31,188 results
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Plasmid#172756PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-37; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-37
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET11b-MIF Y99F
Plasmid#149439PurposeExpression of MIF Y99F in E. coli for protein productionDepositorAvailable SinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
ZP17 Krox20
Plasmid#16924DepositorInsertKrox20 (egr2b Zebrafish)
ExpressionBacterialAvailable SinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMTTH+eGFP-bromo5
Plasmid#157804PurposeExpression of synthetic protein composed of eGFP and 5 repeats of BRD4 bromodomain #1DepositorInserteGFP-bromo5
TagsHis6 and MBPExpressionBacterialPromoterpTACAvailable SinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
Human HMG-CoA lyase (HL)
Plasmid#52433Purposebacterial expression of human HMG-CoA lyase (HL)DepositorAvailable SinceApril 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET15b/BOA
Plasmid#82041PurposeFor E.coli expression of 6xHis-Tagged BOA (Burkholderia oklahomensis agglutinin)DepositorInsertBurkholderia oklahomensis agglutinin
Tags6x HisExpressionBacterialAvailable SinceJune 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
UGDH
Plasmid#38900PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJJS003
Plasmid#188326PurposePCR template for repair templates to tag a gene with a mCherry and mIAA7 AID degron using homology-directed repair for C. elegans.DepositorInsertmIAA7::mCherry::Linker
ExpressionBacterialAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCas9-beta-mmr
Plasmid#169241PurposeCas9, beta, and mutL mutant expression plasmid for introducing chromosomal point mutationsDepositorInsertscas9
beta
mutL-E36K
UseCRISPR and Synthetic BiologyExpressionBacterialMutationChanged glutamic acid 36 to lysineAvailable SinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET30 GLTP
Plasmid#170733PurposeThe plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.DepositorAvailable SinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJSC281 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC2 FRET variant
Plasmid#101230PurposeBacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC2 FRET variantDepositorInsertSpCas9 variant C80S/C574S/E60C/D273C/N692A/M694A/Q695A/H698A
Tags10x His, MBP, and TEV siteExpressionBacterialMutationC80S, C574S, E60C, D273C, N692A, M694A, Q695A and…PromoterT7Available SinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEB2-TagRFP
Plasmid#103997PurposePlasmid encoding TagRFPDepositorInsertTagRFP
UseLow copyExpressionBacterialMutationMutations relative to eqFP578 (R32G, K42R, K67R, …PromoterproCAvailable SinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBAD-pHmGL
Plasmid#191257PurposeBacterial expression plasmids for pH sensitive fluorescent protein pHmGLDepositorInsertpHmGL
TagsHis tagExpressionBacterialAvailable SinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRG5271Neo
Plasmid#26387DepositorTypeEmpty backboneExpressionBacterial and WormAvailable SinceOct. 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
pEB1-sfGFP
Plasmid#103983PurposePlasmid encoding sfGFPDepositorInsertsfGFP
UseLow copyExpressionBacterialMutationMutations relative to wild-type GFP (S30R, Y39N, …PromoterproCAvailable SinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-22b(+)-UTPase
Plasmid#12667DepositorAvailable SinceJan. 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET22b 6His SpyCatcher-GRFT
Plasmid#204502PurposeBacterial expression of GRFT with N-terminal SpyCatcher fusion and GS linkerDepositorInsertGRFT
UseSynthetic BiologyTags6xHis and SpyCatcherExpressionBacterialPromoterT7Available SinceSept. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ESRRG_Hormone-recep
Plasmid#109910PurposeProtein expression and purification of ESRRG_Hormone-recepDepositorInsertESRRG_Hormone-recep (ESRRG Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pCJ209
Plasmid#162682PurposeLidless variant of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) replacing Gly251:Thr472 of MHETase with the 7-residue loop (Trp185:Phe191, PETase numbering) of PETase , codon optimized for expression in E. coli K12, with C-terminal His tag.DepositorInsertMHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) with the lid removed
TagsHisExpressionBacterialMutationReplacement of G251-T472 with W185-F191 from PETa…PromoterT7/lacAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only