30,804 results

1. pGEX Crk-SH2

   Plasmid

   #46418

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Crk (CRK Human)
   

   Tags

   AviTag, GST, and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 1-124

   Promoter

   Tac

   Available Since

   July 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX Syk(NC)-SH2

   Plasmid

   #46521

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Syk(NC) (SYK Human)
   

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 7-263

   Promoter

   Tac

   Available Since

   July 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBR322 14-3-3 Promoter

   Plasmid

   #16517

   Depositor

   Bert Vogelstein

   Article

   Hermeking et al Mol Cell. 1997 Dec . 1(1):3-11.

   Insert

   14-3-3 promoter (SFN Human)
   

   Expression

   Bacterial

   Available Since

   July 27, 2008

   Availability

   Academic Institutions and Nonprofits only

4. p2CT-His-MBP-Ppr_Cas13a_WT

   Plasmid

   #91872

   Purpose

   Bacterial expression for Cas13a

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

   Insert

   PprCas13a (PprC2c2) WT
   

   Tags

   His6-MBP-TEV site

   Expression

   Bacterial

   Available Since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pACYC-Cas12g1-noncoding

   Plasmid

   #120880

   Purpose

   Expresses concatenated noncoding sequences surrounding the CRISPR-Cas12g1 system in the native genomic loci; expresses the tracrRNA required for activity.

   Depositor

   Arbor Biotechnologies

   Article

   Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

   Insert

   Noncoding sequences for CRISPR-Cas12g1
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   lac

   Available Since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. KaiB

   Plasmid

   #42496

   Depositor

   Michael Rust

   Article

   Phong et al Proc Natl Acad Sci U S A. 2013 Jan 15;11

   Insert

   Kai B
   

   Expression

   Bacterial

   Promoter

   t7

   Available Since

   Feb. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRSF-DUET Cas1 Cas2

   Plasmid

   #80088

   Purpose

   Encodes Cas1 and Cas2

   Depositor

   George Church

   Article

   Shipman et al Science. 2016 Jun 9. pii: aaf1175.

   Inserts

   Cas1 (cas1 E. coli (K12))
   Cas2
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a SV40 ST full length

   Plasmid

   #132636

   Purpose

   expresses SV40 ST full length in E. coli

   Depositor

   Wenqing Xu

   Article

   Cho et al PLoS Biol. 2007 Aug;5(8):e202. doi: 10.1

   Insert

   SV40 small t antigen
   

   Expression

   Bacterial

   Available Since

   Nov. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBPHLWL-GAL4DBD

   Plasmid

   #26256

   Depositor

   Tom Clandinin

   Article

   Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

   Insert

   ZIP- Gal4DBD (GAL4 Budding Yeast)
   

   Use

   Drosophila transgenesis

   Expression

   Bacterial and Insect

   Available Since

   Feb. 2, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pETCON-TNF

    Plasmid

    #67748

    Purpose

    Tumor Necrosis Factor Alpha Yeast Display Plasmid

    Depositor

    Timothy Whitehead

    Article

    Kowalsky et al J Biol Chem. 2015 Oct 30;290(44):26457-7

    Insert

    TNF-Alpha
    

    Expression

    Bacterial and Yeast

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. B6-2

    Plasmid

    #111486

    Purpose

    Pro-tRNA synthetase

    Depositor

    Cheemeng Tan

    Article

    Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

    Insert

    Pro-tRNA synthetase
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    June 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. HOXC9 (murine) HIS-tag pET

    Plasmid

    #8559

    Depositor

    Corey Largman

    Insert

    HOXC9 (Hoxc9 Mouse)
    

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    makes protein by Western

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pQTEV-AIP

    Plasmid

    #31575

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    aryl hydrocarbon receptor interacting protein (AIP Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    Nov. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCA528 SPASTIN , residues 112-196

    Plasmid

    #180627

    Purpose

    Bacterial expression for SPASTIN MIT, residues 112-196. Has N-term HIS-SUMO tag. Used for X-ray crystallography. Internal ID:WISP21-111.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    SPASTIN
    

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 112-196

    Promoter

    T7

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBLO5.01

    Plasmid

    #121418

    Purpose

    pUC19_BsaI-dCas9-BsaI

    Depositor

    David Savage

    Article

    Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

    Insert

    dcas9
    

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pETM41-EcPPK

    Plasmid

    #38334

    Depositor

    Florian Freimoser

    Insert

    E. coli polyphosphate kinase (ppk Escherichia coli)
    

    Tags

    6xHIS, MBP, and TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pWT046a

    Plasmid

    #107897

    Purpose

    W2.2, W2.4-1 plasmid. Contains PTetO-SD8-BE2, PBAD-sgRNA3 and is part of CAMERA 2.2 and 2.4

    Depositor

    David Liu

    Article

    Tang et al Science. 2018 Feb 15. pii: science.aap89

    Insert

    PTetO-SD8-BE2, PBAD-sgRNA3
    

    Expression

    Bacterial

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET23-CBD-(-PP)-EGFP-H246,249D

    Plasmid

    #12598

    Depositor

    Klaus Hahn

    Article

    Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

    Type

    Empty backbone

    Tags

    6xHis and EGFP

    Expression

    Bacterial

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pGEX Hck-SH2

    Plasmid

    #46445

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Hck (HCK Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 134-254

    Promoter

    Tac

    Available Since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pShCAST(Cas12k-TniQ)-sgRNA_entry (CJT11)

    Plasmid

    #181787

    Purpose

    Expresses 3-component ShCAST containing a Cas12k-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    ShTnsB, ShTnsC, ShCas12k-ShTniQ
    

    Expression

    Bacterial

    Mutation

    nAniI = K227M, F80K, L232K

    Promoter

    Lac and J23119

    Available Since

    Feb. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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