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pGEX-parkin WT

Plasmid

#45969

Depositor

Kalle Gehring

Publication

Trempe et al Science. 2013 May 9.

Insert

Parkin (Prkn Rat)

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression

Promoter

tac

Available since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only

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GST-Hsp90 C(626-732)

Plasmid

#22483

Depositor

William Sessa

Publication

Fontana et al Circ Res. 2002 May 3. 90(8):866-73.

Insert

Hsp90alpha C domain (HSP90AA2P Human)

Tags

GST

Expression

Bacterial

Available since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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GFP-PIPK1 gamma 87

Plasmid

#22300

Depositor

Pietro De Camilli

Publication

Di Paolo et al Nature. 2002 Nov 7. 420(6911):85-9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

human phosphatidylinositol phosphate kinase type 1 gamma 87 (PIP5K1C Human)

Tags

GFP

Expression

Bacterial and Mammalian

Available since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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PET30B-PTEN

Plasmid

#20741

Depositor

Alonzo Ross

Publication

Redfern et al Biochemistry. 2008 Feb 19. 47(7):2162-71

Insert

PTEN (PTEN Human)

Tags

6xHis

Expression

Bacterial

Available since

April 2, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pQE60-Exp5

Plasmid

#12553

Depositor

Ian Macara

Publication

Brownawell et al J Cell Biol. 2002 Jan 7. 156(1):53-64.

Insert

EXPORTIN-5 (XPO5 Human)

Tags

HIS

Expression

Bacterial

Mutation

NONE

Available since

Oct. 2, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pAWP78-PVCpnf_pvc13-Ad5RGDPK7

Plasmid

#198291

Purpose

Produces PVCs retargeted with expanded tropism hAd5 knob (RGD and PK7 insertions)

Depositor

Feng Zhang

Publication

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-Ad5RGDPK7

Expression

Bacterial

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pHyo094 (eMutaT7)

Plasmid

#173124

Purpose

Expresses eMutaT7 for mutagenizing genes downstream from a T7 promoter in e.coli

Depositor

Seokhee Kim

Publication

Park et al Nucleic Acids Res. 2021 Apr 6;49(6):e32.

Insert

pmCDA1-T7RNAP

Expression

Bacterial

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pQE60-RRF-His

Plasmid

#124132

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Publication

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Ribosome recycling factor (frr Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMRE-Tn7-145

Plasmid

#118561

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Publication

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mScarlet-I

Use

Minitn7 delivery vector

Expression

Bacterial

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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His6-SpyCatcher-mi3

Plasmid

#113043

Purpose

Expresses His6-SpyCatcher-mi3 in bacterial cells as platform for plug-and-display vaccination

Depositor

Mark Howarth

Publication

Bruun et al ACS Nano. 2018 Jul 20. doi: 10.1021/acsn

Insert

His6-SpyCatcher-mi3

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pWUR790

Plasmid

#101723

Purpose

Synthetic codon optimized P. furiosus ago with N-terminal Strep(II)-tag in pCDF-1b vector, expression vector for PfAgo

Depositor

John van der Oost

Publication

Swarts et al Nucleic Acids Res. 2015 May 26;43(10):51

Insert

PfAgo

Tags

Strep(II)-tag

Expression

Bacterial

Promoter

T7 Promotor

Available since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTECH-chPylRS(IPYE)

Plasmid

#99222

Purpose

Expression plasmid encoding chPylRS(IPYE) and pylT

Depositor

Dieter Söll

Publication

Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

Insert

Plpp chPyl(IPYE)RS; PproK pylT

Expression

Bacterial

Mutation

Contains activating mutations V31I, T56P, H62Y, A…

Promoter

Plpp and PproK

Available since

Oct. 6, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pBZCas13b

Plasmid

#89898

Purpose

Bacterial expression for bzCas13b, driven by the lac promoter, and DR-spacer-DR sequence driven by js23119. New spacer sequences can be cloned in between the DRs by digesting the plasmid with BsaI.

Depositor

Feng Zhang

Publication

Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.

Insert

Cas13b

Expression

Bacterial

Promoter

Lac

Available since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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PA-Cdc42

Plasmid

#75263

Purpose

Light-activated Cdc42

Depositor

Klaus Hahn

Publication

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cell Division Cycle 42 (CDC42 Human)

Tags

6xHIs, LOV2, and mVenus

Expression

Bacterial, Insect, and Mamm…

Mutation

F56W

Promoter

CMV

Available since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pET28-hTDG

Plasmid

#70758

Purpose

bacterial expression of human TDG

Depositor

Primo Schaer

Publication

Schuermann et al Nucleic Acids Res. 2016 Jan 4. pii: gkv1

Insert

TDG (TDG Human)

Tags

6-HIS

Expression

Bacterial

Promoter

T7/lac operon

Available since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pOPINK-Cezanne (OTU, aa 53-446)

Plasmid

#61581

Purpose

Expresses human Cezanne (OTU domain) in E. coli.

Depositor

David Komander

Publication

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

Cezanne (OTUD7B Human)

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-52 and aa 447-843.

Promoter

T7

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pP3-GFP

Plasmid

#53436

Purpose

Controls GFP expression from the P3 promoter cloned out of S. aureus RN4220 genome

Depositor

Cynthia Collins

Publication

Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

Insert

P3 promoter

Use

Synthetic Biology

Tags

none

Expression

Bacterial

Mutation

none

Promoter

P3

Available since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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pQF

Plasmid

#48095

Purpose

Cumate-inducible expression of 3xFLAG-tagged fusion protein

Depositor

Julia Vorholt

Publication

Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

Type

Empty backbone

Tags

3xFLAG

Expression

Bacterial

Promoter

PQ5

Available since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pET His6 ProteinG TEV LIC cloning vector (1P)

Plasmid

#29660

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, Protein G, and TEV cleavage site

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Nampt-PET28a

Plasmid

#25630

Depositor

Cynthia Wolberger

Publication

Wang et al Nat Struct Mol Biol. 2006 Jul . 13(7):66

Insert

Nampt (Nampt Mouse)

Tags

His6

Expression

Bacterial

Available since

Aug. 9, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article