32,295 results
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Plasmid#211804PurposeExpresses inactive 2',3'-cyclic nucleotide phosphodiesterase catalytic domain (catalytic residues mutated)DepositorInsert2',3'-cyclic nucleotide phosphodiesterase catalytic domain (Cnp Rat)
ExpressionBacterialMutationH73L H153LPromotertetAvailable SinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pK19pA-MN
Plasmid#86973PurposeChIC; genomic mapping of chromatin proteinsDepositorInsertprotein_A fused to Staphylococcus aureus microccocal nuclease (MNase)
ExpressionBacterialAvailable SinceMarch 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSAB35
Plasmid#175787PurposeExpresses Legionella pneumophila SidK (1-278) with a cleavable N-terminal His tag (TEV site) and C-terminal 3xFLAG tagDepositorInsertSidK (1-278)
Tags3xFLAG and 6xHis TEVExpressionBacterialAvailable SinceDec. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVoPo-04
Plasmid#187839PurposeSuicide vector to generate markerless gene deletions in Fusobacterium nucleatumDepositorTypeEmpty backboneExpressionBacterialAvailable SinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
ILF2_DZF_pGEX
Plasmid#135162PurposeRecombinant expression of ILF2 with dual-affinity tagDepositorAvailable SinceNov. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
Human_TFAM_NoMTS_pET28
Plasmid#34705DepositorInsertTFAM (TFAM Human)
Tags6XHisExpressionBacterialMutationEncodes residues 43–246, corresponding to full-le…PromoterT7Available SinceFeb. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet-1_Ec_rpoZ
Plasmid#128837PurposeE. coli rpoz gene cloned between NcoI and HindIII sites of the first MCS of this Duet plasmid. Omega protein.DepositorAvailable SinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28b-ULP1-405-621
Plasmid#229224PurposeFor expression of ULP1 proteinDepositorAvailable SinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
Luciferase
Plasmid#213979PurposeExpresses Doxy Inducible LuciferaseDepositorInsertLuciferase
UseLentiviralTags6xHis affinity tagExpressionBacterial and MammalianPromoterTet-responsive promoter PTight, consisting of sev…Available SinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCIOX
Plasmid#51300PurposeE.coli expression vector for His8-SUMO tagged recombinant proteinDepositorTypeEmpty backboneTagsHis8 + SUMOExpressionBacterialPromoterT7Available SinceFeb. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET23b-15F11-HA-mEGFP-6xHis
Plasmid#129593PurposeThe encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.DepositorInsertAnti-HA frankenbody-mEGFP (15F11-HA scFv-mEGFP)
Tags6xHis-tagExpressionBacterialPromoterT7Available SinceAug. 15, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET24a isPETase
Plasmid#225759PurposeExpresses codon-optimized PETase from Ideonella sakaiensis in E. coli without native signal sequence and with a C-terminal his-tagDepositorInsertisPETase
Tags6xHis TagExpressionBacterialPromoterT7Available SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
M13KO7-ΔPS-ΔgeneIII
Plasmid#134351PurposeHelper phage for M13 phage productionDepositorInsertHelper phage gene
ExpressionBacterialAvailable SinceJune 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
GST PTBP1 isoform 4
Plasmid#108594PurposeRecombinant GST tagged protein productionDepositorInsertPTBP1 isoform 4 (PTBP1 Human)
TagsGSTExpressionBacterialMutationisoform 4 (isoform a)PromotertacAvailable SinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSB1A3 EcoRI-RTX with EcoRI Methylase-AmilCP
Plasmid#85165PurposeProvides the coding region to express the EcoRI restriction enzyme. The RTX tag allows the EcoRI enzyme to be isolated via calcium-precipitation.DepositorInsertEcoRI-RTX and EcoRI Methylase-AmilCP coding sequences
TagsAmilCP and RTXExpressionBacterialPromoterJ23100 promoterAvailable SinceDec. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTP1174
Plasmid#104162PurposeBacterial expression plasmid of anti-mouse kappa chain nanobody TP1170 (1x Cysteine)DepositorInsertAnti-mouse kappa chain specific nanobody TP1170 (1xCysteine)
Tags14xHistidine tag and NEDD8 from Brachypodium dist…ExpressionBacterialAvailable SinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHN781
Plasmid#178828PurposeBacterial expression for human Saposin ADepositorInsertartificial gene encoding human Saposin A (AR Human)
TagsHis8, TEVExpressionBacterialMutationCodon-usage was optimized for bacterial expressio…PromoterT7Available SinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCASB
Plasmid#190175PurposePlasmid for yeast expression of Cas9; contains a golden gate cloning site to additionally express a gRNA for a target sequenceDepositorInsertsgRNA cloning site
ExpressionBacterial and YeastMutationAdded 5'-agagacc-3' upstream and 5'…Available SinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
Mouse ZP3 promoter (JD#445)
Plasmid#14634DepositorInsertZP3 promoter (Zp3 Mouse)
ExpressionBacterialAvailable SinceMarch 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
pTW211b5
Plasmid#136927PurposeDirected evolution of SpCas9DepositorInsertCas9(N)-NpuN
UseCRISPRExpressionBacterialMutationsee manuscriptAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only