30,880 results

1. pET-NtLAtC60αβC20

   Plasmid

   #160873

   Purpose

   Expression of N. tabacum Rubisco large subunit and A. thaliana Rubisco chaperone protiens cpn60α, cpn60β, cpn20 in E. coli

   Depositor

   Maureen Hanson

   Article

   Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

   Insert

   rbcL, cpn60α, cpn60β, cpn20
   

   Expression

   Bacterial

   Promoter

   rbcL: pBAD, chaperones: T7

   Available Since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. 130_pSKB3-LUMP-P.leioghnati

   Plasmid

   #65894

   Purpose

   Bacterial expression of P. leioghnati LUMP protein, a FRET acceptor protein emitting a cyan-colored fluorescence with an unusually long excited state lifetime

   Depositor

   Gerard Marriott

   Article

   Hoepker et al Proc Natl Acad Sci U S A. 2015 Apr 30. p

   Insert

   LUMP
   

   Expression

   Bacterial

   Available Since

   Aug. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCR8/GW/TOPO PCGF1

   Plasmid

   #69770

   Purpose

   Gateway entry vector for PCGF1

   Depositor

   Gerard Cagney

   Insert

   PCGF1 (PCGF1 Human)
   

   Expression

   Bacterial

   Available Since

   Oct. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pSEVA12DKR-J

   Plasmid

   #58306

   Purpose

   GeneGuard genomic cassette: for constructing ΔdapA::RBS J pi, Kis, FRT-kanR-FRT (high-copy support for R6K COR). Will support pSEVAD1K.

   Depositor

   Tom Ellis

   Article

   Wright et al ACS Synth Biol. 2014 May 13.

   Insert

   Genomic cassette
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   March 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJW4.02

   Plasmid

   #131372

   Purpose

   expression of MBP:PGL-1 in C. elegans

   Depositor

   Geraldine Seydoux

   Article

   Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

   Insert

   pgl-1 (pgl-1 Nematode)
   

   Expression

   Bacterial

   Available Since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. CBP TAZ1(340-439)

   Plasmid

   #173760

   Purpose

   bacterial expression of mouse CBP TAZ1 domain

   Depositor

   Peter Wright

   Article

   De Guzman et al Biochemistry. 2005 Jan 18;44(2):490-7. d

   Insert

   CBP TAZ1 (Crebbp Mouse)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAN-PA4-RFP

   Plasmid

   #62250

   Purpose

   reporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1

   Depositor

   Christopher Voigt

   Article

   Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

   Insert

   mRFP
   

   Expression

   Bacterial

   Promoter

   pA4

   Available Since

   April 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJJS003

   Plasmid

   #188326

   Purpose

   PCR template for repair templates to tag a gene with a mCherry and mIAA7 AID degron using homology-directed repair for C. elegans.

   Depositor

   Mike Boxem

   Article

   Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

   Insert

   mIAA7::mCherry::Linker
   

   Expression

   Bacterial

   Available Since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCH6 - pBait-ChiX

   Plasmid

   #174662

   Purpose

   arabinose-induced synthesis of a hybrid RNA containing a single MS2 hairpin followed by E coli ChiX inserted between XmaI and HindIII sites. Insert can be replaced with RNA bait of interest.

   Depositor

   Katherine Berry

   Article

   Pandey et al Nucleic Acids Res. 2020 May 7;48(8):4507

   Insert

   ChiX
   

   Tags

   1xMS2 hairpin

   Expression

   Bacterial

   Promoter

   pBad

   Available Since

   May 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMTTH+mEGFP-LANA

    Plasmid

    #157801

    Purpose

    Expression of mEGFP and LANA peptide fusion protein

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    mEGFP-LANA
    

    Tags

    His6 and MBP

    Expression

    Bacterial

    Promoter

    pTAC

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. mNG-sfTq2

    Plasmid

    #124218

    Purpose

    Cytoplasmic mNeongreen superfolder mTurquoise2 tandem, ~60 % energy transfer. pNM044 - pSAV057-mNG-sfTq2

    Depositor

    Tanneke den Blaauwen

    Article

    Meiresonne et al Mol Microbiol. 2019 Apr;111(4):1025-1038

    Insert

    mNG-sfTq2
    

    Expression

    Bacterial

    Available Since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pETM6-C4-mCherry

    Plasmid

    #66530

    Purpose

    Expresses mCherry under the control of Mutant T7 Promoter C4

    Depositor

    Mattheos Koffas

    Article

    Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

    Insert

    mCherry
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Codon Optimized for E. coli

    Promoter

    Mutant T7 Promoter - C4

    Available Since

    July 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. BAX KO vector

    Plasmid

    #16575

    Depositor

    Bert Vogelstein

    Article

    Zhang et al Science. 2000 Nov 3. 290(5493):989-92.

    Insert

    BAX knockout (BAX Human)
    

    Expression

    Bacterial

    Available Since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

14. p_SNIPR_target_mut_36

    Plasmid

    #139466

    Purpose

    T7 RNAP-driven expression of a non-cognate SNIPR trigger RNA with a mutation at position 36

    Depositor

    Alexander Green

    Article

    Hong et al Cell. 2020 Feb 25. pii: S0092-8674(20)30

    Insert

    Target_mut36
    

    Expression

    Bacterial

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBAD-pHmGL

    Plasmid

    #191257

    Purpose

    Bacterial expression plasmids for pH sensitive fluorescent protein pHmGL

    Depositor

    Robert Campbell

    Insert

    pHmGL
    

    Tags

    His tag

    Expression

    Bacterial

    Available Since

    Nov. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHH0103 NEDD4 WW domain #1

    Plasmid

    #104208

    Purpose

    Bacterial expression of WW domain #1 from NEDD4

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    NEDD4 WW domain #1 (NEDD4 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available Since

    Feb. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. TOPO CFP

    Plasmid

    #68489

    Purpose

    TOPO construct expressing CFP for generation of GMAP-compatible gene

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    Cyan Fluorescent Protein
    

    Use

    Gmap

    Expression

    Bacterial

    Promoter

    Plac

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAV10.KN

    Plasmid

    #63209

    Purpose

    pCACS backbone (Amp), cloNAT, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Available Since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJJH1260

    Plasmid

    #36913

    Depositor

    Jürgen Heinisch

    Article

    Bakota et al Mol Genet Genomics. 2012 Apr;287(4):313-

    Inserts

    ScURA3
    ScURA3
    

    Expression

    Bacterial, Mammalian, and Y…

    Available Since

    July 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTU2S-b (p15A origin)

    Plasmid

    #74089

    Purpose

    Secondary module (BpiI) site in pTU2-a backbone with p15A (low-copy) origin of replication

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Golden Gate destination vector - Level 2, 3 TU's with secondary module and p15A origin
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    None

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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