31,059 results

Showing: 11241 - 11260 of 31059 results

Results per page:

Sort results by:

EKAR2G_design1_mTFP_175_Venus_173

Plasmid

#39830

Depositor

Olivier Pertz

Article

Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

Insert

Erk activity reporter

Tags

His-Myc

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
pQTEV-TPT1

Plasmid

#31558

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

tumor protein, translationally-controlled 1 (TPT1 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pmCellFree_KA2_ZIKV C

Plasmid

#145359

Purpose

Cell-free/mammalian expression vector for Zika virus capsid protein with N-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV Capsid

Tags

8xHis and mCherry

Expression

Bacterial

Promoter

T7- CMV/CAG

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5FRT/TO-p97-EQ-mycStrep

Plasmid

#31838

Depositor

Hemmo Meyer

Article

Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

Insert

p97

Tags

Strep and myc

Expression

Bacterial and Mammalian

Mutation

E578Q

Promoter

CMV

Available Since

Oct. 17, 2011

Availability

Academic Institutions and Nonprofits only
pJEC581

Plasmid

#174369

Purpose

RFP reporter used to evaluate CRISPRa in bacteria, contains gRNA target sites every 10 bp upstream of the promoter.

Depositor

James Chappell

Article

Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793

Insert

RFP with PAM rich sequence upstream promoter

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23117

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJEC625

Plasmid

#174368

Purpose

Golden gate vector for gRNA cloning. Contains BbsI sites for insertion of the gRNA spacer as annealed oligos.

Depositor

James Chappell

Article

Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
CHD1 (CHD1A-c107)

Plasmid

#110253

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

CHD1 (CHD1 Human)

Tags

His-TEV

Expression

Bacterial

Promoter

T7 - lacO

Available Since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pdCas9-AsiA

Plasmid

#158063

Purpose

Expressing CasTA of dCas9 fused AsiA

Depositor

Harris Wang

Article

Ho et al Mol Syst Biol. 2020 Jul;16(7):e9427. doi

Insert

dCas9-AsiA

Expression

Bacterial

Promoter

TetO

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-6P-1_WT RAD23A

Plasmid

#201456

Purpose

Expresses human RAD23A with an N-terminal GST tag in E. coli

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog A (RAD23A Human)

Tags

GST

Expression

Bacterial

Mutation

Coding sequence has been optimised for expression…

Promoter

tac promoter

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCA528 SPASTIN , residues 108-200

Plasmid

#180628

Purpose

Bacterial expression for SPASTIN MIT, residues 108-200. Has N-term HIS-SUMO tag. Used for FP experiments. Internal ID: WISP21-10.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

SPASTIN

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 108-200

Promoter

T7

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX2T VPS4B, residues 1-86

Plasmid

#180639

Purpose

Bacterial expression for VPS4B MIT domain, residues 1-86. Has N-terminal GST tag. Internal ID: WISP04-155.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

VPS4B (VPS4B Human)

Tags

GST

Expression

Bacterial

Mutation

Residues 1-86

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pOPINO_F2

Plasmid

#171926

Purpose

Bacterial expression of nanobody VHH_F2, which binds to receptor binding domain of the spike protein of SARS-CoV-2

Depositor

Ray Owens

Article

Huo et al Nat Commun. 2021 Sep 22;12(1):5469. doi:

Insert

VHH_F2

Tags

His6

Expression

Bacterial, Insect, and Mamm…

Available Since

Sept. 23, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
R701-X56-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G13D

Plasmid

#159564

Purpose

E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G13D

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G13D (KRAS Human)

Tags

His6-MBP-tev-Avi

Expression

Bacterial

Mutation

G13D

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
R701-X53-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12C

Plasmid

#159561

Purpose

E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12C

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12C (KRAS Human)

Tags

His6-MBP-tev-Avi

Expression

Bacterial

Mutation

G12C

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pPro18-cm

Plasmid

#17807

Depositor

Jay Keasling

Article

Lee et al Appl Environ Microbiol. 2005 Nov . 71(11

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 28, 2008

Availability

Academic Institutions and Nonprofits only
NAT2

Plasmid

#25182

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

NAT2_07 (NAT2 Human)

Tags

His

Expression

Bacterial

Available Since

Aug. 9, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pET22-HT/prsA

Plasmid

#73624

Purpose

Produces Escherichia coli phosphoribosylpyrophosphate synthetase (PrsA), N-terminal His6 tag

Depositor

T. J. Kappock, James Williamson

Article

Schultheisz et al ACS Chem Biol. 2008 Aug 15;3(8):499-511.

Insert

ribose-phosphate diphosphokinase (prs Escherichia coli str. K-12 substr. MG1655)

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEMEX174-hCALML3

Plasmid

#47600

Depositor

Emanuel Strehler

Article

Qian et al Protein Sci. 1998 Nov;7(11):2421-30.

Insert

CALM3 (CALML3 Human)

Expression

Bacterial

Promoter

T7

Available Since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only
FabF (KASII) pet28 N-terminal His Tag

Plasmid

#75017

Purpose

Expresses the ketosynthase FabF/KASII from the E. coli fatty acid synthase

Depositor

Michael Burkart

Article

Worthington et al ACS Chem Biol. 2006 Dec 20;1(11):687-91.

Insert

FabF (fabF E. coli)

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available Since

May 21, 2016

Availability

Academic Institutions and Nonprofits only
pMUT2-pCaf-mCherry

Plasmid

#192860

Purpose

pMUT2 based vector for sensing caffeine in E. coli Nissle 1917

Depositor

Morten Sommer

Article

Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

Insert

Nanobody-CadC fusion protein and cognate promoter inducible by caffeine

Expression

Bacterial

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge