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pETNKI-his-3C-LIC-kan

Plasmid

#108703

Purpose

Expression of an His-tagged target protein with 3C protease cleavage site.

Depositor

Titia Sixma

Article

Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

Type

Empty backbone

Tags

His-3C protease cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pBTK563

Plasmid

#110612

Purpose

BTK assembled broad-host-range plasmid encoding NanoLuc luciferase protein driven by CP25 promoter

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

Nanoluc

Expression

Bacterial

Available Since

Aug. 10, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pAT892

Plasmid

#68933

Purpose

Int9 expressed constitutively from PAM4 and the rpiL* RBS, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Insert

Int9

Expression

Bacterial

Available Since

Oct. 2, 2015

Availability

Academic Institutions and Nonprofits only

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sfTq2-C1

Plasmid

#117932

Purpose

Superfolder mTurquoise2

Depositor

Tanneke den Blaauwen

Article

Meiresonne et al Mol Microbiol. 2019 Apr;111(4):1025-1038

Insert

sfTq2

Expression

Bacterial

Available Since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-ChmN

Plasmid

#221348

Purpose

Express ChmN in E. coli

Depositor

Emily Balskus

Article

Crooke et al J Am Chem Soc. 2024 Jun 12;146(23):16268

Insert

chmN

Tags

His6

Expression

Bacterial

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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Pxyl-zapT-mScarlet-I

Plasmid

#207249

Purpose

Integrative plasmid for expressing ZapT-mScarlet-I in Caulobacter crescentus CB15N under Pxyl promoter

Depositor

Suliana Manley

Article

Zhang et al Nat Commun. 2024 Apr 24;15(1):3460. doi:

Insert

ZapT-mScarlet-I

Tags

mScarlet-I

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1 IntS6 AA 1035-1284

Plasmid

#196904

Purpose

Expresses His-tagged Drosophila IntS6 amino acids 1035-1284

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

Integrator subunit 6 (IntS6 Fly)

Tags

6xHis

Expression

Bacterial

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-hs-ALPS 1-ALPS2-mCherry

Plasmid

#187114

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSG00000101199 (ARFGAP1 Human)

Tags

mCherry

Expression

Bacterial

Available Since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pGAT3

Plasmid

#112589

Purpose

Used for expression of protein in E. coli as Thrombin and TEV cleavable N-terminal 6His-tag-GST fusions

Depositor

Marko Hyvönen, Johan Peränen

Article

Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

Type

Empty backbone

Tags

6His-GST

Expression

Bacterial

Available Since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pET29b-ClbPpep-CHis

Plasmid

#48243

Purpose

First 375 amino acids of the peptidase, which contains the soluble periplasmic peptidase domain

Depositor

Emily Balskus

Article

Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

Insert

ClbP-pep

Tags

6-His

Expression

Bacterial

Mutation

First 375 amino acids of the peptidase, which con…

Promoter

T7

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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pBbA7a-RFP

Plasmid

#35316

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

Feb. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pDEST-hPDIb′a′

Plasmid

#60556

Purpose

enhance solubility and correct disulfide bond-formation of fused protein expressed in E.coli

Depositor

Han Choe

Article

Song et al Appl Biochem Biotechnol. 2013 May;170(1)

Type

Empty backbone

Tags

His6-hPDIb'a' tag

Expression

Bacterial

Available Since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pMBA323

Plasmid

#214751

Purpose

To create delta(glmS,nadE) working strains and maintaining their viability

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23105-cas9 + pBAD-λ Red genes + ptet-gRNA (pBR322ori)

Use

CRISPR

Expression

Bacterial

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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Twitch-2C pcDNA3

Plasmid

#104501

Purpose

fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

Insert

Twitch-2C

Expression

Bacterial

Promoter

T7

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pEW4: pET-32a Dot1L(2-386)

Plasmid

#124099

Purpose

Dot1L(2-386) expression plasmid

Depositor

Cynthia Wolberger

Article

Worden et al Cell. 2019 Feb 8. pii: S0092-8674(19)301

Insert

Dot1L(2-386) (DOT1L Human)

Tags

Trx-His-TEV

Expression

Bacterial

Promoter

T7

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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SMARCA2

Plasmid

#73250

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

SMARCA2B (SMARCA2 Human)

Tags

His-6-TEV

Expression

Bacterial

Promoter

T7

Available Since

April 21, 2016

Availability

Academic Institutions and Nonprofits only

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VGFR1_HUMAN_D0

Plasmid

#79743

Purpose

This plasmid encodes the kinase domain of VGFR1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

VGFR1

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pHybr_r2a>m2a(silent)-srt-his

Plasmid

#124807

Purpose

HDR template to knock-in FC silent mIgG2a isotype in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to FC silent producing cell lines.

Depositor

Ferenc Scheeren, Martijn Verdoes

Article

van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

Insert

Murine IgG2a (Fc silent)

Use

CRISPR; Hdr template

Tags

Histag (HHHHHH) and Sortag (LPETGG)

Expression

Bacterial and Mammalian

Mutation

L234A/L235A/N297A

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pHH0103_PACSIN2-1/1

Plasmid

#91330

Purpose

Protein expression and purification of human SH3 domain construct PACSIN2-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

PACSIN2-1/1 (PACSIN2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pMR150

Plasmid

#32292

Depositor

Verne Chapman, Rudolf Jaenisch

Article

Sanford et al Genes Dev. 1987 Dec;1(10):1039-46.

Insert

genomic methylation probe-minor satellite repeat

Expression

Bacterial

Available Since

Jan. 24, 2012

Availability

Academic Institutions and Nonprofits only