34,239 results

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pET-28a(+)-His-mEGFP-iLID

Plasmid

#246940

Purpose

Expresses His-mEGFP-iLID in E.coli

Depositor

Makito Miyazaki

Publication

Yamamoto et al Nat Commun. 2025 Aug 26;16(1):7583. doi:

Insert

iLID

Tags

His-tag, mEGFP

Expression

Bacterial

Available since

April 15, 2026

Availability

Academic Institutions and Nonprofits only

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pSJ55 Accessory plasmid with wt T7 DNAP

Plasmid

#253998

Purpose

Accessory plasmid for LySE, carrying wt T7 DNAP for propagation of phage T7∆DNAP

Depositor

Julius Fredens

Publication

Ong et al Nat Microbiol. 2026 May 1. doi: 10.1038/

Insert

T7 DNAP (wt)

Expression

Bacterial

Available since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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TP1320

Plasmid

#252367

Purpose

Expression construct for an ALFA-sfGFP-SUMOEu anti-EMC nanobody with C-terminal His10 tag

Depositor

Tino Pleiner

Publication

Stanton et al bioRxiv [Preprint]. 2026 Jan 15:2026.01.

Insert

anti-EMC nanobody E2

Tags

ALFA-sfGFP-SUMOEu and His10

Expression

Bacterial

Mutation

Contains engineered nanobody variant with ALFA ta…

Available since

April 3, 2026

Availability

Academic Institutions and Nonprofits only

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P23_cymR_Ptec_mCherry

Plasmid

#248113

Purpose

Cumate switch driving mCherry expression

Depositor

Shrikrishnan Sankaran

Publication

Blanch-Asensio et al J Control Release. 2025 Sep 24;387:11426

Inserts

mCherry red fluorescent protein
CymR repressor

Expression

Bacterial

Promoter

P23 and Ptec flanked by cuO operators

Available since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pUCminiTn7-Mariner-sfC-nlp1

Plasmid

#250981

Purpose

pUC mini-Tn7 inserting Mariner transposase-sfCherry-nlp1 into genome

Depositor

Joseph Bondy-Denomy

Publication

Chan et al Cell. 2026 Jul 23;189(15):4810-4824.e7.

Insert

Mariner transposase fused to sfCherry and phage protein Nlp1

Expression

Bacterial

Available since

March 31, 2026

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pUCminiTn7-Mariner-flag

Plasmid

#250982

Purpose

pUC mini-Tn7 inserting Mariner transposase-flag into genome

Depositor

Joseph Bondy-Denomy

Publication

Chan et al Cell. 2026 Jul 23;189(15):4810-4824.e7.

Insert

Mariner transposase with a FLAG tag

Expression

Bacterial

Available since

March 31, 2026

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET15b_LbuCas13a-6xHIS

Plasmid

#251213

Purpose

Bacterial overexpression and recombinant protein purification of LbuCas13a.

Depositor

Niels Geijsen

Publication

Bot et al Commun Biol. 2026 Jan 19;9(1):233. doi:

Insert

LbuCas13a

Tags

HIS

Expression

Bacterial

Promoter

lac

Available since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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JEC1576

Plasmid

#253203

Purpose

pSynORI (Cumate-inducible)

Depositor

James Chappell

Publication

Liu et al Nat Commun. 2026 Feb 2. doi: 10.1038/s41

Type

Empty backbone

Expression

Bacterial

Available since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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JEC1575

Plasmid

#253202

Purpose

pSynORI (IPTG-inducible)

Depositor

James Chappell

Publication

Liu et al Nat Commun. 2026 Feb 2. doi: 10.1038/s41

Type

Empty backbone

Expression

Bacterial

Available since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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JEC1569

Plasmid

#253201

Purpose

pSynORI

Depositor

James Chappell

Publication

Liu et al Nat Commun. 2026 Feb 2. doi: 10.1038/s41

Type

Empty backbone

Expression

Bacterial

Available since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pAJF1131

Plasmid

#252906

Purpose

T7-pelB-NBALFA-LE-6his amp (for purification of his-tagged NBALFA from E. coli)

Depositor

Michael Glickman

Publication

Fay et al mBio. 2025 Aug 13;16(8):e0097125. doi: 1

Insert

NBALFA

Tags

His Tag and pelB leader

Expression

Bacterial

Promoter

T7

Available since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pGLU_446

Plasmid

#250501

Purpose

P12 driving Himar.004 codon harmonized transposase | Barcoded Erythromycin Resistance Cassette Tn Payload | Conjugative Vector

