30,804 results

1. MAT2AA-c001

   Plasmid

   #53648

   Purpose

   bacterial expression plasmid

   Depositor

   Nicola Burgess-Brown

   Insert

   MAT2AA
   

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   Bacterial expression for structure determination;…

   Available Since

   June 3, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pGEX6-2 SNAP-Syk tSH2

   Plasmid

   #113028

   Purpose

   for purification of Syk tSh2. Purified protein will have a snap tag that can be conjugated to fluorescent dyes.

   Depositor

   Ron Vale

   Article

   Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

   Insert

   SNAP-Syk tSH2
   

   Tags

   SNAP

   Expression

   Bacterial

   Available Since

   Aug. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRG5273Neo

   Plasmid

   #26388

   Depositor

   Denis Dupuy

   Article

   Giordano-Santini et al Nat Methods. 2010 Sep;7(9):721-3. doi: 1

   Type

   Empty backbone

   Expression

   Bacterial and Worm

   Available Since

   Oct. 15, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMGA-plux-sfGFP

   Plasmid

   #139936

   Purpose

   AHL-inducible sfGFP expression on M. magneticum/E. coli shuttle vector

   Depositor

   Christopher Voigt

   Article

   Furubayashi et al Adv. Funct. Mater. 2021, 31, 2004813

   Insert

   LuxR-Plux-sfGFP
   

   Expression

   Bacterial

   Available Since

   July 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEM-T-Easy-HA-NLS-dCas9-NLS-SID4x-TaV-2a-Citrine-FRT-Kan-FRT

   Plasmid

   #119065

   Purpose

   Donor construct to amplify HA-NLS-dCas9-NLS-SID4x-TaV-2a-Citrine-FRT-Kan-FRT cassette for BAC recombination

   Depositor

   Tatjana Sauka-Spengler

   Article

   Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

   Insert

   HA-NLS-dCas9-NLS-SID4x-TaV-2a-Citrine-FRT-Kan-FRT
   

   Tags

   HA

   Expression

   Bacterial

   Available Since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pVC-Ds-ubb:MCP-p65-HSF1-2a-Cerulean-Ds

   Plasmid

   #119074

   Purpose

   Zebrafish ubiquitin promoter driving MCP-p65-HSF1 with Cerulean reporter

   Depositor

   Tatjana Sauka-Spengler

   Article

   Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

   Insert

   ubb:MCP-p65-HSF1-2a-Cerulean
   

   Expression

   Bacterial

   Promoter

   Zebrafish ubiquitin promoter

   Available Since

   Feb. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pVC-Ds-ubb:Com-KRAB-2a-Cerulean-Ds

   Plasmid

   #119075

   Purpose

   Zebrafish ubiquitin promoter driving Com-KRAB with Cerulean reporter

   Depositor

   Tatjana Sauka-Spengler

   Article

   Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

   Insert

   ubb:Com-KRAB-2a-Cerulean
   

   Expression

   Bacterial

   Promoter

   Zebrafish ubiquitin promoter

   Available Since

   Feb. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pVC-Ds-DrU6a:sgRNA-com-Ds

   Plasmid

   #119077

   Purpose

   To clone sgRNA spacer sequence for microinjections. This sgRNA tracrRNA contains 2x com stem loops

   Depositor

   Tatjana Sauka-Spengler

   Article

   Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

   Insert

   U6a:2xCom-sgRNA
   

   Expression

   Bacterial

   Promoter

   Zebrafish U6a promoter

   Available Since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pVC-Ds-DrU6a:sgRNA-MS2-Ds

   Plasmid

   #119076

   Purpose

   To clone sgRNA spacer sequence for microinjections. This sgRNA tracrRNA contains 2x MS2 stem loops

   Depositor

   Tatjana Sauka-Spengler

   Article

   Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

   Insert

   U6a:2xMS2-sgRNA
   

   Expression

   Bacterial

   Promoter

   Zebrafish U6a promoter

   Available Since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUA66 PcspA GFP Linker

    Plasmid

    #105604

    Purpose

    Expresses GFP under temperature sensitive promoter PcspA

    Depositor

    Pamela Silver

    Article

    Stirling et al Mol Cell. 2017 Nov 16;68(4):686-697.e3.

    Insert

    GFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PcspA

    Available Since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNB3033

    Plasmid

    #113397

    Purpose

    Ty3 Gag3-His tagged, codon optimized for expression in E. coli; . IPTG inducible

    Depositor

    Suzanne Sandmeyer

    Insert

    Ty3 Gag3 codon optimized for E. coli expression
    

    Tags

    Gag3-6XHis tag

    Expression

    Bacterial

    Available Since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRSFDuet-1_VNp-FGF21

    Plasmid

    #182407

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-fgf21 fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp-fgf21 (FGF21 Human)
    

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKM839

    Plasmid

    #8838

    Depositor

    David Waugh

    Article

    Fox et al FEBS Lett. 2003 Feb 27. 537(1-3):53-7.

    Type

    Empty backbone

    Tags

    P. furiosus MBP

    Expression

    Bacterial

    Available Since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHN873

    Plasmid

    #178829

    Purpose

    Bacterial expression for human Saposin A

    Depositor

    Kazuhiro Abe

    Insert

    artificial gene encoding human Saposin A (AR Human)
    

    Tags

    MBP, His8, TEV

    Expression

    Bacterial

    Mutation

    Codon-usage was optimized for bacterial expressio…

    Promoter

    T7

    Available Since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. ku70 insUP+pPGI+NAT+tNOS+ku70 insD (SBE217)

    Plasmid

    #195049

    Purpose

    integration cassette for ku70 deletion and expression of NAT gene under pPGI for characterization

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    NAT
    

    Expression

    Bacterial

    Promoter

    pPGI

    Available Since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-baPrs-hdNadV

    Plasmid

    #91950

    Purpose

    pET28a-baPrs-hdNadV is a bicistronic vector designed for the simultaneous expression of PRS from Bacillus amyloliquefaciens and NadV from Haemophilus ducreyi

    Depositor

    George Cătălin Marinescu

    Article

    Marinescu et al Sci Rep. 2018 Aug 16;8(1):12278. doi: 10

    Inserts

    Putative Nicotinamide Phosphoribosyl Transferase (nadV Haemophilus ducreyi, strain: ATCC 27722)
    Phosphoribosyl Pyrophosphate Synthetases
    

    Expression

    Bacterial

    Mutation

    changed Leucine 135 to Isoleucine

    Promoter

    T7

    Available Since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pVRa03_1198

    Plasmid

    #49655

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    ECF03_1198
    

    Use

    Synthetic Biology

    Tags

    His6-PreScission

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pT7-lacO

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAC572

    Plasmid

    #127973

    Purpose

    AMA1 plasmid with pyrG selection marker, and USER cassette (PacI/Nt.BbvCI)

    Depositor

    Uffe Mortensen

    Article

    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

    Insert

    pyrG
    

    Use

    Fungal expression

    Expression

    Bacterial

    Promoter

    native promoter

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-EPN-05

    Plasmid

    #79920

    Purpose

    Myr4-12GS-I3-01-myc-Late1

    Depositor

    Neil King

    Article

    Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

    Insert

    EPN-05
    

    Expression

    Bacterial and Mammalian

    Available Since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

20. pCMV-EPN-10

    Plasmid

    #79925

    Purpose

    Palm4-12GS-I3-01-myc-Late1

    Depositor

    Neil King

    Article

    Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

    Insert

    EPN-10
    

    Expression

    Bacterial and Mammalian

    Available Since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only