31,828 results
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Plasmid#60594Purposeencodes the Int2 gene controlled by PBad promoter and a reversed Int5 gene flanked by the attB/P sites of Int2DepositorInsertCas_2+5
ExpressionBacterialAvailable SinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJL1-MBP-4xDQNAT
Plasmid#128390PurposeIn vitro expression of E. coli MBP with C terminal 4xDQNAT glycosylation sitesDepositorInsertMaltose binding protein
Tags4xDQNAT glycosylation tag and 6xHis tagExpressionBacterialMutationA302T- please see depositor commentPromoterT7Available SinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
R26-CAG-rox-FNF-bpA-3pA-rox-td-sfGFP
Plasmid#180153Purposetd-sfGFP dre reporter with Rosa26 recombineering homology armsDepositorInserttd-sfGFP
UseMouse Targeting; Dre/roxExpressionBacterial and MammalianAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR_full-site
Plasmid#186277PurposeReporter plasmid for full-site selection assayDepositorInsertThiomicrospira leader repeat and modified chloramphenicol selection cassette (removed RBS and start codon))
ExpressionBacterialAvailable SinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBA787
Plasmid#186242Purposesoc-C HR Donor Vector (T4 phage editing)DepositorInsertBBR1 backbone + T4 phage homology
ExpressionBacterialAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
B1-2
Plasmid#111464PurposeMet-tRNA synthetaseDepositorInsertMet-tRNA synthetase
Tags6xHisExpressionBacterialAvailable SinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSL0374 (His-MBP-Cas8_Cas7_Cas6)
Plasmid#130639PurposeExpresses V. cholerae CAST Cas8, Cas7, and Cas6 from a T7 promoter, with N-terminal His10-MBP-TEVsite tag on Cas8 for purification.DepositorInsertVchCas8, VchCas7, VchCas6
UseCRISPR; TransposonTagsHis10-MBP-TEVsite on Cas8ExpressionBacterialPromoterT7Available SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAB143
Plasmid#101679PurposeLAA-tagged mCherry under T7 promoter controlDepositorInsertLAA-tagged mCherry under T7 promoter control
UseSynthetic BiologyExpressionBacterialAvailable SinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
CBP TAZ1(340-439)
Plasmid#173760Purposebacterial expression of mouse CBP TAZ1 domainDepositorAvailable SinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX 4T1 RARalpha
Plasmid#35557DepositorAvailable SinceApril 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
MSP2253
Plasmid#70704PurposeBacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #1DepositorInsertsmammalian codon-optimized KKH variant Staphylococcus aureus Cas9
SaCas9 sgRNA (+84)
UseCRISPRTagsNLS-3xFLAGExpressionBacterialMutationE782K, N968K and R1015H in SaCas9PromoterT7Available SinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
H6SUMO-IntC-P53(304-393)
Plasmid#200316Purposebacterial expression of P53 TET-CTD for segmental labelingDepositorInsertp53 (304-393) (TP53 Human)
TagsHis6-SUMO and designed intein CExpressionBacterialPromoterT7Available SinceMay 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
H6GB1tevP53(1-303)IntN
Plasmid#200312PurposeBacterial expression of P53 NTAD-DBD for segmental labelingDepositorInsertp53 (TP53 Human)
TagsHis6GB1tev and designed intein NExpressionBacterialMutationM133L, V203A, N239Y, N268DPromoterT7Available SinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28-a(+)-6xHis-LSSmCherry1(-13)
Plasmid#219415PurposeFluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysateDepositorInsertLSSmCherry1(-13)
TagsMGHHHHHGGExpressionBacterialMutationK137E, K199EPromoterT7Available SinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
mTB3-Myc tag
Plasmid#210205PurposeType 3 phage-display vector that contains kanamycin resistance and displays the Myc-tag at the N-terminus of mature protein III.DepositorInsertMyc-tag
ExpressionBacterialAvailable SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMB2a
Plasmid#190120PurposeLevel 2 MoClo plasmid for A. baumanniiDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
Z-UBQLN2-7
Plasmid#166563PurposescFv of a human scaffold targeting Ubiquilin-2. Antigen coverage aa 1-624 of 624 aa, full-lengthDepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialPromoterLacZAvailable SinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
m2-26 mTRF2
Plasmid#12300DepositorInsertmTRF2 (Terf2 )
ExpressionBacterialAvailable SinceAug. 11, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCRISPReporter
Plasmid#65007PurposeE. coli reporter vector with MCS for inserting bacterial promoter region through 5' end of gene of interest, upstream and in-frame with reporter cloning siteDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionBacterialPromoternoneAvailable SinceMay 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKP325
Plasmid#159596PurposeExpress E.coli TonB proteinDepositorInserttonB
ExpressionBacterialMutationnoneAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only