Depositor

Nili Ostrov

Publication

Ortiz et al bioRxiv 2025.12.05.692644

Insert

Himar.004

Use

Synthetic Biology

Expression

Bacterial

Promoter

P12

Available since

March 23, 2026

Availability

Academic Institutions and Nonprofits only

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pGLU_504

Plasmid

#250498

Purpose

Urease Cluster KO | Erythromycin Resistance Cassette | Conjugative Vector

Depositor

Nili Ostrov

Publication

Ortiz et al bioRxiv 2025.12.05.692644

Inserts

ermC
ermC

Use

Synthetic Biology

Expression

Bacterial

Available since

March 23, 2026

Availability

Academic Institutions and Nonprofits only

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pA_higher-Opto_N449S

Plasmid

#253258

Purpose

Opto-CheY N449S expressed using the proA promoter and wild type T7 RBS

Depositor

Aravinthan DT Samuel

Publication

Vrabioiu et al Proc Natl Acad Sci U S A. 2026 Mar 10;12

Insert

Opto-CheY with N449S mutation

Tags

wild type T7 RBS

Expression

Bacterial

Available since

March 19, 2026

Availability

Academic Institutions and Nonprofits only

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pA-Opto_N449S

Plasmid

#253256

Purpose

Opto-CheY N449S expressed using the proA promoter and T7 RBS variant GAAGGAGgT

Depositor

Aravinthan DT Samuel

Publication

Vrabioiu et al Proc Natl Acad Sci U S A. 2026 Mar 10;12

Insert

Opto-CheY with N449S mutation

Tags

T7 RBS variant GAAGGAGgT

Expression

Bacterial

Available since

March 19, 2026

Availability

Academic Institutions and Nonprofits only

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pA-Opto_V416T

Plasmid

#253257

Purpose

Opto-CheY V416T expressed using the proA promoter and T7 RBS variant GAAGGAGgT

Depositor

Aravinthan DT Samuel

Publication

Vrabioiu et al Proc Natl Acad Sci U S A. 2026 Mar 10;12

Insert

Opto-CheY with V416T mutation

Expression

Bacterial

Mutation

T7 RBS variant GAAGGAGgT

Available since

March 19, 2026

Availability

Academic Institutions and Nonprofits only

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V12_ftnA-ftnA

Plasmid

#253338

Purpose

V12 tagged ferritin

Depositor

Qiangjun Zhou

Publication

Luo et al bioRxiv [Preprint]. 2026 Jan 20:2026.01.

Insert

V12-ftnA-ftnA (ftnA E. coli)

Expression

Bacterial

Promoter

T7

Available since

March 19, 2026

Availability

Academic Institutions and Nonprofits only

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scAAV-DDIO-GFP

Plasmid

#251348

Purpose

Cre and Flp dependent DTA (diptheria toxin) expressing system

Depositor

Samuel Pfaff

Publication

Moore et al bioRxiv [Preprint]. 2025 Oct 18:2025.10.

Insert

Cre and Flp dependent eGFP driven by a EF1α(core) promoter

Use

AAV and Cre/Lox

Expression

Bacterial and Mammalian

Promoter

EF1α

Available since

March 19, 2026

Availability

Academic Institutions and Nonprofits only

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pGS1T-PylRSC6a-PylTm15CUA

Plasmid

#251554

Purpose

Production of engineered aaRS/tRNA pair for C6a incorporation, also suitable for C5a and C7a incorporation

Depositor

Alexandria Deliz Liang

Publication

Fischer et al Nat Chem. 2026 Apr 13. doi: 10.1038/s415

Inserts

PylRS-C6a
PylT-m15_CUA

Expression

Bacterial

Promoter

Glutamine synthetase promoter (GlnS) and proK

Available since

March 18, 2026

Availability

Academic Institutions and Nonprofits only

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pRK415-mNeonGreen (constitutive expression)

Plasmid

#248204

Purpose

Constitutive expression of mNeonGreen with a very low copy F-like plasmid under the control of a lac promoter. Plasmid contained within E. coli TOP10 (for cloning)

Depositor

Cecile Fradin

Publication

Sakib et al Methods Appl Fluoresc. 2026 Sep 9;14(4).

Insert

mNeonGreen

Use

Synthetic Biology; Very low copy

Expression

Bacterial

Promoter

lac

Available since

March 18, 2026

Availability

Academic Institutions and Nonprofits only

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