34,239 results
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Plasmid#160672PurposeMobile CRISPRi sfGFP 'test', gmc6 sgRNADepositorInsertgmc6 (gfp) sgRNA
ExpressionBacterialAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJMP2820
Plasmid#160669PurposeMobile CRISPRi sfGFP 'test', empty sgRNADepositorInsertempty sgRNA (BsaI)
ExpressionBacterialAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJMP2782
Plasmid#160668PurposeMobile CRISPRi empty sgRNA (BsaI) for cloning new sgRNAsDepositorTypeEmpty backboneExpressionBacterialAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXZ13lacZalpha
Plasmid#160229Purposeplaces LacZalpha unit between Esp3I sites for better cutting of plasmid and ability to distinguish recombinants as white colonies when plated on Xgal platesDepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterlacAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
SRSF11_RRM_pGEX
Plasmid#135121PurposeRecombinant expression of SRSF11 with dual-affinity tagDepositorAvailable sinceOct. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
R701-X71-527: His6-tev-Hs.CALM1(1-80)
Plasmid#159694PurposeE. coli protein expression of His6-tev-Hs.CALM1(1-80)DepositorAvailable sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
R717-X45-566: His6-MBP-tev-Hs.RAF1(136-188)
Plasmid#159698PurposeE. coli protein expression of His6-MBP-tev-Hs.RAF1(136-188)DepositorAvailable sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRibo-Tt
Plasmid#154124PurposeExpresses wt Ribo-T rRNA and a set of tRNAs in bacterial cellsDepositorInsertsengineered bacterial rRNAs operon under control of pL promotor
bacterial tRNAs (Asp, Trp, Ile, Ala, Glu) operon under control of Ptac promoter
UseSynthetic BiologyExpressionBacterialPromoterPtac and pLAvailable sinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKP325
Plasmid#159596PurposeExpress E.coli TonB proteinDepositorInserttonB
ExpressionBacterialMutationnoneAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
R701-X54-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12D
Plasmid#159562PurposeE. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12DDepositorInsertHis6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12D (KRAS Human)
TagsHis6-MBP-tev-AviExpressionBacterialMutationG12DAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
R989-X28-566: His6-MBP-tev-G-Hs.KRAS4b(1-188) G12D
Plasmid#159548PurposeE. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) G12DDepositorInsertHis6-MBP-tev-G-Hs.KRAS4b(1-188) G12D (KRAS Human)
TagsHis6-MBP-tevExpressionBacterialMutationG12DAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
R701-X21-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G12D
Plasmid#159569PurposeE. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G12DDepositorInsertHis6-MBP-tev-Avi-Hs.KRAS4b(2-188) G12D (KRAS Human)
TagsHis6-MBP-tev-AviExpressionBacterialMutationG12DAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
R989-X11-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) G13D
Plasmid#159543PurposeE. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) G13DDepositorInsertHis6-MBP-tev-G-Hs.KRAS4b(1-169) G13D (KRAS Human)
TagsHis6-MBP-tevExpressionBacterialMutationG13DAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZmUbi-5'UTR
Plasmid#154057PurposeLevel 0 Golden Gate vector, containing the ZmUbi promoter and 5' UTR with Level 0.5-compatible overhangs (Wheat construct)DepositorInsertPromoter and 5' UTR Ubiquitin (Zea mays)
UseSynthetic BiologyExpressionBacterialPromoterN/AAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKL12-GCaMP6f-pHuji bb118
Plasmid#158981PurposeConstitutive expression (sigma 70 promoter) of GCaMP6f tethered to pHuji fluorophores in E. coli.DepositorInsertGCaMP6f-pHuji
ExpressionBacterialMutationcodon optimized for e. coli from Addgene#100833 a…PromoterBba_J23118 (sigma 70)Available sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
eNS2B47NS3 L30SF31S
Plasmid#155319PurposeDENV4 47 peptides from NS2B cofactor linked to NS3 full length by VKTQR linker L30SF31S mutantDepositorInsertDENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
TagsHis tagExpressionBacterialMutationL30 to S, F31 to SPromoterM13Available sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
gNS2B47NS3 L30SF31S
Plasmid#155316PurposeDENV4 47 peptides from NS2B cofactor linked to NS3 full length by GGGGSGGGG linker L30SF31S mutantDepositorInsertDENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
TagsHis tagExpressionBacterialMutationL30 to S, F31 to SPromoterM13Available sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDOC-K-glmS-lacIZ
Plasmid#158061PurposeDonor plasmid for insertion of the lacIZ operon and lac promoter into the S. Typhimurium chromosomeDepositorInsertlacIZ operon
UseSynthetic BiologyExpressionBacterialPromoterlacAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJ2042.2
Plasmid#140670Purpose3-color stripeDepositorInsert3-color stripe
UseSynthetic BiologyExpressionBacterialAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pdUA-lacIN
Plasmid#141079PurposePlasmid vector for testing DNA binding proteins by the I-Block assay.DepositorTypeEmpty backboneUseVector for the i-block assay designed to study dn…ExpressionBacterialPromoterEscherichia coli lacIN promoterAvailable sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only