33,128 results
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Plasmid#222726PurposepNP555 - IS621 RE-LE TBL-T5, DBL-WT bRNA in cisDepositorInsertIS621 bridge RNA
TagssfGFPExpressionBacterialAvailable SinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNP561
Plasmid#222723PurposeTarget and Recombinase Golden Gate Landing Pad Backbone for LT-RT with T7 RecombinaseDepositorTypeEmpty backboneTags6xHisExpressionBacterialAvailable SinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mTagBFP2-TEV-Strep-His
Plasmid#210824PurposeBacterial overexpression vector for C-terminally Strep and His tagged mTagBFP2 fluorescent proteinDepositorInsertmTagBFP2
TagsTwinStrep, 10xHisExpressionBacterialPromoterT7Available SinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJMP12042
Plasmid#222354PurposeTn7 integrative vector with PabstBR promoter expression cassette and aprR marker on Tn7 transposon; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.DepositorTypeEmpty backboneExpressionBacterialAvailable SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJBL7045
Plasmid#167216Purposeoverexpression of CueR in E. coli for cell-free copper sensingDepositorInsertCueR
ExpressionBacterialAvailable SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO2_Bacteroidetes-bGSDM-TEV
Plasmid#222101PurposeFor expression of a mutant bacterial gasdermin (bGSDM) encoded by a Bacteroidetes species with a TEV protease cleavage site for controlled activation.DepositorInsertBacteroidetes-bGSDM-TEV ORF
Tags6xHis-SUMO2ExpressionBacterialMutationTEV protease site replaces residues E242–G248PromoterT7Available SinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
vGlut1 peptide- GST
Plasmid#218677PurposeExpresses a GST fusion to vGlut peptide for immunoisolation of glutamatergic vesiclesDepositorAvailable SinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-hph-dCas9
Plasmid#218371PurposeExpression of dCas9 for CRISPR-interferenceDepositorInsertdCas9
UseCRISPRExpressionBacterialPromoterPtetAAvailable SinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
p3015b
Plasmid#209317PurposeExpresses a sgRNA template with a protospacer cloning site flanked by BsaI restriction sites for easy insertion of a targeting cassette.DepositorInsertGroup B Streptococcus-compatible single guide RNA
ExpressionBacterialPromoterXyl/tet promoter that is constitutively activeAvailable SinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGLO-Magenta-AZA
Plasmid#220784PurposeExpresses a magenta chromoprotein (visible light and fluorescence) with a his-tag and an inducible GFP (araBAD-promoter)DepositorInsertmagenta chromoprotein
Tags6xHisExpressionBacterialAvailable SinceJuly 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-SpyCatcher003-TEVs-SpyTag003DA
Plasmid#216283PurposeExpresses SpyCatcher003-TEV site-SpyTag003DA in bacterial cytoplasm, to allow TEV protease to remove SpyTag003DA and thereby uncage SpyCatcher003 reactivity.DepositorInsertSpyCatcher003-TEVs-SpyTag003DA
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher-H-Lock
Plasmid#216285PurposeExpresses DoubleCatcher H-lock in bacterial cells. DoubleCatcher H-lock is a variant of DoubleCatcher in which each SpyCatcher003 moiety is separated by an alpha helical linker.DepositorInsertDoubleCatcher H-Lock
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-SpyCatcher002-MBP
Plasmid#216295PurposeExpresses SpyCatcher002 linked to maltose-binding protein in bacterial cellsDepositorInsertSpyCatcher002-MBP
TagsHis6 tag and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-2H12
Plasmid#221186PurposeHis6-tagged cdGreen expressed from T7 promoter (Internal Lab ID: UJ11308)DepositorInsertcdGreen
ExpressionBacterialPromoterT7Available SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
p2H12Matry-blind
Plasmid#221181PurposecdGreen-Matry(blind) expressed from an aTc-inducible promoter (Internal Lab ID: UJ11209)DepositorInsertcdGreen-Matry(blind)
ExpressionBacterialPromoterPtetAvailable SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
p2H12
Plasmid#221142PurposecdGreen expressed from an aTc-inducible promoter (Internal Lab ID: UJ11204)DepositorInsertcdGreen
ExpressionBacterialPromoterPtetAvailable SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDG04163
Plasmid#216373PurposexhNup214-Nb1t (+2Cys) Nanobody productionDepositorInsertxhNup214-Nb1t (+2Cys)
UseAffinity Reagent/ AntibodyTags14xHis-scSUMOExpressionBacterialAvailable SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSC234
Plasmid#216357PurposexhNup93-Nb3t (+2Cys) Nanobody productionDepositorInsertxhNup93-Nb3t (+2Cys)
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialAvailable SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSC168
Plasmid#216350PurposexhNup35-Nb1t (+2Cys) Nanobody productionDepositorInsertxhNup35-Nb1t (+2Cys)
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialAvailable SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGLO-Pink-SHE
Plasmid#220781PurposeExpresses a raspberry chromoprotein (visible light and fluorescence) with a his-tag and an inducible GFP (araBAD-promoter)DepositorInsertraspberry chromoprotein (mRFP1)
Tags6xHisExpressionBacterialAvailable SinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only