30,416 results

1. pBAV1K-T5-gfp

   Plasmid

   #26702

   Depositor

   Ichiro Matsumura

   Article

   Bryksin et al PLoS One. 2010 . 5(10):e13244.

   Insert

   T5lacOlacOeGFP biobrick
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 15, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPET-PKR/PPase

   Plasmid

   #42934

   Depositor

   James Cole

   Article

   Lemaire et al J Mol Biol. 2005 Jan 7;345(1):81-90.

   Inserts

   protein kinase R (EIF2AK2 Human)
   phage lambda phosphatase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   April 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pColdI-SBP-eIF4A1

   Plasmid

   #233621

   Purpose

   Expression of SBP-tagged eIF4A1 in E.coli

   Depositor

   Shintaro Iwasaki

   Article

   Chen et al Cell Chem Biol. 2021 Apr 15;28(4):475-48

   Insert

   SBP-eIF4A1 (EIF4A1 Human)
   

   Use

   Tags

   His6-SBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pQF

   Plasmid

   #48095

   Purpose

   Cumate-inducible expression of 3xFLAG-tagged fusion protein

   Depositor

   Julia Vorholt

   Article

   Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

   Type

   Empty backbone

   Use

   Tags

   3xFLAG

   Expression

   Bacterial

   Mutation

   Promoter

   PQ5

   Available Since

   Dec. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTn7CdrA-gfp(ASV)C

   Plasmid

   #111617

   Purpose

   Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species.

   Depositor

   Tim Tolker-Nielsen

   Article

   Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

   Insert

   PcdrA-RBSII-gfp(ASV)-T0-T1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET His6 GST TEV LIC cloning vector (1G)

   Plasmid

   #29655

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   6xHis, GST, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available Since

   June 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET15-UbE2D1

   Plasmid

   #15782

   Depositor

   Wade Harper

   Article

   Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

   Insert

   Ubiquitin conjugating enzyme UbE2D1 (UBE2D1 Human)
   

   Use

   Tags

   6x His, T7 tag, and Xpress tag (enterokinase clea…

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDD120

   Plasmid

   #119879

   Purpose

   Constitutive expression of Che9c60 and Che9c61 recombinases on pB264/pBR322 backbone with kanamycin resistance.

   Depositor

   Tae Seok Moon

   Article

   DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

   Insert

   pConstitutive_Che9c60 and Che9c61
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSET-mSA

   Plasmid

   #39860

   Depositor

   Sheldon Park

   Article

   Lim et al Biotechnol Bioeng. 2013 Jan;110(1):57-67

   Insert

   monomeric streptavidin
   

   Use

   Tags

   6xHis and FLAG

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-dCas9-VP64-6xHis

    Plasmid

    #62935

    Purpose

    Expression of dCas9-VP64-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    dCas9-VP64
    

    Use

    Tags

    3xFLAG, 6xHis, NLS, and VP64

    Expression

    Bacterial

    Mutation

    D10A and H840A amino acid changes render Cas9 nuc…

    Promoter

    T7

    Available Since

    May 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge