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pCashGem-Xyl2-URA3-LINEAR

Plasmid

#174840

Purpose

pRS414 backbone containing LINEAR fragment targeting XYL2 in S. stipitis

Depositor

Zengyi Shao

Article

Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

Insert

Codon optimized Cas9:hGem

Use

CRISPR and Synthetic Biology

Tags

hGeminin tag

Expression

Bacterial and Yeast

Promoter

ENO1p

Available Since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-Pak1 83-149

Plasmid

#12216

Depositor

Jonathan Chernoff

Article

Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

Insert

Pak1 (Pak1 Rat)

Tags

GST

Expression

Bacterial

Mutation

Dominant negative Pak1: autoinhibitory domain, aa…

Available Since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only
pGEX4T1-Smurf2 wt

Plasmid

#13504

Depositor

Jeff Wrana

Article

Kavsak et al Mol Cell. 2000 Dec . 6(6):1365-75.

Insert

SMURF2 (SMURF2 Human)

Tags

GST

Expression

Bacterial

Available Since

Dec. 4, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pTXB1-Tn5-F

Plasmid

#160081

Purpose

Bacterial expression of C-terminally FLAG-tagged Tn5 transposase

Depositor

Pier Pandolfi

Article

Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi

Insert

Tn5 transposase

Tags

FLAG and Mxe intein - Chitin-binding domain

Expression

Bacterial

Available Since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pAG4

Plasmid

#70115

Purpose

Tn7-based lux reporter for promoter cloning in bacteria

Depositor

Thomas Lewis

Article

Glassing et al J Microbiol Methods. 2015 Sep 1;118:75-7

Type

Empty backbone

Expression

Bacterial

Promoter

none

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only
pBXNH3CA

Plasmid

#47069

Purpose

FX cloning E.coli expression vector with araBAD promoter, N-terminal 10x His tag, 3C protease cleavage site and C-terminal Avi-tag

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site), AVI-tag, and…

Expression

Bacterial

Promoter

araBAD

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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MBP-FUS_FL_6E

Plasmid

#98655

Purpose

Expresses MBP-tagged full length phosphomimetic FUS

Depositor

Nicolas Fawzi

Article

Monahan et al EMBO J. 2017 Aug 8. pii: e201696394. doi

Insert

FUS (FUS Human)

Tags

6xHis-MBP

Expression

Bacterial

Mutation

6 S/T to E

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pRG/III-hs-MBP

Plasmid

#121676

Purpose

Bacterial expression of MBP fusion proteins

Depositor

Reinhard Grisshammer, Richard Henderson

Article

Tucker et al Biochem J. 1996 Aug 1;317 ( Pt 3):891-9.

Insert

maltose binding protein

Expression

Bacterial

Promoter

lac promoter

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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OTUD5

Plasmid

#28270

Depositor

Cheryl Arrowsmith

Insert

OTUD5 (OTUD5 Human)

Tags

His tag with TEV cleavage

Expression

Bacterial

Mutation

OTU DOMAIN (residues 172-339).

Available Since

Feb. 10, 2012

Availability

Industry, Academic Institutions, and Nonprofits
pEXP1/Joseph2-Multitagged Plasmid

Plasmid

#226724

Purpose

Validates the expression and detection of epitope-tagged proteins of interest in bacterial cells.

Depositor

Richard Cripps

Article

Robinson et al Biotechniques. 2024;76(7):299-309. doi:

Insert

pEXP1/Joseph2

Tags

6xHis, HA, Myc, GFP, Flag, V5

Expression

Bacterial

Promoter

T7

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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