34,359 results

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pMAL-7xHis-TEV-hexa

Plasmid

#222868

Purpose

Expresses His-tagged mutant (C19V/L56V/C110V/C130S/S135G/S219V) TEV protease in E. coli

Depositor

Nikolaus Loening

Publication

Davis et al Protein Sci. 2025 Mar;34(3):e70049. doi:

Insert

TEV protease

Tags

7xHis and MBP

Expression

Bacterial

Mutation

C19V, L56V, C110V, C130S, S135G, S219V. Insert in…

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pBbAW4k-loxP-TT-loxP-mRFP1

Plasmid

#134405

Purpose

Fluorescent reporter for Cre recombinase activity. Cre removes a transcription terminator, leading to RFP production.

Depositor

Mary Dunlop

Publication

Sheets et al ACS Synth Biol. 2020 Feb 21;9(2):227-235

Insert

W4-loxP-TT-loxP-mRFP1

Use

Cre/Lox and Synthetic Biology

Expression

Bacterial

Promoter

W4 (modified T7A1)

Available since

Jan. 17, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pQE-60NA-msGFP2

Plasmid

#135301

Purpose

Encodes a photostable monomeric superfolder GFP

Depositor

Benjamin Glick

Publication

Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

Insert

msGFP2

Expression

Bacterial

Promoter

T5

Available since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pRSET-mEosEM

Plasmid

#132708

Purpose

application in Correlative light-electron microscopy

Depositor

Pingyong Xu

Publication

Fu et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

Insert

mEosEM

Expression

Bacterial

Available since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pRSFDuet-sumo-h-cGAS

Plasmid

#127161

Purpose

expression of human cGAS protein

Depositor

Thomas Tuschl

Publication

Lama et al Nat Commun. 2019 May 21;10(1):2261. doi:

Insert

MB21D1 (CGAS Human)

Tags

His-SUMO tag

Expression

Bacterial

Mutation

None

Available since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET21a-CysRS-His

Plasmid

#124107

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Publication

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Cysteinyl-tRNA ligase (cysS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pALOD4

Plasmid

#111026

Purpose

Expresses domain 4 of anthrolysin O cholesterol sensor, has N-terminal His6-tag and two point mutations, S404C and C472A

Depositor

Arun Radhakrishnan

Publication

Gay et al Biophys J. 2015 Mar 24;108(6):1459-69. d

Insert

Domain 4 of Anthrolysin O

Tags

His6 tag

Expression

Bacterial

Mutation

Mutations: S404C, C472A

Promoter

T7

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pHis-hTG2

Plasmid

#100719

Purpose

Expression of human transglutaminase 2 in E.coli

Depositor

Byung Il Lee

Publication

Han et al Int J Biol Macromol. 2010 Aug 1;47(2):19

Insert

TGM2 (TGM2 Human)

Tags

His

Expression

Bacterial

Mutation

silent mutation Arg580 (AGG--CGT) to enhance expr…

Promoter

T7

Available since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pTECH-chPylRS(IPYE)

Plasmid

#99222

Purpose

Expression plasmid encoding chPylRS(IPYE) and pylT

Depositor

Dieter Söll

Publication

Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

Insert

Plpp chPyl(IPYE)RS; PproK pylT

Expression

Bacterial

Mutation

Contains activating mutations V31I, T56P, H62Y, A…

Promoter

Plpp and PproK

Available since

Oct. 6, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pBiex-1 GFPNanobody_SNAP

Plasmid

#82711

Purpose

Expresses a GFP binding nanobody linked to a SNAP tag in bacteria and Sf9 cells.

Depositor

Sivaraj Sivaramakrishnan

Publication

Sommese et al Protein Sci. 2016 Aug 18. doi: 10.1002/p

Insert

GFP Nanobody

Tags

6xHis Purification tag, FLAG purification tag, an…

Expression

Bacterial and Insect

Promoter

T7 Promoter

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBS-Int

Plasmid

#50000

Purpose

Expresses the integrase on a suicide plasmid

Depositor

Brian Robertson, Peter Sander

Publication

Springer et al Int J Med Microbiol. 2001 Mar;290(8):669

Insert

Integrase from pMV361

Expression

Bacterial

Promoter

own

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pOPINK-Cezanne (OTU, aa 53-446)

Plasmid

#61581

Purpose

Expresses human Cezanne (OTU domain) in E. coli.

Depositor

David Komander

Publication

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

Cezanne (OTUD7B Human)

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-52 and aa 447-843.

Promoter

T7

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pP3-GFP

Plasmid

#53436

Purpose

Controls GFP expression from the P3 promoter cloned out of S. aureus RN4220 genome

Depositor

Cynthia Collins

Publication

Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

Insert

P3 promoter

Use

Synthetic Biology

Tags

none

Expression

Bacterial

Mutation

none

Promoter

P3

Available since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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GST-Hsp90 C(626-732)

Plasmid

#22483

Depositor

William Sessa

Publication

Fontana et al Circ Res. 2002 May 3. 90(8):866-73.

Insert

Hsp90alpha C domain (HSP90AA2P Human)

Tags

GST

Expression

Bacterial

Available since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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GFP-PIPK1 gamma 87

Plasmid

#22300

Depositor

Pietro De Camilli

Publication

Di Paolo et al Nature. 2002 Nov 7. 420(6911):85-9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

human phosphatidylinositol phosphate kinase type 1 gamma 87 (PIP5K1C Human)

Tags

GFP

Expression

Bacterial and Mammalian

Available since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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PET30B-PTEN

Plasmid

#20741

Depositor

Alonzo Ross

Publication

Redfern et al Biochemistry. 2008 Feb 19. 47(7):2162-71

Insert

PTEN (PTEN Human)

Tags

6xHis

Expression

Bacterial

Available since

April 2, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pQE60-Exp5

Plasmid

#12553

Depositor

Ian Macara

Publication

Brownawell et al J Cell Biol. 2002 Jan 7. 156(1):53-64.

Insert

EXPORTIN-5 (XPO5 Human)

Tags

HIS

Expression

Bacterial

Mutation

NONE

Available since

Oct. 2, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pDW363

Plasmid

#8842

Depositor

David Waugh

Publication

Tsao et al Gene. 1996 Feb 22. 169(1):59-64.

Type

Empty backbone

Tags

MBP and biotin acceptor peptide

Expression

Bacterial

Available since

April 19, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAWP78-PVCpnf_pvc13-Ad5RGDPK7

Plasmid

#198291

Purpose

Produces PVCs retargeted with expanded tropism hAd5 knob (RGD and PK7 insertions)

Depositor

Feng Zhang

Publication

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-Ad5RGDPK7

Expression

Bacterial

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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M13KO7-ΔPS-ΔgeneIII

Plasmid

#134351

Purpose

Helper phage for M13 phage production

Depositor

Mark Isalan

Publication

Brodel et al Sci Adv. 2020 Jun 10;6(24):eaba2728. doi

Insert

Helper phage gene

Expression

Bacterial

Available since

June 11, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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