30,008 results

1. pCIRCgp41-1

   Plasmid

   #74227

   Purpose

   Vector for SICLOPPS-mediated protein circularization using the gp41-1 intein.

   Depositor

   Barbara Di Ventura, Roland Eils

   Article

   Waldhauer et al Mol Biosyst. 2015 Nov 10;11(12):3231-43.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   gp41-1 C-intein and gp41-1 N-intein

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lac

   Available since

   April 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNH-TrxT

   Plasmid

   #26106

   Purpose

   SGC Empty backbone for bacterial expression

   Depositor

   Opher Gileadi

   Article

   Savitsky et al J Struct Biol. 2010 Jun 10. ():.

   Type

   Empty backbone

   Use

   Tags

   6xHis, TEV cleavage site, and TrxA

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available since

   Dec. 2, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET30a-AlkB-D135S

   Plasmid

   #79051

   Purpose

   Express mutant AlkB protein (D135S) in E. coli BL21(DE3)

   Depositor

   Tao Pan

   Article

   Zheng et al Nat Methods. 2015 Sep;12(9):835-7. doi:

   Insert

   AlkB (alkB E.coli)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   A truncated AlkB with deletion of the amino (N)-t…

   Promoter

   T7

   Available since

   Aug. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. spNW25

   Plasmid

   #173484

   Purpose

   Expresses spNW25 in Bl21

   Depositor

   Huan Bao

   Article

   Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

   Insert

   spNW25
   

   Use

   Tags

   Histag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNHEJ-recX-sacB

   Plasmid

   #158718

   Purpose

   CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses NHEJ machinery of M. marinum and recX.

   Depositor

   Yi-Cheng Sun

   Article

   Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

   Insert

   recX
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pmyc

   Available since

   Jan. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. PBAD-his6-prkA-pACYC184

   Plasmid

   #41041

   Depositor

   Ichiro Matsumura

   Article

   Parikh et al Protein Eng Des Sel. 2006 Mar;19(3):113-

   Insert

   prkA
   

   Use

   Tags

   6xHis and T7 epitope tag

   Expression

   Bacterial

   Mutation

   Promoter

   PBAD

   Available since

   Nov. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. KARS_pNIC-Bio3

   Plasmid

   #153051

   Purpose

   Expression construct for producing Avi tagged Lysyl-tRNA synthetase

   Depositor

   Susanne Gräslund

   Insert

   KARS (KARS1 Human)
   

   Use

   Tags

   Avi and His6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pREDusk-MCS

   Plasmid

   #188970

   Purpose

   For red light-repressed bacterial expression of gene of interest

   Depositor

   Heikki Takala

   Article

   Multamaki et al ACS Synth Biol. 2022 Aug 23. doi: 10.102

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pC0059 PbuCas13 (B03) His6-TwinStrep-SUMO-BsaI

   Plasmid

   #115209

   Purpose

   Expresses PbuCas13b from a SUMO-tagged bacterial expression construct

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

   Insert

   PbuCas13
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-TK-Firefly-Control

    Plasmid

    #122277

    Purpose

    Lentiviral vector for stable expression of Firefly luciferase.

    Depositor

    Demián Cazalla

    Article

    Gorbea et al Elife. 2019 Sep 20;8. pii: 50530. doi: 1

    Insert

    Firefly luciferase
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Thymidine kinase

    Available since

    April 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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