34,175 results

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HUWE1

Plasmid

#25211

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

huwe1.4005.4374 (HUWE1 Human)

Tags

His

Expression

Bacterial

Mutation

HECT domain

Available since

Feb. 18, 2011

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article
PERK 4: PERKKD-pGEX4T-1

Plasmid

#21817

Depositor

David Ron

Publication

Harding et al Nature. 1999 Jan 21. 397(6716):271-4.

Insert

PERK (537-1114) (Eif2ak3 Mouse)

Tags

GST and Myc (9E10)

Expression

Bacterial

Mutation

PERK Kinase Domain. Amino acids 537-1114.

Available since

March 29, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pSEL1

Plasmid

#23011

Depositor

James Bowie

Publication

Massey-Gendel et al Protein Sci. 2009 Feb . 18(2):372-83.

Insert

DHFR (Dhfr Mouse)

Tags

6 His Tag (not in frame) and Xpress epitope (not …

Expression

Bacterial

Available since

March 10, 2010

Availability

Academic Institutions and Nonprofits only
pBAD33.lon

Plasmid

#22145

Depositor

Robert Sauer

Publication

Gur et al Genes Dev. 2008 Aug 15. 22(16):2267-77.

Insert

Lon (lon E. coli K12)

Expression

Bacterial

Mutation

None, WT gene

Available since

Sept. 28, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBS mNFATc3-EE

Plasmid

#17868

Depositor

Jerry Crabtree

Publication

Graef et al Cell. 2001 Jun 29. 105(7):863-75.

Insert

NFATc3 (Nfatc3 Mouse)

Tags

EE

Expression

Bacterial

Available since

April 29, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pDEST17-UbE2H

Plasmid

#15752

Depositor

Wade Harper

Publication

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2H (UBE2H Human)

Tags

His

Expression

Bacterial

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1 EGFP-Sumo2

Plasmid

#13385

Depositor

Mary Dasso

Publication

Azuma et al J Cell Biol. 2003 Nov 10. 163(3):477-87.

Insert

Sumo2 (SUMO2 Human)

Tags

EGFP and GST

Expression

Bacterial

Available since

Nov. 16, 2006

Availability

Academic Institutions and Nonprofits only
GEV 1

Plasmid

#12615

Depositor

John Louis

Publication

Huth et al Protein Sci. 1997 Nov . 6(11):2359-64.

Insert

GB1 domain of protein G as N terminal tag, with Bcl2 as insert (Bcl2 Mouse)

Tags

G protein and His

Expression

Bacterial

Available since

Sept. 22, 2006

Availability

Academic Institutions and Nonprofits only

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pET32a-HD25Q

Plasmid

#11508

Depositor

Pamela Bjorkman

Publication

Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

Insert

huntingtin, exon 1, 25 glutamines (HTT Human)

Tags

His, His (out of frame), and Trx

Expression

Bacterial

Mutation

huntingtin exon 1 with 25 glutamines, vector m…

Available since

April 28, 2006

Availability

Academic Institutions and Nonprofits only

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Cys-3HB-SPYcatcher

Plasmid

#214838

Purpose

This plasmid encodes a protein (Cys-3HB-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

Depositor

Hironori Funabiki

Publication

Arimura et al eLife. 2025 May 20;13:RP103486. doi: 10.

Insert

Cys-3HB-SPYcatcher

Tags

His-TEV-SUMO-

Expression

Bacterial

Promoter

T5

Available since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET-PfuX7

Plasmid

#182364

Purpose

Expresses PfuX7 DNA polymerase

Depositor

Morten Norholm

Publication

Norholm et al BMC Biotechnol. 2010 Mar 16;10:21. doi:

Insert

PfuX7 DNA polymerase

Tags

His-tag

Expression

Bacterial

Mutation

V93Q

Promoter

T7

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pNeg-Ma-barnase-TAG3-TAG45

Plasmid

#197572

Purpose

Negative selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses 2xTAG-codon interrupted barnase gene and M. alvus Pyl-tRNA(6). p15a origin of replication.

Depositor

Ryan Mehl

Publication

Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

Inserts

Barnase - 2xTAG
M. alvus Pyl-tRNA (6)

Tags

none

Expression

Bacterial

Mutation

3TAG and 45TAG

Promoter

araC and lpp

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pET-Neq2X7

Plasmid

#182366

Purpose

Expresses Neq2X7 DNA polymerase

Depositor

Morten Norholm

Publication

Hernandez-Rollan et al BMC Biotechnol. 2024 Apr 2;24(1):17. doi

Insert

Neq2X7 DNA polymerase

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available since

Jan. 25, 2023

Availability

Academic Institutions and Nonprofits only

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2Bc-T MBP_BoMoC(ed)_6xH

Plasmid

#186461

Purpose

Recombinant B. mori truncated R2 RT expression

Depositor

Kathleen Collins

Publication

Upton et al Proc Natl Acad Sci U S A. 2021 Oct 19;11

Insert

B. mori truncated R2 RT

Tags

6xHis and MBP

Expression

Bacterial

Promoter

T7

Available since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET28a-MH6-uvsY

Plasmid

#163914

Purpose

Expresses uvsY for bacterial expression and affinity purification

Depositor

Paul Freemont

Publication

Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

Insert

uvsY (uvsY Enterobacteria phage T4)

Tags

His

Expression

Bacterial

Promoter

T7 promoter

Available since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST14-DogCatcher

Plasmid

#171772

Purpose

Expresses DogCatcher protein in bacterial cytoplasm

Depositor

Mark Howarth

Publication

Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

Insert

DogCatcher

Tags

His6 and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pGEX-4T1 human PP2A Aα full length

Plasmid

#132634

Purpose

expresses human PP2A Aα full length in E. coli

Depositor

Wenqing Xu

Publication

Sangodkar et al J Clin Invest. 2017 Jun 1;127(6):2081-20

Insert

PP2A Aα (PPP2R1A Human)

Expression

Bacterial

Available since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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SpyCatcher002-oDi

Plasmid

#124661

Purpose

Expresses SpyCatcher002-oDi for dimerization of SpyTag-proteins via dimeric coiled-coil

Depositor

Mark Howarth

Publication

Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

Insert

SpyCatcher002-oDi

Tags

C-tag, His6, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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phage UbiC G3BP1-SNAP

Plasmid

#119949

Purpose

Lentiviral vector expressing G3BP1-SNAP fusion protein

Depositor

Jeffrey Chao

Publication

Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

G3BP1 (G3BP1 Human)

Use

Lentiviral

Tags

SNAP

Expression

Bacterial and Mammalian

Promoter

UbiC

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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sfTq2ox-PBP5

Plasmid

#117960

Purpose

Periplasmic expression of superfolder mTurquoise2ox (pNM077 pTHV037-DsbAss-LEGPAGL-sfTq2ox-EFGS-∆1-17-PBP5)

Depositor

Tanneke den Blaauwen

Publication

Meiresonne et al Mol Microbiol. 2019 Apr;111(4):1025-1038

Insert

sfTq2ox-PBP5 (dacA )

Expression

Bacterial

Available since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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