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Plasmid#78576PurposeOver-expression shuttle vector for gene cloning and transfer from E. coli to Streptomyces. Contains strong constitutive ermEp promoterDepositorTypeEmpty backboneUseStreptomyces expressionTagsExpressionBacterialMutationPromoterermEp* promoter from S. erythraeaAvailable sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only
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pET22b RNase A
Plasmid#58903PurposeBacterial expression of bovine pancreatic ribonuclease ADepositorInsertRNase A (RNASE1 Bovine)
UseTagspelB sequenceExpressionBacterialMutationSee Depositor Comments belowPromoterT7Available sinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET_MBP-RGG-RGG
Plasmid#124946PurposeExpression of MBP-RGG-RGGDepositorInsertMBP-RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
UseTagsHis and MBPExpressionBacterialMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBluescript II SK--POLQ
Plasmid#64873Purposecloning vector for human POLQDepositorInsertPOLQ (POLQ Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
cfSGFP2-anti-AlfaTag nanobody
Plasmid#171818PurposeBacterial expression vector for production of fluorescent anti-AlfaTag nanobodyDepositorInsertcfSGFP2-anti-AlfaTag nanobody
UseTags6xHis and SEP tag (6xD)ExpressionBacterialMutationPromoterAvailable sinceJune 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSNAP-tag (T7) Vector
Plasmid#101137PurposeExpression of protein of interest with SNAP-Tag in bacterial cellsDepositorTypeEmpty backboneUseTagsSNAP-tag (SNAP26b)ExpressionBacterialMutationPromoterAvailable sinceSept. 27, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
MBP-FUS_FL_WT
Plasmid#98651PurposeExpresses MBP-tagged full length FUSDepositorInsertFUS (FUS Human)
UseTags6xHis-MBPExpressionBacterialMutationPromoterAvailable sinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSF-CjPglB
Plasmid#199305PurposepSN18 derivative encoding C.jejuni PglB with C-terminal FLAG epitope tagDepositorInsertCjPglB with flag tag
UseTagsFlag tagExpressionBacterialMutationPromoterpBADAvailable sinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMA7CR_2.0
Plasmid#73950PurposepMA7CR_2.0 contains arabinose inducible λ/RED β-protein and dam, and aTc inducible CRISPR/Cas9 and recXDepositorInsertscas9
lambda beta
dam
recX
UseTagsExpressionBacterialMutationPromoterpAra and pTetAvailable sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNP568
Plasmid#222725PurposepNP555 - IS621 RE-LE TBL-WT, DBL-WT bRNA in cisDepositorInsertIS621 bridge RNA
UseTagssfGFPExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
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tdTomato-anti-AlfaTag nanobody
Plasmid#171819PurposeBacterial expression vector for production of fluorescent anti-AlfaTag nanobodyDepositorInserttdTomato-anti-AlfaTag nanobody
UseTags6xHis and SEP tag (6xD)ExpressionBacterialMutationPromoterAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBAD02_CBASS_Escherichia_coli_SLK172
Plasmid#224395PurposeFor expression of the type I CBASS system encoded by Escherichia coli SLK172DepositorInsertEcCdnD_4TM
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SUMO-DogTag
Plasmid#105629PurposePlasmid for recombinant expression in bacteria of SUMO domain fused to DogTag, permitting covalent linkage to SnoopTagJrDepositorInsertSUMO-DogTag
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE-4
Plasmid#72679PurposeExpresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Cam resistance marker.DepositorInsertmutL E32K
UseTagsnoneExpressionBacterialMutationE32K mutation conferring dominant mutator phenoty…PromoterAvailable sinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pE5n
Plasmid#17459Purposemodified pENTR vector for N-terminal ECFP tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTagsECFPExpressionBacterial, Insect, Mammalia…MutationPromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCas-MmdCas12m-CBE1
Plasmid#192284PurposeMmdCas12m-CBE1 expressed under a constitutive promoterDepositorInsertMmdCas12m-CDA-UGI
UseCRISPRTagsExpressionBacterialMutationD485APromoterPJ23108Available sinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET32a-HD46Q
Plasmid#11515DepositorInserthuntingtin, exon 1, 46Q glutamines (HTT Human)
UseTagsHis, His (out of frame), and TrxExpressionBacterialMutationhuntingtin exon 1 with 46 glutamines, vector m…PromoterAvailable sinceApril 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-CAT
Plasmid#173943PurposeBacterial expression of isolated PARP1 CAT domainDepositorInsertPARP1-CAT (PARP1 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pATetO 6XHis T5 Exonuclease
Plasmid#206209PurposeInducible expression of T5 exonucleaseDepositorInsertT5 exonuclease
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only