30,410 results

1. pSL103-SpCas9-FLAG-3XNLS

   Plasmid

   #182032

   Purpose

   Expression of recombinant Streptococcus pyogenes Cas9 protein carrying C-terminal FLAG and 3XNLS for genome editing as Cas9 RNP

   Depositor

   Steven Lin

   Article

   Lin et al Bio Protoc. 2022 May 20;12(10):e4420. do

   Insert

   Streptococcus pyogenes cas9 (cas9 Synthetic)
   

   Use

   CRISPR

   Tags

   12X His tag, 3XNLS, FLAG, and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET283xFlagPP7CPHis

   Plasmid

   #28174

   Depositor

   Kathleen Collins

   Insert

   PP7 coat protein optimized
   

   Use

   Tags

   6xhistidine and triple FLAG peptide

   Expression

   Bacterial

   Mutation

   C68A,C73A

   Promoter

   Available Since

   Feb. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-MSP1D1delltaH5

   Plasmid

   #71714

   Purpose

   Membrane scaffold protein (MSP), helix 5 deletion

   Depositor

   Gerhard Wagner

   Article

   Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

   Insert

   Apolipoprotein A1 (APOA1 Human)
   

   Use

   Tags

   6xHis and TEV site

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOPINJ

   Plasmid

   #26045

   Depositor

   Ray Owens

   Article

   Berrow et al Nucleic Acids Res. 2007 . 35(6):e45.

   Insert

   HisGST3C-lacZ
   

   Use

   Tags

   MAHHHHHH_GST_LEVLFQGT

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available Since

   Oct. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pACEC3GH (Hyg)

   Plasmid

   #110084

   Purpose

   Replicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.

   Depositor

   Markus Seeger

   Article

   Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   3C cleavage site - GFP - His10 Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCWU6-xylR-E-luciferase

   Plasmid

   #190721

   Purpose

   fine tune expression of luciferase under the control of xylose- and theophylline- inducible system

   Depositor

   Chenggang Wu

   Article

   Bibek et al bioRxiv. 2023 Apr 24:2023.04.24.538132.

   Insert

   xylR-E-luciferase
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNF02-mLemon

   Plasmid

   #219399

   Purpose

   Constitutive expression of mLemon in E. coli

   Depositor

   Christian Lesterlin

   Article

   Fraikin et al Sci Adv. 2025 Apr 18;11(16):eads6201. do

   Insert

   mLemon
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   proDp

   Available Since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBAD24-sfGFPx1

   Plasmid

   #51558

   Purpose

   Superfolder GFP ORF cloned into pBAD24 for expression in E. coli

   Depositor

   Sankar Adhya, Francisco Malagon

   Article

   Malagon et al RNA. 2013 Sep;19(9):1200-7. doi: 10.1261

   Insert

   superfolder GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJMP1

   Plasmid

   #79873

   Purpose

   Bacillus subtilis dCas9 expression vector; integrates into lacA/ganA

   Depositor

   Jason Peters, Stanley Qi, Carol Gross

   Article

   Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   xylA

   Available Since

   Oct. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAB076i7

    Plasmid

    #79201

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI(RR69)-SH2ABL1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PlacZ-opt (OR1); Ppro1

    Available Since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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