30,744 results

1. pGEX Smad2 (L+MH2)

   Plasmid

   #15730

   Depositor

   Joan Massague

   Article

   He et al Cell. 2006 Jun 2. 125(5):929-41.

   Insert

   Smad2 (L+MH2) (SMAD2 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   amino acids 187-467 (linker and MH2 regions).

   Available Since

   Sept. 7, 2007

   Availability

   Academic Institutions and Nonprofits only

2. NmeCas9_3XNLS_pMCSG7

   Plasmid

   #120078

   Purpose

   Bacterial expression of wtNmeCas9 with 3X NLS

   Depositor

   Erik Sontheimer

   Article

   Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:

   Insert

   NmeCas9
   

   Expression

   Bacterial

   Available Since

   Feb. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pWKS1583

   Plasmid

   #70188

   Purpose

   Modular vector for secreted amylase reporter expression

   Depositor

   Wiep Klaas Smits

   Article

   Oliveira Paiva et al ACS Synth Biol. 2016 Jun 23.

   Insert

   amyEopt
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   Ptet

   Available Since

   June 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAT222

   Plasmid

   #134890

   Purpose

   Bacterial expression

   Depositor

   Thomas Howard

   Article

   Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   July 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pACYC_HA-ZmSAE1_ZmSAE2-FLAG

   Plasmid

   #91944

   Purpose

   Bacterial expression of maize SAE1 and SAE2

   Depositor

   Richard Vierstra

   Article

   Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

   Inserts

   HA-ZmSAE1
   ZmSAE2-FLAG
   

   Tags

   FLAG and HA

   Expression

   Bacterial

   Available Since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRSF_6His-ZmSUMO1a-GG_ZmSCE1b-Myc

   Plasmid

   #91932

   Purpose

   Bacterial expression of maize SUMO1a and SCE1b

   Depositor

   Richard Vierstra

   Article

   Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

   Inserts

   6His-ZmSUMO1a-GG
   ZmSCE1b-Myc
   

   Tags

   6His and Myc

   Expression

   Bacterial

   Mutation

   Truncation of ZmSUMO1a after the diGlycine motif

   Available Since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFA6a-3GFP-kanMX6

   Plasmid

   #105148

   Purpose

   Yeast gene targeting

   Depositor

   Thomas Pollard, Jian-Qiu Wu, David Kovar

   Article

   Wu et al J Cell Biol. 2006 Jul 31;174(3):391-402.

   Insert

   3GFP-kanMX6
   

   Tags

   3GFP

   Expression

   Bacterial and Yeast

   Available Since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. T7-U5 (long)

   Plasmid

   #111337

   Purpose

   for in vitro snRNA transcription

   Depositor

   Christine Guthrie

   Article

   Ghetti et al RNA. 1995 Apr;1(2):132-45.

   Insert

   snR7-L
   

   Expression

   Bacterial

   Available Since

   Aug. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX-MK1

   Plasmid

   #29638

   Depositor

   Bruce Tempel

   Insert

   Mk1 (Kcna1 Mouse)
   

   Expression

   Bacterial

   Available Since

   May 5, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pET28a(His10-PD-1cyto)

    Plasmid

    #177896

    Purpose

    E coli expression of His10 tag fused to human PD-1 Intracellular Domain

    Depositor

    Ron Vale

    Article

    Hui et al Science. 2017 Mar 31;355(6332):1428-1433

    Insert

    PD-1 (aa 194-288 only) (PDCD1 Human)
    

    Tags

    10xHis-Thrombin cut site-T7 tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMSR:MSMEG_0030:Dendra

    Plasmid

    #159040

    Purpose

    T7 expression of C-term fluorescent fusion in E. coli

    Depositor

    Keith Derbyshire, Joseph Wade

    Article

    Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

    Insert

    MSMEG_0030
    

    Use

    Integrating mycobacterial expression vector attl5

    Tags

    Gly-Ala Linker/Dendra2/1x Flag Tag

    Expression

    Bacterial

    Promoter

    pmycTetO

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. ME1

    Plasmid

    #38857

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    ME1 (ME1 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pSPYngK+-hp

    Plasmid

    #48121

    Purpose

    Shuttle vector E. coli/B.meg.; carrying the codon optimized sequence of the signal peptide for protein secretion of the Bacillus megaterium protein YngK under control of the xylose inducible promoter

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Environ Microbiol. 2010 Jun;76(12):

    Insert

    codon optimized signal peptide of YngK
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBbS5a-RFP

    Plasmid

    #35283

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    lacUV5

    Available Since

    Feb. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. TOPO iRFP670

    Plasmid

    #68443

    Purpose

    TOPO construct expressing iRFP670 for generation of GMAP-compatible gene

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    iRFP670
    

    Use

    Gmap

    Expression

    Bacterial

    Promoter

    Plac

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX6P1-Ubl-sr1(SIRPT1)

    Plasmid

    #75298

    Purpose

    Expresses Sacsin Ubl-sr1 (SIRPT1 repeat) domain (1-339) in fusion with GST (bacterial)

    Depositor

    Kalle Gehring

    Article

    Menade et al J Biol Chem. 2018 Aug 17;293(33):12832-1

    Insert

    Human Sacsin 1-339
    

    Tags

    GST-Tag

    Expression

    Bacterial

    Available Since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMRE167

    Plasmid

    #118515

    Purpose

    broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mClover3
    

    Expression

    Bacterial

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-FLAG-SpCas9-HF1-plus

    Plasmid

    #126775

    Purpose

    Expression of increased fidelity SpCas9-HF1-plus in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as SpCas9-HF1.

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    SpCas9-HF1-plus
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, MBP, and NLS

    Expression

    Bacterial

    Mutation

    N497A; Q695A; Q926A; amino acids 1005-1013 replac…

    Promoter

    T7

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLL1270

    Plasmid

    #129281

    Purpose

    The vector used for enzyme expression in Thermoanaerobacterium. thermosaccharolyticum throughout this study. Contains the CBP promoter from Clostridium thermocellum

    Depositor

    Lee Lynd

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    Clostridium thermocellum DSM1313 Cellobiose Phosp…

    Available Since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. FabF (KASII) pet28 N-terminal His Tag

    Plasmid

    #75017

    Purpose

    Expresses the ketosynthase FabF/KASII from the E. coli fatty acid synthase

    Depositor

    Michael Burkart

    Article

    Worthington et al ACS Chem Biol. 2006 Dec 20;1(11):687-91.

    Insert

    FabF (fabF E. coli)
    

    Tags

    His Tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 21, 2016

    Availability

    Academic Institutions and Nonprofits only