30,416 results

1. pColdI-SBP-eIF4A1

   Plasmid

   #233621

   Purpose

   Expression of SBP-tagged eIF4A1 in E.coli

   Depositor

   Shintaro Iwasaki

   Article

   Chen et al Cell Chem Biol. 2021 Apr 15;28(4):475-48

   Insert

   SBP-eIF4A1 (EIF4A1 Human)
   

   Use

   Tags

   His6-SBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. p3036 GST-HPV-16 E6

   Plasmid

   #10849

   Depositor

   Peter Howley

   Article

   Huibregtse et al EMBO J. 1991 Dec . 10(13):4129-35.

   Insert

   HPV16 E6 (E6 Human papillomavirus)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 24, 2006

   Availability

   Academic Institutions and Nonprofits only

3. CYP51A1 (3I3K)

   Plasmid

   #25161

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   CYP51A1_cw_09 (CYP51A1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Contains amino acid residues 54-502

   Promoter

   Available Since

   Aug. 24, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

4. pAAVS1-TetOn-ZIM3-KRAB-dCas9-P2A-mCherry

   Plasmid

   #212829

   Purpose

   Doxycycline-inducible (Zim3)KRAB-dCas9-HA-P2A-mCherry cassette for integration at the AAVS1-locus of the human genome.

   Depositor

   Wolfgang Enard

   Article

   Edenhofer et al iScience. 2024 May 23;27(6):110090. doi:

   Insert

   ZIM3-KRAB-dCas9-P2A-mCherry
   

   Use

   Tags

   HA-tag and ZIM3-KRAB

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CAG, TetOn

   Available Since

   June 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. MBP-CRY1-PHR

   Plasmid

   #26035

   Depositor

   Johann Deisenhofer

   Article

   Brautigam et al Proc Natl Acad Sci U S A. 2004 Aug 17. 1

   Insert

   CRY1 (CRY1 Mustard Weed)
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 3, 2010

   Availability

   Academic Institutions and Nonprofits only

6. pBHM2329

   Plasmid

   #171686

   Purpose

   pRS316-PCnU6-scaffold, contains template for amplification of PCnU6 and scaffold for fusion PCR

   Depositor

   Hiten Madhani

   Article

   Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi

   Insert

   PCnU6-sgRNA_scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   C. neoformans U6

   Available Since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-MH6-Bsu

   Plasmid

   #163911

   Purpose

   Expresses Bsu large fragment for affinity purification

   Depositor

   Paul Freemont

   Article

   Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

   Insert

   Bsu polymerase
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   March 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. KE-009

   Plasmid

   #133441

   Purpose

   Human elF4H, residue 1-228 (full-length), N-terminal His, GB1, TEV; in pET21 backbone.

   Depositor

   Gerhard Wagner

   Insert

   EIF4H (EIF4H Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. HC078 (3xHA-Atg13)

   Plasmid

   #59544

   Purpose

   Expresses yeast Atg13 under the endogenous promoter with a 3xHA N-terminal tag

   Depositor

   Daniel Klionsky

   Article

   Cheong et al Mol Biol Cell. 2008 Feb;19(2):668-81. Ep

   Insert

   ATG13 (ATG13 Budding Yeast)
   

   Use

   Tags

   3xHA

   Expression

   Bacterial and Yeast

   Mutation

   AvrII site created after start codon

   Promoter

   ATG13

   Available Since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pttq18

    Plasmid

    #69122

    Purpose

    regulated expression of genes in Escherichia coli

    Depositor

    Michael Stark

    Article

    Stark et al Gene. 1987;51(2-3):255-67.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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