34,239 results

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pHCKan-yibDp2-GFPuv

Plasmid

#139083

Purpose

Low phosphate inducible promoter yibDp2 for GFP expression

Depositor

Michael Lynch

Publication

Moreb et al ACS Synth Biol. 2020 Jun 19;9(6):1483-14

Insert

yibDp2-GFPuv

Expression

Bacterial

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pMC060-HisMS2_PLP_Env_pac

Plasmid

#155040

Purpose

Generation of MS2 Virus Like Particles packaged with the sequence for the SARS-CoV-2 Envelope Gene

Depositor

Paul Freemont

Publication

Crone et al Nat Commun. 2020 Sep 8;11(1):4464. doi:

Inserts

Maturation Protein
Coat Protein Dimer
Envelope Gene

Expression

Bacterial

Mutation

Remove TypeIIs Restriction Sites

Promoter

T7

Available since

Sept. 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGEX-HA-GlpG

Plasmid

#155135

Purpose

Expresses N-terminally GFP- and HA-tagged E. coli GlpG from pGEX 4P-1

Depositor

Sinisa Urban

Publication

Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

Insert

GlpG (glpG Escherichia coli)

Tags

GST and HA

Expression

Bacterial

Promoter

tac

Available since

Sept. 8, 2020

Availability

Academic Institutions and Nonprofits only

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PET.SUMO eIF4G1 1-200

Plasmid

#158791

Purpose

Bacterial expression vector for the expression of N-terminal 6xHIS-SUMO-tagged codon optimized eIF4G1 amino acids 1-200 isoform lacking the microexon.

Depositor

Benjamin Blencowe

Publication

Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

Insert

eIF4G1 (codon optimized)

Tags

6xHIS and SUMO

Expression

Bacterial

Mutation

amino acids 1-200

Promoter

T7

Available since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pPP378

Plasmid

#158797

Purpose

pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-1(D371A) in MCS1 for expression in E. coli and protein purification

Depositor

Jennifer Doudna

Publication

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

dCasPhi-1 (D371A)

Expression

Bacterial

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-UAP56

Plasmid

#157661

Purpose

Expresses UAP56 in E.coli cell

Depositor

Byung Il Lee

Publication

Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

Insert

Spliceosome RNA helicase DDX39B (DDX39B Human)

Tags

GST tag

Expression

Bacterial

Mutation

deleted amino acids 1-43

Available since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pCD564

Plasmid

#153026

Purpose

dxCas9(3.7), MCP-SoxS(R93A/S101A)

Depositor

Jesse Zalatan

Publication

Fontana et al Nat Commun. 2020 Apr 1;11(1):1618. doi:

Insert

dxCas9(3.7), MCP-SoxS(R93A/S101A)

Use

CRISPR

Expression

Bacterial

Mutation

SoxS has R93A and S101A mutations

Available since

Aug. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pET21-tVYN-Nt∆

Plasmid

#154431

Purpose

Plasmid used to express the tVYN-Nt∆ luminescent biosensor for use in live cell cyclic di-GMP detection

Depositor

Ming Hammond

Publication

Dippel et al ACS Chem Biol. 2020 Mar 25. doi: 10.1021

Insert

VenusΔC12-NtYcgRΔ-NLucΔ4

Expression

Bacterial

Promoter

T7

Available since

Aug. 13, 2020

Availability

Academic Institutions and Nonprofits only

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S2-77 pComb3xss anti-cFos nanobody

Plasmid

#145834

Purpose

Bacterial expression of anti-cFos (Human) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Publication

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-cFos (Human) recombinant llama nanobody S2-77 (FOS L. glama (llama))

Tags

6xHis-HA

Expression

Bacterial

Promoter

lac

Available since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pMTTH+eGFP-bromo1

Plasmid

#157805

Purpose

Expression of synthetic protein composed of eGFP and BRD4 bromodomain #1

Depositor

Michael Rosen

Publication

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

eGFP-bromo1

Tags

His6 and MBP

Expression

Bacterial

Promoter

pTAC

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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C2-82 pComb3xss anti-cFos nanobody

Plasmid

#145838

Purpose

Bacterial expression of anti-cFos (Human) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Publication

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-cFos (Human) recombinant llama nanobody C2-82 (FOS L. glama (llama))

Tags

6xHis-HA

Expression

Bacterial

Promoter

lac

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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S1-71 pComb3xss anti-cFos nanobody

Plasmid

#145832

Purpose

Bacterial expression of anti-cFos (Human) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Publication

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-cFos (Human) recombinant llama nanobody S1-71 (FOS L. glama (llama))

Tags

6xHis-HA

Expression

Bacterial

Promoter

lac

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pET41a iGluf

Plasmid

#119833

Purpose

Production of recombinant iGluSnFR E25D fast variant, a single-wavelength glutamate indicator, in E. coli

Depositor

Katalin Torok

Publication

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

iGluf

Tags

GST

Expression

Bacterial

Mutation

E25D

Promoter

T7

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pMTTH+mEGFP-LANA

Plasmid

#157801

Purpose

Expression of mEGFP and LANA peptide fusion protein

Depositor

Michael Rosen

Publication

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

mEGFP-LANA

Tags

His6 and MBP

Expression

Bacterial

Promoter

pTAC

Available since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pETM33_Nsp3a_Ub1

Plasmid

#156467

Purpose

Bacterial expression of Sars-CoV2 Nsp3a_Ub1 protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Publication

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Nsp3a_Ub1 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pETM33_Orf10

Plasmid

#156486

Purpose

Bacterial expression of Sars-CoV2 Orf10 protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Publication

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Orf10 (ORF10 Severe acute respiratory syndrome coronavirus 2, Synthetic)

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pUC19/DdmEGFP

Plasmid

#154304

Purpose

cloned with the codon-optimized mEGFP gene for expression in Dictyostelium discoideum

Depositor

Shigehiko Yumura

Publication

Kondo et al Biotechniques. 2020 Feb;68(2):91-95. doi

Insert

mEGFP

Expression

Bacterial

Available since

Aug. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pUC19/mlcR25-mEGFP

Plasmid

#154295

Purpose

Fluorescent reporter for conony screening without an inducer (e.g. IPTG); TED module (Plac-lacO-mlcR25-mEGFP)

Depositor

Shigehiko Yumura

Publication

Kondo et al Biotechniques. 2020 Feb;68(2):91-95. doi

Insert

25-bp fragment of the 3′ end of mlcR (mlcR25); mEGFP

Expression

Bacterial

Available since

July 31, 2020

Availability

Academic Institutions and Nonprofits only

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C331

Plasmid

#141123

Purpose

RhlI expressing plasmid

Depositor

Matthew Bennett

Publication

Alnahhas et al Nat Commun. 2020 Jul 21;11(1):3659. doi:

Insert

RhlI (rhlI )

Tags

ssrA degradation tag

Expression

Bacterial

Promoter

engineered lac promoter

Available since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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pRBS wt

Plasmid

#154126

Purpose

Expresses wt rRNAs operon in bacterial cells

Depositor

Alexander Mankin

Publication

Aleksashin et al Nat Commun. 2020 Apr 20;11(1):1858. doi:

Insert

wt bacterial rRNAs operon under control of pL promotor

Use

Synthetic Biology

Expression

Bacterial

Available since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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