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pT7-7 asyn S129E

Plasmid

#36050

Depositor

Hilal Lashuel

Article

Paleologou et al J Biol Chem. 2008 Jun 13;283(24):16895-9

Insert

alpha-synuclein S129E (SNCA Human)

Expression

Bacterial

Mutation

S129E

Promoter

T7

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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pGWB602-RBCS-tagRFP

Plasmid

#213868

Purpose

Binary vector for the expression of RBCS fused to the tagRFPn plants (for Agrobacterium-mediated genetic transformation.)

Depositor

Yutaka Kodama

Article

Ichikawa et al Plant Physiol. 2023 Oct 4:kiad528. doi:

Insert

RIBULOSE BISPHOSPHATE CARBOXYLASE SMALL CHAIN 1A (RBCS) (RBCS1A Mustard Weed)

Expression

Bacterial and Plant

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pJ2072.2_c1

Plasmid

#140675

Purpose

CRISPRlator control (“open ring”)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

Insert

CRISPRlator control (“open ring”)

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pCPP5372

Plasmid

#128733

Purpose

Gateway destination low-copy number plasmid for expression of any effector from avrPto promoter with C-terminal HA tag

Depositor

Alan Collmer

Article

Oh et al J Bacteriol. 2007 Nov;189(22):8277-89. E

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pEC2483 (pSpy1C)

Plasmid

#220248

Purpose

replicative E. coli-S. pyogenes shuttle plasmid for heterologous gene expression

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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MAPK1

Plasmid

#73248

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

MAPK1 (MAPK1 Human)

Tags

His-6-TEV

Expression

Bacterial

Promoter

T7

Available Since

April 21, 2016

Availability

Academic Institutions and Nonprofits only

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pEN019-SpCas9-hiNLS-t-M1M4

Plasmid

#234456

Purpose

Expresses hiNLS variant t-M1M4 inside 0C triNLS-SpCas9-NGG

Depositor

Ross Wilson

Article

Noel et al CRISPR J. 2025 Apr;8(2):105-119. doi: 10

Insert

SpCas9 hiNLS t-M1M4

Use

CRISPR

Tags

His6, Nucleoplasmin, SV40, and c-Myc

Expression

Bacterial

Mutation

Cys at positions 80 and 574 have been mutated to …

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pET-IG-mSA

Plasmid

#80706

Purpose

Expresses monomeric streptavidin (mSA) in bacterial cells

Depositor

Matthieu Sainlos

Article

Chamma et al Nat Commun. 2016 Mar 16;7:10773. doi: 10

Insert

Monomeric streptavidin

Tags

10xHis tag and TEV site

Expression

Bacterial

Promoter

T7

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pScaf-5544.4

Plasmid

#111524

Purpose

pScaf phagemid with insert for generating DNA origami scaffold 5544 bases in length (v4)

Depositor

Shawn Douglas

Article

Nafisi et al Synth Biol (Oxf). 2018 Jan;3(1). doi: 10

Insert

pScaf-KH'

Expression

Bacterial

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pET23a-HsRPS27a

Plasmid

#69561

Purpose

Bacterial expression; in vitro transcription/translation (T7)

Depositor

Jorge Eduardo Azevedo

Article

Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

Insert

RPS27A (RPS27A Human)

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2015

Availability

Academic Institutions and Nonprofits only