30,258 results

1. pSG15-pBAD-tetO-deGFP

   Plasmid

   #102450

   Purpose

   Expresses deGFP fluorescent protein in E. coli. The expression is controlled by a pBAD-tetO combinatorial promoter.

   Depositor

   Richard Murray

   Article

   Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

   Insert

   deGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD-tetO

   Available since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTlpA_mCherry_YafQ/DinJ

   Plasmid

   #229148

   Purpose

   Toxin-Antitoxin (Dinj/YafQ)

   Depositor

   Shrikrishnan Sankaran

   Article

   Dey et al Microb Biotechnol. 2023 Jun;16(6):1264-1

   Insert

   mCherry, DinJ/YafQ
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PtlpA (mCherry), DinJ/YafQ (Native)

   Available since

   Feb. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pK19pA-MN

   Plasmid

   #86973

   Purpose

   ChIC; genomic mapping of chromatin proteins

   Depositor

   Ulrich Laemmli

   Article

   Schmid et al Mol Cell. 2004 Oct 8;16(1):147-57.

   Insert

   protein_A fused to Staphylococcus aureus microccocal nuclease (MNase)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. NylCp2-TS_pET28a

   Plasmid

   #215421

   Purpose

   Expression construct for NylCp2-TS gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   NylCp2-TS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D122G/H130Y/D36A/E263Q

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMRE-Tn7-145

   Plasmid

   #118561

   Purpose

   miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   mScarlet-I
   

   Use

   Minitn7 delivery vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-28b-RfxCas13d-His

   Plasmid

   #141322

   Purpose

   Plasmid for bacterial expression and purification of RfxCas13d protein

   Depositor

   Miguel Angel Moreno-Mateos, Ariel Bazzini

   Article

   Kushawah et al Dev Cell. 2020 Sep 28;54(6):805-817.e7.

   Insert

   Rfx-Cas13d
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFPC1-FLAG-Synaptojanin 1-145

   Plasmid

   #22293

   Depositor

   Pietro De Camilli

   Article

   Perera et al Proc Natl Acad Sci U S A. 2006 Dec 19. 1

   Insert

   Synaptojanin 1_145 (SYNJ1 Human)
   

   Use

   Tags

   Flag and GFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Oct. 21, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. 3xFLAG-CUL3-pCMV7.1

   Plasmid

   #155021

   Purpose

   N-terminal 3xFLAG-tagged CUL3 in a pCMV7.1 vector

   Depositor

   Andre Catic

   Article

   Sweeney et al Sci Rep. 2020 Aug 18;10(1):13942. doi: 1

   Insert

   Cullin 3 (CUL3 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Bacterial

   Mutation

   Promoter

   pCMV7.1

   Available since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pB025

   Plasmid

   #183092

   Purpose

   Expresses FnCas12a in Bacteroides and used for genome editing

   Depositor

   Lei Dai

   Article

   Zheng et al ACS Synth Biol. 2022 Jan 21;11(1):464-47

   Insert

   FnCas12a, gRNA, Promoter, HAB, TetR,
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P1TDPGH023

   Available since

   Aug. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGJK_His-SUMO-LbuCas13a

    Plasmid

    #172488

    Purpose

    Bacterial expression of codon-optimized His-SUMO-LbuCas13a.

    Depositor

    Jennifer Doudna

    Article

    Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

    Insert

    bdSUMO-LbuCas13a (codon-optimized)
    

    Use

    Tags

    His6 and bdSUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter and lac operator

    Available since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX-4T MDM2 WT

    Plasmid

    #16237

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

    Insert

    MDM2 (MDM2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28-MHL

    Plasmid

    #26096

    Purpose

    SGC Empty backbone for bacterial expression

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Use

    Tags

    6xHis and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    Aug. 20, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. APEX2-OMM

    Plasmid

    #79056

    Purpose

    Soybean APEX2 anchored to cytosolic face of outer mitochondrial membrane with C-terminal targeting sequence of last 31aa of human mitochondrial antiviral-signaling protein

    Depositor

    Alice Ting

    Article

    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

    Insert

    Soybean APEX2
    

    Use

    Lentiviral

    Tags

    flag and last 31 amino acids of human MAVS

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV immearly promotor

    Available since

    Aug. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. BirA in pET28a (w400-2)

    Plasmid

    #26624

    Depositor

    Eric Campeau

    Insert

    BirA
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTn Donor

    Plasmid

    #236211

    Purpose

    Arabinose-inducible Tn5 InducTn-seq plasmid

    Depositor

    Matthew Waldor

    Article

    Basta et al Nat Microbiol. 2025 Mar 27. doi: 10.1038

    Insert

    None
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GST-Cdk5

    Plasmid

    #24894

    Depositor

    Harish Pant

    Article

    Amin et al J Neurosci Res. 2002 Feb 1. 67(3):354-62

    Insert

    CDK5 (CDK5 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 17, 2010

    Availability

    Academic Institutions and Nonprofits only

17. Pet28a_Human_H3.3

    Plasmid

    #42632

    Depositor

    Joe Landry

    Insert

    Human H3.3 (H3-3A Human)
    

    Use

    Tags

    Untagged

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 25, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pZE27GFP

    Plasmid

    #75452

    Purpose

    Plasmid for constitutive expression of gfp

    Depositor

    James Collins

    Article

    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

    Insert

    gfp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    placIq promoter

    Available since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. UAS:hGCG-ICAM(1235)- Nrxn1b;cryaa:mCherry

    Plasmid

    #218860

    Purpose

    10xUAS promoter driving expression of human glucagon-truncated ICAM-zfNrxn1b fusion protein for use in trans-Tango.

    Depositor

    Marnie Halpern

    Article

    Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

    Insert

    hGCG-ICAM(1235)-nrxn1b
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSUC01

    Plasmid

    #177204

    Purpose

    chromogenic reporter for gene knockout in Streptomyces

    Depositor

    Wenjun Guan

    Article

    Dong et al J Microbiol Biotechnol. 2021 Dec 28;31(1

    Insert

    SaindC
    

    Use

    Synthetic Biology

    Tags

    NONE

    Expression

    Bacterial

    Mutation

    NONE

    Promoter

    TGTTCACATTCGAACGGTCTCTGCTTTGACAACATGCTGTGCGGTGTTG…

    Available since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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