30,808 results

1. pET15TV-L-SaNAS

   Plasmid

   #110803

   Purpose

   Expresses SaNAS a nicotianamine synthase from Staphylcoccus aureus

   Depositor

   Audrey Lamb

   Article

   McFarlane et al Biochemistry. 2017 Nov 14;56(45):5967-59

   Insert

   SAV2469 (SAV2469 )
   

   Tags

   Hexahistidine tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28b-PaNAS

   Plasmid

   #110801

   Purpose

   Expresses PaNAS nicotianamine synthase from pseudomonas aeruginosa

   Depositor

   Audrey Lamb

   Article

   McFarlane et al Biochemistry. 2017 Nov 14;56(45):5967-59

   Insert

   Pa4836
   

   Tags

   Hexahistidine tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET15TV-L-SaODH

   Plasmid

   #110804

   Purpose

   Expresses SaODH and opine dehydrogenase from Staphylococcus aureus

   Depositor

   Audrey Lamb

   Article

   McFarlane et al Biochemistry. 2017 Nov 14;56(45):5967-59

   Insert

   SAV2468 (SAV2468 )
   

   Tags

   Hexahistidine tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. TDRD3 (3PMT)

   Plasmid

   #28274

   Depositor

   Cheryl Arrowsmith

   Insert

   Tudor domain-containing protein 3 (TDRD3 Human)
   

   Tags

   His with TEV cleavage

   Expression

   Bacterial

   Mutation

   contains Tudor domain, residues 553-611.

   Available Since

   April 14, 2011

   Availability

   Industry, Academic Institutions, and Nonprofits

5. pGEX-EZH2-T487A mutant

   Plasmid

   #28061

   Depositor

   Mien-Chie Hung

   Article

   Wei et al Nat Cell Biol. 2011 Jan . 13(1):87-94.

   Insert

   EZH2 (EZH2 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   T487A

   Available Since

   June 6, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pET21a - SodA

   Plasmid

   #89612

   Purpose

   Expresses recombinant 6HIS tagged T. cruzi superoxide dismutase A protein in E. coli upon IPTG induction

   Depositor

   Judy Lieberman

   Article

   Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

   Insert

   Superoxide dismutase A
   

   Tags

   6HIS and T7 tag (gene 10 leader)

   Expression

   Bacterial

   Available Since

   May 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. DARS_pNIC-Bio3

   Plasmid

   #153048

   Purpose

   Expression construct for producing Avi tagged Glycyl-tRNA synthetase

   Depositor

   Susanne Gräslund

   Insert

   DARS (DARS1 Human)
   

   Tags

   Avi and His6-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPhlF-P1

   Plasmid

   #74685

   Purpose

   PhlF-P1 gate

   Depositor

   Christopher Voigt

   Article

   Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

   Insert

   PTac-RBSP1-PhlF-PPhlF-YFP
   

   Expression

   Bacterial

   Available Since

   April 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSrpR-S1

   Plasmid

   #74691

   Purpose

   SrpR-S1 gate

   Depositor

   Christopher Voigt

   Article

   Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

   Insert

   PTac-RBSS1-SrpR-PSrpR-YFP
   

   Expression

   Bacterial

   Available Since

   April 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGBW-m4046833

    Plasmid

    #145649

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp9

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp9 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pBTRCk-pVhb-maACR

    Plasmid

    #215651

    Purpose

    pBBR1 Origin, KanR, with maACR gene under Vhb promoter

    Depositor

    Brian Pfleger

    Article

    Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

    Insert

    acyl-CoA reductase
    

    Tags

    Maltose binding protein and His tag

    Expression

    Bacterial

    Promoter

    Vhb

    Available Since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pACYC-pVhb-vfFadBA

    Plasmid

    #215650

    Purpose

    pACYC Origin, CmR, with vfFadBA gene under Vhb promoter

    Depositor

    Brian Pfleger

    Article

    Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

    Insert

    thiolase (FadA), β-hydroxy-acyl-CoA reductase (FadB)
    

    Expression

    Bacterial

    Promoter

    Vhb

    Available Since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJK_ToeRepG2_N19_trigger

    Plasmid

    #132747

    Purpose

    T7 RNAP-driven expression of second-generation toehold repressor index 19 trigger RNA

    Depositor

    Alexander Green, Peng Yin

    Article

    Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

    Insert

    ToeRepG2_N19_trigger
    

    Expression

    Bacterial

    Available Since

    Nov. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDZ585 pKAN 2x-mCherry

    Plasmid

    #72236

    Purpose

    Floxed Kanamycin (G418) selection maker; 3' tagging vector used to create C-terminal fusion proteins with 2x-mCherry

    Depositor

    Daniel Zenklusen

    Article

    Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

    Insert

    2x-mCherry
    

    Expression

    Bacterial

    Available Since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. BB1_12_YlpGPD

    Plasmid

    #117820

    Purpose

    Promoter pGPD of Y. lipolytica for GoldenMOCS

    Depositor

    Michael Sauer

    Article

    Egermeier et al FEMS Microbiol Lett. 2019 Jan 29. pii: 5

    Insert

    YlpGPD
    

    Expression

    Bacterial

    Available Since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAJM.713

    Plasmid

    #108514

    Purpose

    PLuxB-YFP Reporter. OC6 sensor for use in Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    PLuxB-YFP
    

    Expression

    Bacterial

    Available Since

    June 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX-mUbp43-His

    Plasmid

    #12458

    Depositor

    Dong-Er Zhang

    Article

    Malakhov et al J Biol Chem. 2002 Mar 22. 277(12):9976-8

    Insert

    UBP43 (Usp18 Mouse)
    

    Tags

    GST and His

    Expression

    Bacterial

    Available Since

    Jan. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pMTP140 (pBAD322G_TniQ-Cascade(Tn6900))

    Plasmid

    #164262

    Purpose

    Vector expressing the TniQ, Cas8/5, Cas7, and Cas6 proteins from the Tn6900 element with an arabinose promoter.

    Depositor

    Joseph Peters

    Article

    Petassi et al Cell. 2020 Nov 30. pii: S0092-8674(20)31

    Insert

    Codon-optimized tniQ-cas8/5-cas7-cas6 operon from Tn6900 from A. salmonicida S44 pS44-1
    

    Expression

    Bacterial

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMTP170 (pBAD33_CRISPR(Tn6900)-entry)

    Plasmid

    #164263

    Purpose

    Entry vector for cloning user-defined spacers in the Tn6900 CRISPR array.

    Depositor

    Joseph Peters

    Article

    Petassi et al Cell. 2020 Nov 30. pii: S0092-8674(20)31

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    Arabinose

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDEST17-Tpt1

    Plasmid

    #42571

    Depositor

    Stan Fields, Jay Hesselberth

    Article

    Schutz et al RNA. 2010 Mar;16(3):621-31. Epub 2010 Ja

    Insert

    Tpt1
    

    Tags

    6xHistidine

    Expression

    Bacterial

    Promoter

    T7 RNA Polmyerase

    Available Since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only