31,059 results
-
Plasmid#167266PurposeOverexpression of HaloTag7 in E. coliDepositorInsertHaloTag7
TagsHis x10ExpressionBacterialPromoterT7Available SinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAB094a
Plasmid#79241PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPBAD rpoZ-HA4; PC araC
ExpressionBacterialPromoterPBAD; PCAvailable SinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET His6 MBP TEV HDAC8_374
Plasmid#122174PurposeExpresses a truncated construct of histone deacetylase 8 for crystallography bearing an N-terminal tobacco etch virus protease-cleavable hexahistidine-maltose binding protein tagDepositorInsertHis6 MBP TEV HDAC8_374 (HDAC8 Human)
Tagshexahistidine tag and tobacco etch virus protease…ExpressionBacterialMutationResidues Met1–Pro7 and H375-V377 have been delete…PromoterT7Available SinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
prhaBAD-anti-rabbit-Nb-Hia5
Plasmid#239627PurposeRhamnose inducible expression plasmid for anti-rabbit nanobody fusion to Hia5 DNA adenine methyltransferaseDepositorInsertHia5
Tags6HIS, MBP, TEV, and anti-Rabbit NanobodyExpressionBacterialMutationCodon optimized for bacterial expressionPromoterrhaBADAvailable SinceJuly 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_NAP1L1-Halo
Plasmid#175342PurposeProduction of HaloTag fusion proteinDepositorAvailable SinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-Sac-Abeta(M1-42)
Plasmid#71875PurposeExpresses the amyloid-beta protein (42 aa), containing an exogenous methionine (Abeta(M1-42)), when expressed in BL21* DE3 p LysS E. coli cellsDepositorAvailable SinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET23b-15F11-HA-mEGFP-6xHis
Plasmid#129593PurposeThe encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.DepositorInsertAnti-HA frankenbody-mEGFP (15F11-HA scFv-mEGFP)
Tags6xHis-tagExpressionBacterialPromoterT7Available SinceAug. 15, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHyo245
Plasmid#173147PurposeExpresses pheS_A294G by dual T7 promoter in e.coliDepositorInsertpheS_A294G
ExpressionBacterialAvailable SinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDB179
Plasmid#91960Purposeempty expression vector for 10His-SUMO tag protein purificationDepositorTypeEmpty backboneTags10-His and Smt3ExpressionBacterialPromoterT7Available SinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUCP20T-tdTomato
Plasmid#78469PurposeBroad range bacterial fluorescence labelingDepositorInserttdTomato
ExpressionBacterialAvailable SinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBb(RSF1010)1k-GFPuv
Plasmid#106395PurposeIPTG-inducible trc promoter expressing gfpuv on RSF1010 originDepositorInsertGFPuv
ExpressionBacterialPromotertrcAvailable SinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
Fucci(CA) hCdt1-iRFP hGeminin-iRFP
Plasmid#190182PurposeFluorescent reporter vector for visualization of G0 and other cell cycle phases.DepositorInsertsExpressionBacterial and MammalianPromoterCMVAvailable SinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIA33
Plasmid#120803PurposeE. coli-C. difficile shuttle vector for CRISPR interference (CRISPRi) in C. difficile; sgRNA targets rfp; Pxyl::dCas9-opt Pgdh::sgRNA-rfpDepositorInsertsdeactivated nuclease dCas9, codon-optimized for C. difficile
single guide RNA targeting red fluorescent protein
UseCRISPRExpressionBacterialMutationBase-pairing region targets red fluorescent prote…PromoterPgdh and PxylAvailable SinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pWaldo-GFPe-SH1446
Plasmid#112507PurposeExpresses SH1446 in E. coli with a C-terminal TEV-GFP-His(8) tag.DepositorInsertPOT family transporter
TagsC terminal TEV GFP octa-His affinity tagExpressionBacterialPromoterT7Available SinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mCherry-CNA35
Plasmid#61607Purposebacterial expression of N-terminal fusion protein of CNA35 with mCherryDepositorInsertmCherry and CNA35
TagsHis tagExpressionBacterialPromoterT7Available SinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
-
pGNW2
Plasmid#122086Purposesuicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cellsDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceApril 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAH01
Plasmid#128162PurposeIncP-based broad host range vector used for inducible expression of genes in methanotrophic bacteriaDepositorTypeEmpty backboneExpressionBacterialPromoterPtetAvailable SinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCV020-tetQ
Plasmid#232826PurposeRandom transposon mutagenesis vector for Bacteroidales species - tetracyclineDepositorInserttetQ
ExpressionBacterialAvailable SinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEHlyA5
Plasmid#168298PurposeSecretion of nanobodies and other polypeptides fused to C-terminal secretion signal of HlyADepositorInsertN‑terminal His tag, VHH, HA and E‑tags, C‑HlyA
TagsE-tag, HA-tag, His-tag, Prescission site, and VHH…ExpressionBacterialPromoterLacI-PlacAvailable SinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only