Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Bacterial Expression Clear filter

Applied Filters

Bacterial Expression Clear filter

31,561 results

Showing: 1001 - 1020 of 31561 results

  1. CRBN_ΔHBD

    Plasmid
    #232779
    Purpose
    Expresses CRBN_ΔHBD sequence in E. coli cells with cleavable MBP-His tag and deleted internal DDB1 binding domain
    Depositor
    Ivan Dikic
    Article
    Bailey et al Cell Chem Biol. 2024 Nov 20:S2451-9456(2
    Insert
    Cereblon (CRBN Human)
    Tags
    MBP-His6-TEV
    Expression
    Bacterial
    Mutation
    CRBN (residues 47 to 193 and 249 to 436) with GNG…
    Promoter
    T7
    Available Since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232779-plasmid-map-sequence-id-462151

    Enlarge

  2. 803 pGEX2T PTEN

    Plasmid
    #10784
    Depositor
    William Sellers
    Insert
    PTEN (PTEN Human)
    Tags
    GST
    Expression
    Bacterial
    Available Since
    Nov. 14, 2005
    Availability
    Academic Institutions and Nonprofits only

  7. pJB158

    Plasmid
    #248751
    Purpose
    PopuA+20xTetO biosensor cicuit in pNBU2 integrative Bacteroides plasmid
    Depositor
    Carolina Tropini
    Insert
    PBfP1E6-RBS1PLP-mScarletI3-rrnBT2-20xTetO-T7Te-rrnBT1-PopuA-TetR-rrnBT2-pTetR56-RBS1PLP-mGL-rrnBT2
    Expression
    Bacterial
    Available Since
    Jan. 9, 2026
    Availability
    Academic Institutions and Nonprofits only
    248751-plasmid-map-sequence-id-496751

    Enlarge

  8. pEM351

    Plasmid
    #248763
    Purpose
    80xTetO Sponge Plasmid with BsaI sites flanking sponge casette for Golden Gate Assembly
    Depositor
    Carolina Tropini
    Insert
    T7Te-rrnBT1-80xTetO
    Expression
    Bacterial
    Available Since
    Jan. 9, 2026
    Availability
    Academic Institutions and Nonprofits only
    248763-plasmid-map-sequence-id-496748

    Enlarge

  10. pEM414

    Plasmid
    #248766
    Purpose
    20xTetO Sponge Plasmid with BsaI sites flanking sponge casette for Golden Gate Assembly
    Depositor
    Carolina Tropini
    Insert
    T7Te-rrnBT1-20xTetO
    Expression
    Bacterial
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    248766-plasmid-map-sequence-id-496747

    Enlarge

  11. EV-A71 isolate 4643-TW98 2A precursor protease with C110A and four aa at N-term to create a VP1-2A junction in active site

    Plasmid
    #228633
    Purpose
    Bacterial expression of the A71 2A protease with ITTL at the N-terminal and a C110A mutation used for crystallization in P 6 1 (PDB 9FGO)
    Depositor
    ASAP Discovery, Frank von Delft
    Insert
    Enterovirus Coxsackievirus A71 2A protease
    Tags
    His-SUMO
    Expression
    Bacterial
    Mutation
    C110A
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    228633-plasmid-map-sequence-id-464103

    Enlarge

  12. PPE18-ALFA

    Plasmid
    #231247
    Purpose
    Expresses a C-terminally tagged PPE18 (ALFA tag)
    Depositor
    Bryan Bryson
    Insert
    PPE18-ALFA (PPE18 Mycobacterium tuberculosis)
    Tags
    ALFA Tag
    Expression
    Bacterial
    Promoter
    hsp60
    Available Since
    March 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    231247-plasmid-map-sequence-id-463614

    Enlarge

  13. pET28-mEGFP(A206K)-TwStrep

    Plasmid
    #208622
    Purpose
    for E. coli expression of C-ter Twin Strep tagged mEGFP(A206K), EGFP cloned from pEGFP-C1, A206K introduced by seamless (Gibson) clone
    Depositor
    Quanfu Ma
    Insert
    mEGFP(A206K)
    Tags
    6xHis and Twin Strep
    Expression
    Bacterial
    Mutation
    (compared with pEGFP-C1, protein level) A206K
    Promoter
    T7
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208622-plasmid-map-sequence-id-415916

    Enlarge

  18. p405ADH1

    Plasmid
    #15966
    Depositor
    Nicolas Buchler, Fred Cross
    Insert
    p405ADH1 (ADH1 Budding Yeast)
    Expression
    Bacterial and Yeast
    Mutation
    ADH1 promoter is between SacI and XbaI restrictio…
    Available Since
    Nov. 2, 2007
    Availability
    Academic Institutions and Nonprofits only
    15966-plasmid-map-sequence-id-8451

    Enlarge

Showing: 1001 - 1020 of 31561 results