34,239 results
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Plasmid#196244PurposeExpression of NLS-rich Cas9 (0 Cys residues, mammalian codon optimized)DepositorInsertCas9
UseCRISPRTagsHA, His6 (N terminal on backbone), NLS, Nucleopla…ExpressionBacterialMutationCys at positions 80 and 574 have been mutated to …Available sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEPSA5Cas9syn2
Plasmid#206963PurposeS. aureus genome editing vector removed these two motifs AGGNNNNNGAT, CCAYNNNNNNTGT, CCAYNNNNNNGTA, GGANNNNNNNNTCG, and GWAGNNNNNNGATDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionBacterialAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMW37: pRS426-R2Bm RNA
Plasmid#209291PurposeContains the R2Bm non-LTR retrotransposon 5'UTR and 3'UTR sequencesDepositorInsertR2Bm 5'UTR and 3'UTR
ExpressionBacterial and YeastPromoterGAL1 GAPDHAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMGU
Plasmid#139933PurposeM. magneticum/E. coli shuttle vector (pMGT and pUC)DepositorInsertsfGFP
ExpressionBacterialPromoterPJ23101Available sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBP-P_tetR-PTet*
Plasmid#204077PurposeType 0 promoter partDepositorInserttetR repressor and PTet* tetracycline-inducible promoter
ExpressionBacterialMutationNoneAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJM919_WT His POLI
Plasmid#201444PurposeLow copy vector for expressing human DNA polymerase iota in E. coli with an N-terminal 6x His tagDepositorInsertDNA polymerase iota (POLI Human)
Tags6x HisExpressionBacterialMutationCoding sequence has been optimised for expression…PromoterlacIqAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLRD11
Plasmid#208263PurposeProduction of sclareol in E. coli MEV20DepositorInsertstHMGR
abCAS
ssSS
ExpressionBacterialMutationabCAS(D537A)Available sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET24a MDH_STRAW
Plasmid#204240PurposeBacterial expression of S. avermitilis malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).Available sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET24a MDH_9CREN
Plasmid#204243PurposeBacterial expression of I. islandicus malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).InsertMDH_9CREN
Tags6X HisExpressionBacterialPromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJB158
Plasmid#202601PurposeRP4-based mobilizable plasmid for the IPTG inducible expression of inserts that can be introduced using BsaI-based Golden Gate cloning. Vector has two origins of replication.DepositorTypeEmpty backboneExpressionBacterialAvailable sinceOct. 27, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
RHOAA-c033
Plasmid#205116PurposeRHOA protein expressionDepositorAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 R196A wgMDH
Plasmid#204271PurposeBacterial expression of R196A wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
Tags6X HisExpressionBacterialMutationR196AAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 R124A wgMDH
Plasmid#204257PurposeBacterial expression of R124A wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
Tags6X HisExpressionBacterialMutationR124AAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBad33_ISDge10_reRNA
Plasmid#205340PurposeBacterial expression of ISDge10 TnpB and its reRNADepositorInsertISDge10 TnpB
ExpressionBacterialAvailable sinceOct. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBad33_ISTfu1_reRNA
Plasmid#205355PurposeBacterial expression of ISTfu1 TnpB and its reRNADepositorInsertISTfu1 TnpB
ExpressionBacterialAvailable sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mOct4 Full length(FL)
Plasmid#206394PurposeExpresses Oct4 with a 6xHis tag for protein purificationDepositorAvailable sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTKEI-Dest-loxP-sfGFP
Plasmid#202715PurposeEmpty expression vector containing Cre recombinase reporter.DepositorInsertPw7-loxP-term-loxP-sfGFP
UseCre/LoxExpressionBacterialPromoterconstitutiveAvailable sinceOct. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
PET-B-HypaSpCas9-NLS-6xHis
Plasmid#207386PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertB-HypaSpCas9
UseCRISPRTags6xHisExpressionBacterialMutationN692A, M694A, Q695A, H698A, amino acids 1005-1013…PromoterT7Available sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
PET-eSpCas9-plus-NLS-6xHis
Plasmid#207380PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInserteSpCas9-plus
UseCRISPRTags6xHisExpressionBacterialMutationK848A, R1060A, amino acids 1005-1013 replaced wit…PromoterT7Available sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
PET-eSpCas9-NLS-6xHis
Plasmid#207379PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInserteSpCas9
UseCRISPRTags6xHisExpressionBacterialMutationK848A, K1003A, R1060APromoterT7Available sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only