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pTara:500*

Plasmid

#107954

Purpose

Arabinose-inducible T7*RNAP

Depositor

Jim Haseloff

Article

Boehm et al Nat Commun. 2018 Feb 22;9(1):776. doi: 1

Insert

T7RNAP (modified)

Use

Synthetic Biology

Expression

Bacterial

Mutation

R632S

Available Since

April 2, 2018

Availability

Academic Institutions and Nonprofits only

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pGEM-HE-2xLyn-ERex-Venus-bPAC(F198Y)

Plasmid

#165489

Purpose

Xenopus oocyte expression of PACmn, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activity

Depositor

Thomas Oertner

Article

Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

Insert

2xLyn-ERex-Venus-bPAC(F198Y)

Use

Cdna expression, xenopus oocyte

Tags

Venus

Expression

Bacterial

Promoter

T7

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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MP-P6

Plasmid

#69665

Purpose

Untargetted Bacterial Mutagenesis

Depositor

David Liu

Article

Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

Insert

dnaQ926 dam seqA emrR dinB

Expression

Bacterial

Promoter

E. coli PBAD

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pET28a(+) 6xHis-TEV-IFIT2

Plasmid

#53558

Purpose

bacterial expression of IFIT2

Depositor

Kathleen Collins

Article

Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

Insert

IFIT2 (IFIT2 Human)

Tags

6xHIS and TEV

Expression

Bacterial

Promoter

T7

Available Since

June 3, 2014

Availability

Academic Institutions and Nonprofits only
pET51b-His-TEV-HaloTag7_dead_mt

Plasmid

#167267

Purpose

Overexpression of the dead variant (D106A) of HaloTag7 in E. coli

Depositor

Kai Johnsson

Article

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

HaloTag7

Tags

His x10

Expression

Bacterial

Mutation

D106A

Promoter

T7

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pBW213ara-hrpS

Plasmid

#61435

Purpose

pBAD18-cm encoding rbs33-hrpS-ter

Depositor

Martin Buck, Baojun Wang

Article

Wang et al Nat Commun. 2011 Oct 18;2:508. doi: 10.1

Insert

rbs33-hrpS-ter

Expression

Bacterial

Promoter

pBAD

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pKM061

Plasmid

#134174

Purpose

Bacterial expression of GST-HJURP

Depositor

Iain Cheeseman

Article

McKinley et al Cell. 2014 Jul 17;158(2):397-411. doi: 1

Insert

HJURP (HJURP Human)

Tags

GST

Expression

Bacterial

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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R701-X55-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12V

Plasmid

#159563

Purpose

E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12V

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12V (KRAS Human)

Tags

His6-MBP-tev-Avi

Expression

Bacterial

Mutation

G12V

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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HOXA7 (murine) His-tag pET

Plasmid

#8491

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

Insert

HOXA7 (Hoxa7 Mouse)

Tags

His and T7

Expression

Bacterial

Mutation

Fusion sequence is: ggatccATG; makes protein by …

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
cp193g7

Plasmid

#25950

Depositor

Robert Campbell

Article

Carlson et al Protein Sci. 2010 Aug;19(8):1490-9. doi:

Insert

cp193g7

Tags

His

Expression

Bacterial

Available Since

Sept. 15, 2010

Availability

Academic Institutions and Nonprofits only
pHD_v2

Plasmid

#32183

Depositor

Feng Zhang

Article

Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

Insert

TALE Monomer HD

Expression

Bacterial

Available Since

Oct. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pNG_v2

Plasmid

#32181

Depositor

Feng Zhang

Article

Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

Insert

TALE Monomer NG

Expression

Bacterial

Available Since

Oct. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pBS/KS-mId3sp

Plasmid

#16045

Depositor

Robert Benezra

Article

Jen et al Dev Dyn. 1996 Nov . 207(3):235-52.

Insert

Id3 (Id3 Mouse)

Expression

Bacterial

Mutation

cDNA nucleotides 365-635

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pRG/III-hs-MBP-T43NTR-TrxA-H10

Plasmid

#121677

Purpose

Bacterial expression of neurotensin receptor fusion protein

Depositor

Reinhard Grisshammer, Richard Henderson

Article

Grisshammer et al Protein Expr Purif. 1997 Oct;11(1):53-60

Insert

neurotensin receptor (Ntsr1 Rat)

Tags

TrxA-H10

Expression

Bacterial

Mutation

deleted amino acids 1-42

Promoter

lac

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only
pGEX6P2-MDMX (1-120)

Plasmid

#62065

Purpose

Expression of human MDM2 (1-120) in e. coli

Depositor

Gary Daughdrill

Article

Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

Insert

MDM4 (MDM4 Human)

Tags

GST

Expression

Bacterial

Mutation

contains residues 1-120

Promoter

tac

Available Since

Feb. 5, 2015

Availability

Academic Institutions and Nonprofits only
1xNLS-pMJ915v2

Plasmid

#88915

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Insert

Cas9

Use

CRISPR

Expression

Bacterial

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pT7-agrBD-I

Plasmid

#53439

Purpose

T7-dependent expression of the type-I agrB and agrD genes of S. aureus RN4220

Depositor

Cynthia Collins

Article

Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

Insert

agrBD locus

Use

Synthetic Biology

Tags

V5 epitope, 6x HIS (not expressed on agrB or agrD…

Expression

Bacterial

Mutation

none

Promoter

T7-lac

Available Since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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DNMT3B

Plasmid

#25163

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

DNMT3B_18 (DNMT3B Human)

Tags

His

Expression

Bacterial

Available Since

Aug. 6, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pGEX-4T-1-p19-T7-Vif

Plasmid

#46313

Depositor

Judy Lieberman

Article

Huang et al Nat Biotechnol. 2013 Apr;31(4):350-6. do

Inserts

p19
Vif DNA-1
linker
Vif DNA-2

Use

Pro-sirna

Tags

6XHis and GST

Expression

Bacterial

Available Since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only
pPath-pR-integrase

Plasmid

#200000

Purpose

Entry vector for biosynthetic pathway. pR-phiC31 integrase

Depositor

Farren Isaacs

Article

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

Pathways

Expression

Bacterial

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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