32,218 results
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Plasmid#60721PurposeExpresses the N-terminal fragment of split T7 RNAP split a position 179. Insert is driven by PBAD. Contains a constitutive araC ORFDepositorInsertResidues 1-179 of T7 RNAP
UseSynthetic BiologyExpressionBacterialMutationOnly amino acids 1-179 of T7 RNAPPromoterPBADAvailable SinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHS1928
Plasmid#205967PurposeJAGBLH010000220 Cas8-HNH operon (E. coli codon optimized) with His-tagged Cas8 in pET45b(+)DepositorInsertCas8-HNH all proteins, with His-tagged Cas8
Tags14xHis on Cas8ExpressionBacterialPromoterT7Available SinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
B3-2
Plasmid#111472Purposetranslational elongation factor 4DepositorInserttranslational elongation factor 4
Tags6xHisExpressionBacterialAvailable SinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTrc99S-ssDsbA-MBP-4xDQNAT
Plasmid#128398PurposePeriplasmic expression of E. coli MBP with C terminal 4xDQNAT glycosylation sites in E. coliDepositorInsertMaltose binding protein
Tags4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…ExpressionBacterialMutationY342H- please see depositor commentsPromotertrcAvailable SinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET_StrepII_TEV_LIC_TniQ(PmcCAST)
Plasmid#224927PurposeTniQ expression plasmid for the co-purification of TnsC-TniQ complexDepositorInsertTniQ
UseCRISPRExpressionBacterialAvailable SinceApril 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD80RFP-pXD68Kan2-RFP
Plasmid#225329PurposeRSF1010-based broad host-range plasmid engineered from pXD68Kan2 encoding RFP, kanamycin resistanceDepositorInsertRFP
ExpressionBacterialAvailable SinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
QUAS-5-T7-TetO-GFP_ColE1
Plasmid#171660PurposeQUAS five base pairs upstream of a T7 promoter with a TetO operator. Expresses GFP in BL21(DE3) E. coli according to the Q and Tet systems. Contains the ColE1 origin of replication.DepositorInsertQUAS-5-T7-TetO-GFP ssra-T7 Stop
TagsssrA degradation tag (DAS+4)ExpressionBacterialPromoterT7Available SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
T7-LacO-QF-T7-TetO-TetR_p15A
Plasmid#171661PurposeExpresses QF and/or TetR in BL21(DE3) E. coli according to the presence of aTc. There is no T7 terminator sequence between the QF- and TetR-containing circuits. Contains a p15A origin of replication.DepositorInsertQF-T7-TetO-TetR
ExpressionBacterialPromoterT7Available SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-NTOM20-mVenus-Zdk3
Plasmid#81012PurposeExpression of Zdk1 anchored to mitochondriaDepositorInsertZdk3
Tags6x His tag, FLAG, NTOM20, and mVenusExpressionBacterial, Insect, and Mamm…Available SinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
DUSP13
Plasmid#38824PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only