33,906 results
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Plasmid#120333PurposeBacterial mCardinal promoter trapDepositorInsertmCardinal
ExpressionBacterialPromoter(None)Available sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO2_Vitiosangium-bGSDM-thrombin
Plasmid#222099PurposeFor expression of a mutant bacterial gasdermin (bGSDM) encoded by a Vitiosangium species with a thrombin protease cleavage site for controlled activation.DepositorInsertVitiosangium-bGSDM-thrombin ORF
Tags6xHis-SUMO2ExpressionBacterialMutationN-terminal methionine deleted and replaced by sin…PromoterT7Available sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO2_Nostoc-bGSDM-WELQ
Plasmid#222100PurposeFor expression of a mutant bacterial gasdermin (bGSDM) encoded by a Nostoc species with a WELQut protease cleavage site for controlled activation.DepositorInsertNostoc-bGSDM-WELQ ORF
Tags6xHis-SUMO2ExpressionBacterialMutationN-terminal methionine deleted and replaced by sin…PromoterT7Available sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDC180
Plasmid#218673PurposeExpresses rat ⍺SNAP in bacteriaDepositorAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAB078d5
Plasmid#79204PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1) luxAB; Ppro1 434cI
ExpressionBacterialPromoterPlacZ-opt (OR1); Ppro1Available sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBV514
Plasmid#204602PurposeExpresses F74A OsTir1-3X Myc from S. cerevisiae ADH1 promoterDepositorInsertOs Tir1 (LOC4335696 Oryza sativa)
TagsMyc epitope tagsExpressionBacterialMutationChanged phenylalanine 74 into an alanine (F74A).PromoterScADH1Available sinceJuly 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-FKBP-empty
Plasmid#221332PurposeProtein overexpression in bacteriaDepositorInsert6xHis-3xFKBP empty scaffold
ExpressionBacterialPromoterT7Available sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMMK805
Plasmid#207139PurposeAssembled Tn10 with ptac promoter driving gfpDepositorInsertTn10-Ptac-GFP
UseSynthetic BiologyExpressionBacterialPromoterptacAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET22b-TEVP-cleavableELP
Plasmid#219402PurposeTobacco etch virus protease-responsive elastin-like polypeptide (ELP)DepositorInsertTEVP-cleavableELP
Tags6xHisExpressionBacterialAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-1-chmI
Plasmid#221341PurposeExpress ChmI in E. coliDepositorInsertchmI
TagsHis6ExpressionBacterialAvailable sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTA-HUH
Plasmid#221152PurposeHisG-URA3-HisG recycling type marker in pTAs for Yarrowia lipolyticaDepositorInsertHisG-URA3-HisG
ExpressionBacterial and YeastAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-tac-EcTyrRS-pAAFRS-9-lpp-tRNA_CUA^EcTyr
Plasmid#218767Purposeencodes EcTyrRS-pAAFRS-9 and EcTyr-tRNA-TAGDepositorInsertEcTyrRS-pAAFRS-9
ExpressionBacterialMutationY37G, L71V, D182C, F183Y, L186C, D265RPromotertacIAvailable sinceJune 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET15-ZmPYK
Plasmid#220230PurposeInducible expression of Zymomonas mobilis pyruvate kinaseDepositorTags6xHis-TEVExpressionBacterialPromoterT7Available sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-hph-Para
Plasmid#218363PurposeEmpty backbone for Tn7-site insertion with araC-ParaBAD expression systemDepositorTypeEmpty backboneExpressionBacterialAvailable sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SUMO-SpyTag003
Plasmid#216282PurposeExpresses SUMO with a C-terminal SpyTag003 in bacterial cellsDepositorInsertSUMO-SpyTag003
TagsHis6 and SpyTag003ExpressionBacterialPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher-⍺-Lock
Plasmid#216286PurposeExpresses DoubleCatcher-⍺-Lock in bacterial cells. DoubleCatcher-⍺-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher ⍺-Lock
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDEST14-DoubleCatcher-β-Lock
Plasmid#216287PurposeExpresses DoubleCatcher-β-Lock in bacterial cells. DoubleCatcher-β-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher β-Lock
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher-γ-Lock
Plasmid#216288PurposeExpresses DoubleCatcher-γ-Lock in bacterial cells. DoubleCatcher-γ-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher γ-Lock
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher-δ-Lock
Plasmid#216289PurposeExpresses DoubleCatcher-δ-Lock in bacterial cells. DoubleCatcher-δ-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher δ-Lock
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher-ε-Lock
Plasmid#216290PurposeExpresses DoubleCatcher-ε-Lock in bacterial cells. DoubleCatcher-ε-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher ε-Lock
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only