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34,175 results

Showing: 1781 - 1800 of 34175 results

  2. pET-21b(+)-Is-PETase-W185A

    Plasmid
    #112204
    Purpose
    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W185A mutations, codon optimized for expression in E. coli K12
    Depositor
    Gregg Beckham, Christopher Johnson
    Publication
    Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115
    Insert
    PETase gene from Ideonella sakaiensis 201-F6 with W185A mutation, codon optimized for expression in E. coli K12
    Tags
    6XHIS
    Expression
    Bacterial
    Mutation
    W185A
    Promoter
    N/A
    Available since
    July 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    112204-plasmid-map-sequence-id-215853

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  11. eSrtA(4S-9) pET29b

    Plasmid
    #75146
    Purpose
    reprogrammed sortase A variant that recognizes alternative substrate LPXSG with high activity and specificity
    Depositor
    David Liu
    Publication
    Dorr et al Proc Natl Acad Sci U S A. 2014 Sep 16;11
    Insert
    reprogrammed sortase A variant
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    N98D S102C A104V A118T F122A K134R F144L I182V E1…
    Promoter
    T7
    Available since
    Aug. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    75146-plasmid-map-sequence-id-142065

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  14. pET22b_PstS_3

    Plasmid
    #78200
    Purpose
    Expression of double-cysteine variant of mature phosphate binding protein, PstS, which also has a mutation to weaken phosphate binding, as scaffold for phosphate biosensor
    Depositor
    Martin Webb
    Publication
    Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62
    Insert
    pstS (A17C,I76G,A197C)
    Expression
    Bacterial
    Mutation
    A17C,I76G,A197C (please see depositor comment bel…
    Promoter
    T7
    Available since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    78200-plasmid-map-sequence-id-146050

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  17. pET21 MBPx-SpyCatcher

    Plasmid
    #72327
    Purpose
    Bacterial expression of tandem high affinity maltose binding protein domains linked to SpyCatcher, for solid-phase polyprotein synthesis.
    Depositor
    Mark Howarth
    Publication
    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p
    Insert
    pET21 MBPx-SpyCatcher
    Tags
    N-terminal His6 tag
    Expression
    Bacterial
    Mutation
    MBP contains A312V, I317V mutations and 172, 173,…
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    72327-plasmid-map-sequence-id-434061

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  19. PGR-Blue

    Plasmid
    #68374
    Purpose
    Terminator quantification vector utilizing Golden Gate Assembly and amilCP. Positive colonies are green and false positives are blue. Terminators are placed between GFP and RFP - induced by arabinose.
    Depositor
    Nathan Reyna
    Type
    Empty backbone
    Use
    Synthetic Biology; Golden gate assembly
    Expression
    Bacterial
    Promoter
    pBAD
    Available since
    Oct. 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    68374-plasmid-map-sequence-id-121945

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Showing: 1781 - 1800 of 34175 results