32,387 results
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Plasmid#210393PurposeExpress SUMO-GFP in E. coliDepositorInsertGFP
TagsSUMO-tagExpressionBacterialAvailable SinceJan. 13, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pKOV
Plasmid#25769DepositorTypeEmpty backboneExpressionBacterialAvailable SinceAug. 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBSV2G_PflaBS-sgRNA500
Plasmid#149615PurposegRNA expression in B. burgdorferi, parental vectorDepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterPflaBSAvailable SinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNoRBP.iGlucoSnFR2.HaloTag
Plasmid#244105PurposeBacterial expression of green glucose sensor with inert HaloTagDepositorInsertiGlucoSnFR2.HaloTag
TagsHaloTagExpressionBacterialAvailable SinceSept. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNQ071
Plasmid#242082PurposeExpresses E. coli RNAP (gene inserts: rpoA-rpoB-Nter-ybbR-rpoC-6xHis and rpoZ)DepositorInsertrpoA-rpoB-Nter-ybbR-rpoC-6xHis and rpoZ
TagsHis6ExpressionBacterialMutationybbR-peptide tag inserted between E15 and E16 in …Available SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNQ078
Plasmid#242083PurposeExpresses E. coli RNAP (gene inserts: rpoA-rpoB-rpoC-Cter-ybbR--6xHis and rpoZ)DepositorInsertrpoA-rpoB-rpoC-Cter-ybbR-6xHis and rpoZ
TagsHis6ExpressionBacterialMutationybbR-peptide tag inserted between E1377 and G1402…Available SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRECBRR1-Kva1
Plasmid#240703Purposeinverted BRR1 origin of replication plasmid containing Kva1 recombitron with a donor in the ncRNA to target phzM locus in Pseudomonas aeruginosa PAO1 expressed by Pm promoterDepositorInsertKva1 RT,Kva1 ncRNA, PaRecT and PaSSB
ExpressionBacterialMutationInverted BRR1 origin of replicationAvailable SinceSept. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pREC1010-Kva1
Plasmid#240684PurposeRSF1010 origin of replication plasmid containing Kva1 recombitron with a SapI flanked stuffer in the ncRNA expressed by Pm promoterDepositorInsertKva1 RT,Kva1 ncRNA, Beta and EcSSB
ExpressionBacterialMutationWTAvailable SinceSept. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRSF_his6_sumo_SARS_CoV2_NSP14
Plasmid#213518PurposeExpresses NSP14 of SARS-CoV-2DepositorAvailable SinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBSV2G_Psyn-sgRNA500
Plasmid#149614PurposegRNA expression in B. burgdorferi, parental vectorDepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterPsynAvailable SinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDOC-K-glmS-GFPrev
Plasmid#158060PurposeDonor plasmid for insertion of eGFP and the constitutive acpP promoter into the S. Typhimurium chromosome. GFP is in the reverse orientation relative to the kanamycin resistance selectable markerDepositorInsertGreen Fluorescent Protein optimised for excitation with UV light
UseSynthetic BiologyExpressionBacterialPromoteracpPAvailable SinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
4QL1
Plasmid#61942PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertWDR5:APC041-G05:C36415 (WDR5 Human)
Tags6x His and TEV cleavage siteExpressionBacterialMutationSee commentsPromoterT7Available SinceMarch 2, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pIB166
Plasmid#90189PurposeE. coli - Streptococci shuttle plasmid for gene expression in streptococci with P23 promoterDepositorTypeEmpty backboneTagsNoneExpressionBacterialAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-6xHis-MBP-TEV site-TadA8r
Plasmid#208318PurposeOverxpression MBP-TadA8rDepositorInsertTadA8r
ExpressionBacterialMutationmutations in TadA are described in the paperAvailable SinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSL1777 (pEffector)
Plasmid#160731PurposeEffector expression for V. cholerae CAST system, encodes all protein and RNA components. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pCDF backbone.DepositorInsertVchCAST proteins and crRNA
ExpressionBacterialPromoterJ23119Available SinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBLind
Plasmid#113109PurposeVector encoding the RFP gene under blue light-inducible promoter BindF1.DepositorInsertRFP
UseCell-freeExpressionBacterialPromoterBindF1Available SinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQE30-His-CKM
Plasmid#124134PurposeBacterial expression plasmid for PURE system componentsDepositorAvailable SinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET30a-His6-LOXCATmut
Plasmid#140085PurposeA control for His6-LOXCAT where LOX is catalytically dead (mutations H265A and R268A)DepositorInsertA fusion of lactate oxidase from A. viridans and catalase from E. coli
TagsHis6ExpressionBacterialMutationMutation in H265A and R268A in LOX (used original…PromoterT7Available SinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET30a-His6-ABP-LOXCAT
Plasmid#139971PurposeFusion of A. viridans lactate oxidase (LOX) and E. coli catalase (CAT) that converts lactate into pyruvate without producing H2O2. Contains a albumin binding peptide (ABP).DepositorInsertA fusion of lactate oxidase from A. viridans and catalase from E. coli
TagsHis6ExpressionBacterialPromoterT7Available SinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
mt POLRMT
Plasmid#205059PurposePOLRMT protein expressionDepositorAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only