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in-frame kanamycin backbone

Plasmid

#177149

Purpose

Expresses kanamycin when ligated in-frame

Depositor

Markus Covert

Article

Kudo et al Cell Syst. 2022 May 18;13(5):376-387.e8.

Type

Empty backbone

Expression

Bacterial

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pVRb20_992

Plasmid

#49714

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

sfgfp

Use

Synthetic Biology

Expression

Bacterial

Promoter

Pecf20_992

Available Since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pVRa20_992

Plasmid

#49673

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF20_992

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available Since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pMC051-HisMS2_PLP_pac_LC

Plasmid

#128234

Purpose

Lower Copy expression construct for MS2 phage like particles with Esp3I cloning sites for packaged RNA.

Depositor

Paul Freemont

Article

de Martin Garrido et al Mol Microbiol. 2019 Oct 16. doi: 10.1111

Inserts

Maturation Protein
Coat Protein Dimer

Expression

Bacterial

Promoter

T7

Available Since

Nov. 15, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pCold CL7-Cas12a

Plasmid

#124891

Purpose

obtaining full Cas12a RNP

Depositor

Yi Liu

Article

Qiao et al Commun Biol. 2019 May 3;2:161. doi: 10.1

Insert

Cas12a

Expression

Bacterial

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pET303-colE1TR

Plasmid

#155181

Purpose

Expresses colicin E1 residues 1-364

Depositor

Joanna Slusky

Article

Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi:

Insert

colicin E1 residues 1-364

Tags

C-terminal 6x Histidine tag

Expression

Bacterial

Mutation

truncation of colicin E1 including 1-364

Promoter

T7

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-mCherry-CNA35

Plasmid

#61607

Purpose

bacterial expression of N-terminal fusion protein of CNA35 with mCherry

Depositor

Maarten Merkx

Article

Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

Insert

mCherry and CNA35

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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pVRc20_992

Plasmid

#49739

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

AS20_992

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pLux

Available Since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pEcLeuRSH6

Plasmid

#177591

Purpose

Expresses His tagged LeuRS in Escherichia coli cells

Depositor

Kambiz Hamadani

Insert

Leucine tRNA synthetase

Tags

Hexahistidine

Expression

Bacterial

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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ch-Tog

Plasmid

#28290

Depositor

Linda Wordeman

Article

Moore et al J Cell Biol. 2005 May 9. 169(3):391-7.

Insert

ch-TOG (CKAP5 Human)

Expression

Bacterial and Mammalian

Mutation

K19R, E118G

Available Since

April 8, 2011

Availability

Academic Institutions and Nonprofits only

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pSW002-PpsbA-mNeonGreen

Plasmid

#205017

Purpose

Broad host-range bacterial expression vector with constitutive PsbA promoter; mNeonGreen (codon optimized for P. fluorescens)

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

mNeonGreen

Expression

Bacterial

Mutation

mNeonGreen is codon optimized for expression in P…

Promoter

PpsbA

Available Since

Sept. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pBbA6c_PsulA_sfGFP

Plasmid

#224587

Purpose

SOS response reporter

Depositor

Ophelia Venturelli

Article

Cheng et al Mol Syst Biol. 2023 Mar 9;19(3):e11406.

Insert

sfGFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PsulA

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pFRR5

Plasmid

#167506

Purpose

Broad-Host range expression vector, RSF1010 origin, RhaR-RhaS activator, PrhaBAD promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

RhaS-RhaR-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PrhaBAD

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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Ecoli PancACe

Plasmid

#215712

Purpose

Expresses acetyl-CoA biosensor in Ecoli

Depositor

Katharine Diehl

Article

Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

Insert

PancACe

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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mRFP1-1

Plasmid

#63816

Purpose

A member of an RBS library for mRFP1 with different translation rates

Depositor

Howard Salis

Article

Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

Insert

mRFP1

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

J23100

Available Since

May 15, 2015

Availability

Academic Institutions and Nonprofits only

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5ULO (YWHAZ)

Plasmid

#101258

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

YWHAZ (YWHAZ Human)

Tags

6xHis-TEV

Expression

Bacterial

Promoter

T7

Available Since

Oct. 31, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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His-GFP

Plasmid

#247242

Purpose

A bacterial expression plasmid encoding GFP with N-terminal His

Depositor

Lei Lu

Article

Mahajan et al J Biol Chem. 2023 May;299(5):104604. doi

Insert

EGFP

Tags

6xHis

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Jan. 9, 2026

Availability

Academic Institutions and Nonprofits only

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pMS07

Plasmid

#230070

Purpose

Inducible Promoter (rhamnose-induced, BT3763/rhaR system), replicates via RepA; ermF, C-terminal 3x-FLAG, R6K ori, RP4 oriT

Depositor

Sean Crosson

Article

Schnizlein et al Microbiol Resour Announc. 2025 May 8;14(

Type

Empty backbone

Use

Synthetic Biology

Tags

3xFLAG

Expression

Bacterial

Promoter

rhamnose-inducible (BT3763)

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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MB 101A ttrSR(m13)-sfGFP_mCherry

Plasmid

#232474

Purpose

Optimized tetrathionate sensor with fluorescent reporter (GFP), constitutive mCherry

Depositor

Mikhail Shapiro

Article

Buss et al Nat Commun. 2025 Aug 25;16(1):7931. doi:

Inserts

ttrS
ttrR
PttrB185-269
sfGFP
AxeTxe
mCherry

Expression

Bacterial

Promoter

Constitutive (TTGATAGCTAGCTCAGTCCTAGGTATTGTGCTAGC…

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pMS08

Plasmid

#230072

Purpose

Constitutive expression Promoter (RpoD sigma factor, BT_RS06635), integrates via IntN1; N-/C-terminal mScarlet-I fusion, ermF, C-terminal 3x-FLAG, R6K ori, RP4 oriT

Depositor

Sean Crosson

Article

Schnizlein et al Microbiol Resour Announc. 2025 May 8;14(

Type

Empty backbone

Use

Synthetic Biology

Tags

3xFLAG and mScarlet-I

Expression

Bacterial

Promoter

RpoD (bt_1311/BT_RS06635)

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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