30,733 results

1. pET28a-TmCA-P(11)4

   Plasmid

   #185783

   Purpose

   Encodes thermostable carbonic anhydrase enzyme linked to P(11)4 self-assembling peptide via a GS-linker sequence

   Depositor

   Lizhong He

   Article

   Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

   Insert

   TmCA-P(11)4
   

   Tags

   His-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. UBC53

   Plasmid

   #25471

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   ubc53.001.150 (UBE2U Human)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Aug. 23, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

3. pGST-GstZ1

   Plasmid

   #163090

   Purpose

   Expressing mouse Gstz1 in bacterial cells

   Depositor

   Joseph Takahashi

   Article

   Yang et al Proc Natl Acad Sci U S A. 2019 Oct 29;11

   Inserts

   GstZ1
   GstZ1
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAV10.K5

   Plasmid

   #63210

   Purpose

   pCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI or BciVI).

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Available Since

   Feb. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAV10.K4

   Plasmid

   #63212

   Purpose

   pCACS backbone (Amp), TRP1, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into chrIXR: 387328-388330.

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Available Since

   Feb. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGEM-HE-Venus-bPAC(F198Y)

   Plasmid

   #165487

   Purpose

   Xenopus oocyte expression of soluble photoactivatable adenylyl cyclase derived from bPAC with reduced dark activity

   Depositor

   Thomas Oertner

   Article

   Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

   Insert

   Venus-bPAC(F198Y)-myc
   

   Use

   Cdna expression, xenopus oocyte

   Tags

   Myc and Venus

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHBS1552 H14-SUMO-TDP43 FYW-S-TEV-mCherry

   Plasmid

   #133322

   Purpose

   Bacterial expression of TDP43 with FYW-S mutant CTD

   Depositor

   Rajat Rohatgi

   Article

   Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

   Insert

   TARDBP mutant (TARDBP Human)
   

   Expression

   Bacterial

   Mutation

   All FYW in the TDP43 IDRs mutated to S

   Available Since

   Nov. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHBS1551 H14-SUMO-TDP43 F-S-TEV-mCherry

   Plasmid

   #133321

   Purpose

   Bacterial expression of TDP43 with F-S mutant CTD

   Depositor

   Rajat Rohatgi

   Article

   Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

   Insert

   TARDBP mutant (TARDBP Human)
   

   Expression

   Bacterial

   Mutation

   All F in the TDP43 IDRs mutated to S

   Available Since

   Nov. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pUC-UAS1B16-TEF(203)

   Plasmid

   #44377

   Depositor

   Hal Alper

   Article

   Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

   Insert

   UAS1B16-TEF(203) promoter
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   N/A

   Promoter

   UAS1B16-TEF(203)

   Available Since

   April 16, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pHH0103_SHANK3-1/1

    Plasmid

    #91421

    Purpose

    Protein expression and purification of human SH3 domain construct SHANK3-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    SHANK3-1/1 (SHANK3 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMRE-Tn5-151

    Plasmid

    #118533

    Purpose

    miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mTurquoise2
    

    Use

    Minitn5 delivery vector

    Expression

    Bacterial

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAL-pgi_T

    Plasmid

    #74066

    Purpose

    pAL374 plasmid carrying the pgi (T) sgRNA, targeting the template strand of pgi, SpecR

    Depositor

    Timothy Lu

    Article

    Cleto et al ACS Synth Biol. 2016 Feb 16.

    Insert

    sgRNA pgi (T)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    ptac

    Available Since

    April 12, 2016

    Availability

    Academic Institutions and Nonprofits only

13. NdeI – SpeI(NheI) – BamHI/BglII - ANXA2 (29-339)

    Plasmid

    #136544

    Purpose

    Expresses ANXA2 chimeras in bacterial cells

    Depositor

    Laszlo Nyitray

    Article

    Ecsedi et al Structure. 2020 Aug 4;28(8):943-953.e4.

    Type

    Empty backbone

    Tags

    ANXA2 (29-339) and His-tag

    Expression

    Bacterial

    Available Since

    May 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pED14xY-3

    Plasmid

    #134808

    Purpose

    IPTG inducible expression of Y-TAGA-Evo1 qtRNA

    Depositor

    Ahmed Badran

    Article

    DeBenedictis et al Nat Commun. 2021 Sep 29;12(1):5706. doi:

    Insert

    Y-TAGA-Evo1 qtRNA
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSNAPf-AP

    Plasmid

    #61830

    Purpose

    Encodes SNAP-AP fusion for tagging endogenous target for SNAP-SiMPull

    Depositor

    Aaron Hoskins

    Article

    Rodgers et al RNA. 2015 May;21(5):1031-41. doi: 10.126

    Insert

    SNAP-AP
    

    Use

    Pcr template

    Expression

    Bacterial and Yeast

    Available Since

    April 16, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pMSR:MSMEG_5048:Dendra

    Plasmid

    #167366

    Purpose

    C-term fluorescent fusion

    Depositor

    Joseph Wade, Keith Derbyshire

    Article

    Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

    Insert

    MSMEG_5048
    

    Use

    Integrating mycobacterial expression vector attl5…

    Tags

    Gly-Ala Linker/Dendra2/1x Flag Tag

    Expression

    Bacterial

    Promoter

    pmycTetO

    Available Since

    May 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJMP2824

    Plasmid

    #160671

    Purpose

    Mobile CRISPRi sfGFP 'test', empty sgRNA

    Depositor

    Jason Peters

    Article

    Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

    Insert

    empty sgRNA (BsaI)
    

    Expression

    Bacterial

    Available Since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pETM6-At4CL-CmCHS-CmCHI

    Plasmid

    #73395

    Purpose

    Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

    Depositor

    Mattheos Koffas

    Article

    Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

    Inserts

    At4CL
    CmCHS
    CmCHI
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJL1 Trx-Bx

    Plasmid

    #106290

    Purpose

    Expresses Trx-Bx in Ecoli off a T7 promoter

    Depositor

    James Collins

    Article

    Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

    Insert

    Trx-BX
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET MBP mCerulean LIC cloning vector (MBP-mCerulean)

    Plasmid

    #29751

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    6xHis, AviTag (biotin), MBP, TEV cleavage site, a…

    Expression

    Bacterial

    Available Since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge