

   Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pQLinkHD-SYNZIP6

   Plasmid

   #80662

   Purpose

   Encodes the synthetic coiled coil peptide SYNZIP6

   Depositor

   Amy Keating

   Article

   Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

   Insert

   SYNZIP6
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pQLinkHD-SYNZIP5

   Plasmid

   #80661

   Purpose

   Encodes the synthetic coiled coil peptide SYNZIP5

   Depositor

   Amy Keating

   Article

   Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

   Insert

   SYNZIP5
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSP019

   Plasmid

   #168195

   Purpose

   expresses proteolytically inactive EatA passenger domain with H134R mutation within the protease catalytic triad.

   Depositor

   James Fleckenstein

   Insert

   eatA[H134R]
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   H134R mutation

   Promoter

   araBAD

   Available since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJ211_WT_hi

   Plasmid

   #128850

   Purpose

   High copy number plasmid for expression of E. coli flagellin from its natural promoter

   Depositor

   Mark Pallen

   Article

   Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

   Insert

   Flagellin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJK_proK1_eGFP

    Plasmid

    #59853

    Purpose

    Constitutively expresses eGFP with proK1 promoter

    Depositor

    Timothy Whitehead

    Article

    Bienick et al PLoS One. 2014 Oct 6;9(10):e109105. doi:

    Insert

    Enhanced Green Fluorescent Protein
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    S2R,S65G,S72A

    Promoter

    proK1

    Available since

    Oct. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. TOPOII p48 (DM#261)

    Plasmid

    #15026

    Depositor

    Douglas Melton

    Insert

    p48 (Ptf1a Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 18, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pTW194b63

    Plasmid

    #136930

    Purpose

    Directed evolution of SpCas10

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    npuC-gIII(C)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBbB7a-GFP

    Plasmid

    #35358

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMGA-plux-sfGFP

    Plasmid

    #139936

    Purpose

    AHL-inducible sfGFP expression on M. magneticum/E. coli shuttle vector

    Depositor

    Christopher Voigt

    Article

    Furubayashi et al Adv. Funct. Mater. 2021, 31, 2004813

    Insert

    LuxR-Plux-sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGEM-T-Easy-HA-NLS-dCas9-NLS-SID4x-TaV-2a-Citrine-FRT-Kan-FRT

    Plasmid

    #119065

    Purpose

    Donor construct to amplify HA-NLS-dCas9-NLS-SID4x-TaV-2a-Citrine-FRT-Kan-FRT cassette for BAC recombination

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    HA-NLS-dCas9-NLS-SID4x-TaV-2a-Citrine-FRT-Kan-FRT
    

    Use

    Tags

    HA

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pVC-Ds-ubb:MCP-p65-HSF1-2a-Cerulean-Ds

    Plasmid

    #119074

    Purpose

    Zebrafish ubiquitin promoter driving MCP-p65-HSF1 with Cerulean reporter

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    ubb:MCP-p65-HSF1-2a-Cerulean
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Zebrafish ubiquitin promoter

    Available since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pVC-Ds-ubb:Com-KRAB-2a-Cerulean-Ds

    Plasmid

    #119075

    Purpose

    Zebrafish ubiquitin promoter driving Com-KRAB with Cerulean reporter

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    ubb:Com-KRAB-2a-Cerulean
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Zebrafish ubiquitin promoter

    Available since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pVC-Ds-DrU6a:sgRNA-com-Ds

    Plasmid

    #119077

    Purpose

    To clone sgRNA spacer sequence for microinjections. This sgRNA tracrRNA contains 2x com stem loops

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    U6a:2xCom-sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Zebrafish U6a promoter

    Available since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pVC-Ds-DrU6a:sgRNA-MS2-Ds

    Plasmid

    #119076

    Purpose

    To clone sgRNA spacer sequence for microinjections. This sgRNA tracrRNA contains 2x MS2 stem loops

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    U6a:2xMS2-sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Zebrafish U6a promoter

    Available since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAV104

    Plasmid

    #63194

    Purpose

    pRS404 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Sept. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge