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pAIP4_2A4D

Plasmid

#234196

Purpose

Heterologous protein expression of ubiquitin-conjugating enzyme E2 variant 1 (UEV-1) (CROC-1) in Escherichia coli

Depositor

Christopher Bahl

Insert

2A4D (UBE2V1 Human)

Tags

10x HisTag-Smt3

Expression

Bacterial

Promoter

T7

Available Since

March 6, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pET-His-VCre

Plasmid

#123131

Purpose

Bacterial expression plasmid for wild-type VCre with an N-terminal His-tag.

Depositor

David Liu

Article

Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

Insert

VCre recombinase

Use

Cre/Lox

Tags

6x His tag

Expression

Bacterial

Promoter

T7

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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pOPINB-hRNF216

Plasmid

#167354

Purpose

Expression of human RNF216 (Triad3A) codon optimized for E. coli and insect cells

Depositor

Bernhard Lechtenberg

Article

Cotton et al Mol Cell. 2022 Feb 3;82(3):598-615.e8. d

Insert

E3 ubiquitin-protein ligase RNF216 (RNF216 Human)

Tags

3C protease cleavage site and 6x-His tag

Expression

Bacterial

Mutation

codon optimised for expression in E. coli

Promoter

T7

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX6p-1-BCCP-PupE

Plasmid

#113403

Purpose

Expresses BCCP-PupE in bacteria

Depositor

Min Zhuang

Article

Liu et al Nat Methods. 2018 Aug 13. pii: 10.1038/s

Insert

BCCP-PupE

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pET22b-TEVP-cleavableELP

Plasmid

#219402

Purpose

Tobacco etch virus protease-responsive elastin-like polypeptide (ELP)

Depositor

Danielle Mai

Article

Wirtz et al Biomacromolecules. 2024 Jul 9. doi: 10.1

Insert

TEVP-cleavableELP

Tags

6xHis

Expression

Bacterial

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pC0058 PinCas13 (B02) His6-TwinStrep-SUMO-BsaI

Plasmid

#115208

Purpose

Expresses PinCas13b from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

PinCas13

Tags

SUMO

Expression

Bacterial

Available Since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pET28b-SpG-His

Plasmid

#179318

Purpose

Plasmid for bacterial expression and purification of SpG-Cas9

Depositor

Julián Cerón, Miguel Angel Moreno-Mateos

Article

Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:

Insert

Human codon-optimized Cas9 variant SpG

Use

CRISPR

Tags

6xHis and SV40-NLS

Expression

Bacterial

Mutation

SpG=D1135L/S1136W/G1218K/E1219Q/ R1335Q/T1337R

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX6P-1-Dendra2

Plasmid

#82436

Purpose

Dendra2 expression plasmid

Depositor

Periklis Pantazis

Article

Mohr et al Nat Protoc. 2016 Dec;11(12):2419-2431. d

Insert

Dendra2

Tags

GST tag

Expression

Bacterial

Promoter

tac

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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p11d-aRPA (MSW#712)

Plasmid

#208078

Purpose

Expression of Alternative human Replication Protein A (RPA1,4,3) in E. coli

Depositor

Marc Wold

Article

Mason et al J Biol Chem. 2009 Feb 20;284(8):5324-31.

Insert

RPA1, RPA4, RPA3 (RPA1, RPA3, RPA4 Human)

Expression

Bacterial

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pOPINS3C

Plasmid

#41115

Depositor

Ray Owens

Article

Bird et al Methods. 2011 Sep;55(1):29-37. Epub 2011

Type

Empty backbone

Expression

Bacterial, Insect, and Mamm…

Available Since

March 27, 2013

Availability

Academic Institutions and Nonprofits only

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