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pET-DEST42-ARL8B-V5-His6

Plasmid

#67425

Purpose

Expression of hArl8b with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Publication

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL8B (ARL8B Human)

Tags

His and V5

Expression

Bacterial

Promoter

T7/lacO

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pET-DEST42-SAR1-V5-His6

Plasmid

#67430

Purpose

Expression of hSar1 with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Publication

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SAR1 (SAR1A Human)

Tags

His and V5

Expression

Bacterial

Promoter

T7/lacO

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pET-DEST42-SARA-V5-His6

Plasmid

#67431

Purpose

Expression of hSara with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Publication

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SARA (SAR1B Human)

Tags

His and V5

Expression

Bacterial

Promoter

T7/lacO

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST14-ARL3

Plasmid

#67376

Purpose

Expression of native hArl3 in E. coli

Depositor

Richard Kahn

Publication

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL3 (ARL3 Human)

Expression

Bacterial

Promoter

T7

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-ARL8A

Plasmid

#67332

Purpose

hArl8a with stop codon in Gateway Entry vector

Depositor

Richard Kahn

Publication

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL8A (ARL8A Human)

Use

Gateway Cloning

Expression

Bacterial

Promoter

None

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-ARL1

Plasmid

#67316

Purpose

hArl1 with stop codon in Gateway Entry vector

Depositor

Richard Kahn

Publication

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL1 (ARL1 Human)

Use

Gateway Cloning

Expression

Bacterial

Mutation

bp 231 C to T; silent mutation

Promoter

None

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
knob-s48i48

Plasmid

#68391

Purpose

Knob protein fused to elastin-like polypeptide (S48I48)

Depositor

Andrew MacKay

Publication

Sun et al J Control Release. 2011 Oct 30;155(2):21

Insert

Knob-S48I48

Tags

Knob

Expression

Bacterial

Promoter

T7

Available since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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MP-U

Plasmid

#69699

Purpose

Untargetted Bacterial Mutagenesis

Depositor

David Liu

Publication

Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

Insert

RBS+ dnaQ926 dam seqA emrR ugi CDA1

Expression

Bacterial

Promoter

E. coli PBAD

Available since

Sept. 23, 2015

Availability

Academic Institutions and Nonprofits only

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GFP S2TAG/E17TAG

Plasmid

#68298

Purpose

S2TAG/E17TAG GFP reporter

Depositor

Jesse Rinehart

Publication

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

GFP S2TAG/E17TAG

Tags

His

Expression

Bacterial

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pC235

Plasmid

#65952

Purpose

N1 invertor plasmid for two-strain system

Depositor

Matthew Bennett

Publication

Chen et al Science. 2015 Aug 28;349(6251):986-9. do

Insert

AiiA

Tags

LAA tagged

Expression

Bacterial

Promoter

pCin*

Available since

Sept. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pBALU9

Plasmid

#69324

Purpose

Encodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: bicistronic; FRT-galK-FRT in 2nd GFP intron;use C-term after STOP

Depositor

Oliver Hobert

Publication

Tursun et al PLoS One. 2009;4(3):e4625. doi: 10.1371/

Insert

gSL2-NLS-GFPint-FgF

Expression

Bacterial

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only
MS2-ecDHFR

Plasmid

#65859

Purpose

Expresses RNA-binding protein MS2 with eDHFR fusion tag

Depositor

Aaron Hoskins

Publication

Carrocci et al Analyst. 2014 Jan 7;139(1):44-7. doi: 10

Inserts

MS2
E. coli Dihydrofolate Reductase (folA )

Tags

HA and NLS

Expression

Bacterial and Yeast

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only
pTac-E-luci

Plasmid

#65937

Purpose

Theophylline Riboswitch E with luciferase gene

Depositor

Justin Gallivan

Publication

Mishler et al Nucleic Acids Res. 2014 Jun;42(10):6753-

Insert

luciferase

Expression

Bacterial

Available since

Sept. 3, 2015

Availability

Academic Institutions and Nonprofits only
BASIC_8_RBS34- mCherry -B0015

Plasmid

#68142

Purpose

storage vector for BASIC part: RBS-RFP(ORF)

Depositor

Geoffrey Baldwin

Publication

Storch et al ACS Synth Biol. 2015 Apr 16.

Insert

RBS34-mcherry-B0015

Expression

Bacterial

Available since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pRF-AmeR

Plasmid

#49356

Depositor

Christopher Voigt

Publication

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

AmeR repressor

Use

Synthetic Biology

Expression

Bacterial

Mutation

D120F, R121Q, G123H, Y124T, E125G, M126F, W127YS

Available since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pST50Trc1-FLAGDHFR

Plasmid

#63938

Purpose

Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

Depositor

Song Tan

Publication

Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

Type

Empty backbone

Tags

FLAG

Expression

Bacterial

Promoter

T7

Available since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pET28-MRPP3-myc-His

Plasmid

#67864

Purpose

bacterial expression of PRORP (KIAA0391), full coding sequence (mitoch. form) + C-term. myc-His tag

Depositor

Walter Rossmanith

Publication

Holzmann et al Cell. 2008 Oct 31;135(3):462-74. doi: 10

Insert

MRPP3 (KIAA0391 Human)

Tags

His and myc

Expression

Bacterial

Mutation

Amino acid 46 of recombinant MRPP3 is preceded by…

Promoter

T7

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pET28-At_PRORP1-His

Plasmid

#67869

Purpose

bacterial expression of PRORP1 (At2g32230), full coding sequence (mitoch./chloropl. form) + C-term. His tag

Depositor

Walter Rossmanith

Publication

Pavlova et al Chembiochem. 2012 Oct 15;13(15):2270-6.

Insert

PRORP1 (PRORP1 Mustard Weed)

Tags

His

Expression

Bacterial

Mutation

Residues 36-572 (see comments)

Promoter

T7

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pET28-At_PRORP2-His

Plasmid

#67870

Purpose

bacterial expression of PRORP2 (At2g16650), full coding sequence + C-term. His tag

Depositor

Walter Rossmanith

Publication

Pavlova et al Chembiochem. 2012 Oct 15;13(15):2270-6.

Insert

PRORP2 (PRORP2 Mustard Weed)

Tags

His

Expression

Bacterial

Promoter

T7

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pBW412hrpL-cIgfp

Plasmid

#61438

Purpose

pSB4A3 harbouring hrpL-rbs32-cI-lva-ter-Plam-rbs30-gfp-ter

Depositor

Martin Buck, Baojun Wang

Publication

Wang et al Nat Commun. 2011 Oct 18;2:508. doi: 10.1

Insert

HrpL-rbs32-CI-LVA-t-plam-30gfp-t

Tags

LVA

Expression

Bacterial

Promoter

HrpL

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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