30,810 results

1. pRCPam

   Plasmid

   #195861

   Purpose

   Encodes chromogenic protein (CP) with nonsense mutation resulting in amber stop codon at amino acid 62. CP inducible with rhamnose in amber suppressor strains.

   Depositor

   Gregory Phillips

   Article

   DeWolf et al J Microbiol Biol Educ. 2023 Oct 25;24(3)

   Insert

   Amber Chromogenic Protein on pRCPam plasmid
   

   Expression

   Bacterial

   Mutation

   Gln62X

   Available Since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX cycT2b (P#1730)

   Plasmid

   #14603

   Depositor

   Matija Peterlin

   Article

   Kurosu et al Gene. 2004 Dec 8. 343(1):173-9.

   Insert

   cycT2 (CCNT2 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   June 30, 2008

   Availability

   Academic Institutions and Nonprofits only

3. pC239

   Plasmid

   #65953

   Purpose

   N2 inverter plasmid for two-strain system

   Depositor

   Matthew Bennett

   Article

   Chen et al Science. 2015 Aug 28;349(6251):986-9. do

   Inserts

   AiiA
   LacI
   

   Tags

   LAA tagged

   Expression

   Bacterial

   Promoter

   pCin and pCin*

   Available Since

   Aug. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBXCA3GH

   Plasmid

   #47071

   Purpose

   FX cloning E.coli expression vector with araBAD promoter and C-terminal Avi-tag, 3C protease cleavage site, GFP and 10x His tags

   Depositor

   Raimund Dutzler, Eric Geertsma

   Article

   Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

   Type

   Empty backbone

   Tags

   3C protease site (PreScission site), Avi-tag, GFP…

   Expression

   Bacterial

   Promoter

   araBAD

   Available Since

   Jan. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQE80l A-CC-A

   Plasmid

   #196478

   Purpose

   Protein expression of SpyTag-ELP-CarHCC-ELP SpyTag, with GRGD site linked with (GGGGS)2 before CarHCC and after first ELP

   Depositor

   Fei Sun

   Article

   Yang et al Sci Adv. 2022 Apr;8(13):eabm5482. doi: 1

   Insert

   A-CC-A
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. TOPOII p48 (DM#261)

   Plasmid

   #15026

   Depositor

   Douglas Melton

   Insert

   p48 (Ptf1a Mouse)
   

   Expression

   Bacterial

   Available Since

   Oct. 18, 2007

   Availability

   Academic Institutions and Nonprofits only

7. HisMS2_VLP_FluA

   Plasmid

   #175955

   Purpose

   Generation of MS2 Virus-Like Particles packaged with the sequence for the Influenza A Matrix Protein [A/Illinois/20/2018(H1N1)]

   Depositor

   Paul Freemont

   Article

   Unruh et al medRxiv 2024.04.17.24305965

   Insert

   M gene
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 19, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pTYB2-eIF1

   Plasmid

   #37217

   Depositor

   Jon Lorsch

   Article

   Acker et al Methods Enzymol. 2007;430:111-45.

   Insert

   eIF1
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 16, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pAN-OR

   Plasmid

   #62307

   Purpose

   synthetic circuit: OR gate

   Depositor

   Christopher Voigt

   Article

   Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

   Inserts

   A2NT sgRNA
   A4NT sgRNA
   A2NT sgRNA
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   PA2, PPhlF, and pBAD

   Available Since

   April 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBEAST_J23101-bphC

    Plasmid

    #128137

    Purpose

    The cell-free adapted backbone, pBEAST, expressing bphC (the enzyme converting biphenyl-2,3-diol to 2-hydroxy-6-oxo-6-phenylhexa-2,4-) under control of the constitutive promoter J23101, and RBS B0032

    Depositor

    Jean-Loup Faulon

    Article

    Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

    Insert

    bphC
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23101

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBAD-pHoran4

    Plasmid

    #61558

    Purpose

    Orange fluorescent protein based pH indicator in bacterial expression vector

    Depositor

    Robert Campbell

    Article

    Shen et al J Cell Biol. 2014 Nov 10;207(3):419-32.

    Insert

    pHoran4
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Dec. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pGEX Stat6-SH2

    Plasmid

    #46517

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Stat6 (STAT6 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 510-654

    Promoter

    Tac

    Available Since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pETM-11-HsPABPC1_J

    Plasmid

    #146642

    Purpose

    Bacterial Expression of HsPABPC1

    Depositor

    Elisa Izaurralde

    Insert

    HsPABPC1 (PABPC1 Human)
    

    Expression

    Bacterial

    Mutation

    two silent mutations compared to the sequence giv…

    Available Since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEM09

    Plasmid

    #135479

    Purpose

    Spizellomyces Hsp70 promoter driving expression of Hygromycin resistance fused to GFP

    Depositor

    Nicolas Buchler

    Article

    Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

    Insert

    SpunHsp70pr
    

    Tags

    GFP and hph

    Expression

    Bacterial and Yeast

    Promoter

    Spun Hsp70pr

    Available Since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET11b-SpyCatcher-M2e

    Plasmid

    #165990

    Purpose

    SpyCatcher-fused influenza M2 ectodomain with removable HisTag

    Depositor

    Vesa Hytönen

    Article

    Lampinen et al J Nanobiotechnology. 2021 Jan 19;19(1):2

    Insert

    SpyCatcher-M2e
    

    Tags

    HisTag and SpyCatcher

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET15b/GgVcl 1-1066 (full length)

    Plasmid

    #46171

    Depositor

    Susan Craig

    Article

    Bakolitsa et al Nature. 2004 Jul 29;430(6999):583-6. Epu

    Insert

    Vcl (VCL Chicken)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pZac2.1 gfaABC1D-3xHA-SAPAP3

    Plasmid

    #190200

    Purpose

    Expresses SAPAP3 protein fused with an HA tag at the N-terminus in astrocytes

    Depositor

    Baljit Khakh

    Article

    Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

    Insert

    3xHA-SAPAP3 (Dlgap3 Mouse)
    

    Use

    AAV

    Tags

    HA

    Expression

    Bacterial

    Promoter

    gfaABC1D

    Available Since

    April 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAC1750

    Plasmid

    #127984

    Purpose

    AMA1 plasmid with Aspergillus optimized Cpf1, ble selection marker, and USER cassette (PacI/Nt.BbvCI)

    Depositor

    Uffe Mortensen

    Article

    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

    Insert

    Cpf1
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    codon optimized for Aspergillus nidulans

    Promoter

    Aspergillus nidulans tef1 promoter

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMS8: pTns(AvCAST)

    Plasmid

    #168141

    Purpose

    Inducible expression of AvCAST TnsA, TnsB, TnsC, TniQ and TnsD proteins.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Inserts

    AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
    AvTnsD
    

    Expression

    Bacterial

    Available Since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEX-4T1-Claspin-FL-wt

    Plasmid

    #31621

    Depositor

    Aziz Sancar

    Article

    Lindsey-Boltz et al J Biol Chem. 2009 Nov 27. 284(48):33107-

    Insert

    Claspin (CLSPN Human)
    

    Tags

    Flag, GST, and His

    Expression

    Bacterial

    Available Since

    Nov. 9, 2011

    Availability

    Academic Institutions and Nonprofits only