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Plasmid#113431PurposeExpresses 10xHis-MBP fused to LbCas12aDepositorHas ServiceCloning Grade DNAInsertLbCas12a
UseTagsMBPExpressionBacterialMutationPromoterT7Available sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET11phiC31poly(A)
Plasmid#18942DepositorInsertphiC31 integrase (int Streptomyces phage phiC31)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 13, 2008AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003-MBP
Plasmid#133450PurposeExpresses SpyTag003-MBP (maltose binding protein) in bacterial cytoplasmDepositorInsertSpyTag003-MBP
UseTagsHis6 and Thrombin cleavage siteExpressionBacterialMutationPeptide tag added to N-terminus of MBP via a GSGE…PromoterT7Available sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lbu_C2c2_WT
Plasmid#83482PurposeBacterial expression of WT L. buccalis C2c2 CRISPR effector.DepositorInsertLbu_C2c2_WT
UseCRISPRTagsHis6-MBPExpressionBacterialMutationPromoterT7Available sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEB1-wtGFP
Plasmid#103978PurposePlasmid encoding wtGFPDepositorInsertwtGFP
UseLow copyTagsExpressionBacterialMutationPromoterproCAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
IRES-creERT2-FNF
Plasmid#188230PurposeEMCV IRES-cre-ERT2-FRT-Neo*-FRT cassetteDepositorInsertIRES-creERT2-FNF
UseCre/Lox and Mouse TargetingTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNatA (pACYCduet-naa10-naa15)
Plasmid#72928PurposeAllows expression of a modified version of the fission yeast NatA complex - chloramphenicol markerDepositorUseTagsExpressionBacterialMutationCodon optomised for E.coli expressionPromoterT7Available sinceFeb. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
R711-X04-566: His6-MBP-tev-Hs.NF1iso2 (1198-1530)
Plasmid#159579PurposeE. coli protein expression of His6-MBP-tev-Hs.NF1 DEF (1198-1530)DepositorInsertHis6-MBP-tev-Hs.NF1 DEF (1198-1530) (NF1 Human)
UseTagsHis6-MBP-tevExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET30-GBFusion1
Plasmid#133438PurposeStreptococcus GB1 domain in vector for bacterial expression.DepositorInsertGB1 domain
UseTagsExpressionBacterialMutationThis fragment encodes only aa 1 to 56.PromoterAvailable sinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBR BsmAIM Kan + p15A BsaIR Amp
Plasmid#206208PurposeContains two plasmids. Constitutive expression of BsmAIM and inducible expression of BsaIRDepositorInsertBsamAIM and BsaIR
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFS_0453_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)_T7term_ARRAY2_FaqI
Plasmid#117006PurposeExpresses E. coli codon-optimized FsRT-Cas1-Cas2 under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readoutDepositorInsertFusicatenibacter saccharivorans RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBFC1171
Plasmid#231994PurposeCRISPRi-ART crRNA only plasmid encoding crystal violet-inducible crRNA (for dRfxCas13d) with 2xBsaI spacer cloning siteDepositorInsertcrRNA of dRfxCas13d
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterpJEx (Jungle Express)Available sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
L4440_BioBrick-sgRNA
Plasmid#177783PurposeOverexpression of sgRNAs in E. coli HT115, sgRNA overexpression scaffold; for ingestion by C. elegansDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7II-2N4Rtau
Plasmid#177653PurposeRecombinant protein synthesis of human TauDepositorInsertTau (2N4R) (MAPT Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSL4164 (pEffector_IscB,ωRNA)
Plasmid#210874PurposeExpresses GstIscB and native ωRNA from constitutive promotersDepositorInsertGstIscB
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD-smURFP-RBS-HO-1
Plasmid#80341PurposeExpresses smURFP and Heme Oxygenase-1 in bacterial cells.DepositorInsertssmURFP
HO-1
UseTags6 Histidine TagExpressionBacterialMutationPromoterpBADAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEM4Z-EGFP
Plasmid#183475PurposeThis plasmid can be used to prepare EGFP mRNA by In Vitro TranscriptionDepositorInsertEGFP
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM6-RgTALsyn
Plasmid#100947PurposeExpression of Tyrosine Ammonia Lyase from inducible T7 promoterDepositorInsertRgTALsyn
UseSynthetic BiologyTagsExpressionBacterialMutationSilent mutation to remove internal NdeI site on T…PromoterT7Available sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
HHM-iATPSnFR1.0
Plasmid#102546Purposeexpresses ATP sensor in bacteriaDepositorInsertiATPSnFR1.0
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pExp-Trx
Plasmid#112577PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and Trx (thioredoxin (trxA) from E. coli fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneUseTags8xHis-TrxA and Strep tagExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only