32,967 results
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Plasmid#68298PurposeS2TAG/E17TAG GFP reporterDepositorInsertGFP S2TAG/E17TAG
TagsHisExpressionBacterialAvailable SinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pC235
Plasmid#65952PurposeN1 invertor plasmid for two-strain systemDepositorInsertAiiA
TagsLAA taggedExpressionBacterialPromoterpCin*Available SinceSept. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBALU9
Plasmid#69324PurposeEncodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: bicistronic; FRT-galK-FRT in 2nd GFP intron;use C-term after STOPDepositorInsertgSL2-NLS-GFPint-FgF
ExpressionBacterialAvailable SinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
MS2-ecDHFR
Plasmid#65859PurposeExpresses RNA-binding protein MS2 with eDHFR fusion tagDepositorAvailable SinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTac-E-luci
Plasmid#65937PurposeTheophylline Riboswitch E with luciferase geneDepositorInsertluciferase
ExpressionBacterialAvailable SinceSept. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
BASIC_8_RBS34- mCherry -B0015
Plasmid#68142Purposestorage vector for BASIC part: RBS-RFP(ORF)DepositorInsertRBS34-mcherry-B0015
ExpressionBacterialAvailable SinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRF-AmeR
Plasmid#49356DepositorInsertAmeR repressor
UseSynthetic BiologyExpressionBacterialMutationD120F, R121Q, G123H, Y124T, E125G, M126F, W127YSAvailable SinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc1-FLAGDHFR
Plasmid#63938PurposePosition 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneTagsFLAGExpressionBacterialPromoterT7Available SinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28-MRPP3-myc-His
Plasmid#67864Purposebacterial expression of PRORP (KIAA0391), full coding sequence (mitoch. form) + C-term. myc-His tagDepositorInsertMRPP3 (KIAA0391 Human)
TagsHis and mycExpressionBacterialMutationAmino acid 46 of recombinant MRPP3 is preceded by…PromoterT7Available SinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28-At_PRORP1-His
Plasmid#67869Purposebacterial expression of PRORP1 (At2g32230), full coding sequence (mitoch./chloropl. form) + C-term. His tagDepositorInsertPRORP1 (PRORP1 Mustard Weed)
TagsHisExpressionBacterialMutationResidues 36-572 (see comments)PromoterT7Available SinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28-At_PRORP2-His
Plasmid#67870Purposebacterial expression of PRORP2 (At2g16650), full coding sequence + C-term. His tagDepositorAvailable SinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBW412hrpL-cIgfp
Plasmid#61438PurposepSB4A3 harbouring hrpL-rbs32-cI-lva-ter-Plam-rbs30-gfp-terDepositorInsertHrpL-rbs32-CI-LVA-t-plam-30gfp-t
TagsLVAExpressionBacterialPromoterHrpLAvailable SinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRSET-GST-05230
Plasmid#67874PurposeE.coli expression vector derived from pRSET encoding shy1 (um05230) with a N-terminal GST-fusion.DepositorInsertshy1
TagsGSTExpressionBacterialPromoterT7Available SinceAug. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
234_pSKB3-Venus-LUMP
Plasmid#65975PurposeBacterial expression of Venus-LUMP FRET pairDepositorInsertLUMP
TagsVenusExpressionBacterialPromoterT7Available SinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pVYD
Plasmid#61302PurposeVanillate-inducible gene expression plasmid for Sphingomonas melonis Fr1; destination vector for Gateway cloning, allowing C-terminal fusion of proteins to SYFP2DepositorTypeEmpty backboneTagsSYFP2ExpressionBacterialPromoterPV10Available SinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRSET-spa-GCaMP6f
Plasmid#67563Purposebacterial expression of a photoactivatable fluorescent calcium reporterDepositorInsertspa-GCaMP6f
TagsHIS-tagExpressionBacterialMutationK65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…Available SinceJuly 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pETM6-H9-mCherry
Plasmid#66532PurposeExpresses mCherry under the control of Mutant T7 Promoter H9DepositorInsertmCherry
UseSynthetic BiologyExpressionBacterialMutationCodon Optimized for E. coliPromoterMutant T7 Promoter - H9Available SinceJuly 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pZS1[TreR-T,LacI-T]
Plasmid#60770PurposeContains PI driving expression TreR-T, and PI driving expression of LacI-T.DepositorInsertsTreR-T
Dimeric LacI with the TAN DBD
UseSynthetic BiologyExpressionBacterialMutationDimeric LacI with the TAN DBD. and LacI/GalR repr…Available SinceJuly 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
p148
Plasmid#62616PurposeePathBrick pETM6 vector encoding CsF3H, AaDFR, and DuLARDepositorInsertsflavaone 3B-hydroxylase
dihydroflavonol 4-reductase
leucoanthocyanidin reductase
UseSynthetic BiologyTagsGBD, PDZ, and SH3ExpressionBacterialPromoterT7Available SinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET3a RegIIIg
Plasmid#64936Purposebacterial expression of processed mouse RegIIIgammaDepositorAvailable SinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only