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pGEX iLOV

Plasmid

#26587

Depositor

John Christie

Article

Chapman et al Proc Natl Acad Sci U S A. 2008 Dec 16. 1

Insert

iLOV

Tags

GST

Expression

Bacterial

Available Since

Dec. 2, 2010

Availability

Academic Institutions and Nonprofits only

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M13-kmR

Plasmid

#206861

Purpose

M13KE from NEB modified with kanamycin resistance gene and NotI restriction site on g3p ( M13KE-NotI-Kan)

Depositor

Irene Chen

Article

Lin et al PLoS One. 2011;6(5):e19991. doi: 10.1371

Insert

Kanamycin resistance gene

Expression

Bacterial

Promoter

lac

Available Since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAG87 FAST

Plasmid

#130719

Purpose

Expresses His-tagged FAST (also called YFAST) in bacteria

Depositor

Arnaud Gautier

Article

Plamont et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

Insert

FAST

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pRAM24

Plasmid

#234313

Purpose

pBBR1 origin cat-RNA U1376

Depositor

James Chappell

Article

Kalvapalle et al Nat Biotechnol. 2025 Mar 18. doi: 10.103

Insert

U1367 Targeting RAM, Barcode 1

Use

Synthetic Biology

Expression

Bacterial

Promoter

P. CymRC

Available Since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

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pAJM.677

Plasmid

#108530

Purpose

AraCAM + AraE + PBAD-YFP Reporter. Ara sensor for use in non-Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

AraCAM + AraE + PBAD-YFP

Expression

Bacterial

Mutation

AraC: L133F E165L E169G C280L

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pComb3XTT

Plasmid

#63891

Purpose

Backbone of pComb3X containing TT Fab (light and heavy chains) against tetanus toxin, used as phage display/expression control and PCR template for human antibody constant regions (kappa and CH1)

Depositor

Carlos Barbas

Article

Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):

Insert

TT Fab

Use

Phage display

Tags

6xHis, HA tag, and geneIII

Expression

Bacterial

Promoter

lacZ

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pChillyBoys_gen1

Plasmid

#228495

Purpose

pACYCDuet-1 encoding Oleispira antarctica GroEL and GorES low temp chaperonins for enhanced low-temperature induction (E. coli based protein expression)

Depositor

Richard Baker

Inserts

groEL
groES

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pTARA

Plasmid

#31491

Depositor

Kathleen Matthews

Article

Wycuff et al Anal Biochem. 2000 Jan 1. 277(1):67-73.

Insert

RNA polymerase

Expression

Bacterial

Mutation

N823D

Available Since

June 22, 2012

Availability

Academic Institutions and Nonprofits only

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pET28-his6-HA3-SNAPf

Plasmid

#188941

Purpose

T7 expression vector for making his6- and HA3-tagged SNAP protein

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

his6-HA3-SNAPf

Expression

Bacterial

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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NFAT/AP-1 3x luciferase

Plasmid

#11783

Depositor

Anjana Rao

Article

Macian et al EMBO J. 2000 Sep 1. 19(17):4783-95.

Insert

NFAT/AP-1 3x (Il2 Mouse)

Use

Luciferase

Tags

luciferase

Expression

Bacterial

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pET GFP LIC cloning vector (2GFP-T)

Plasmid

#29716

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, SiriusGFP, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pK19pA-MN

Plasmid

#86973

Purpose

ChIC; genomic mapping of chromatin proteins

Depositor

Ulrich Laemmli

Article

Schmid et al Mol Cell. 2004 Oct 8;16(1):147-57.

Insert

protein_A fused to Staphylococcus aureus microccocal nuclease (MNase)

Expression

Bacterial

Available Since

March 17, 2017

Availability

Academic Institutions and Nonprofits only

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pDRESS_mTurquoise2_spatial-linker-P2A_mScarlet-I3

Plasmid

#189755

Purpose

Produces an mTurquoise-antiFRET-mScarlet-I3 fusion protein in bacteria and in mamallian cells it produces 1:1 unfused mTurquoise2 and mScarlet-I3 FPs for ratiometric analysis

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mTurquoise2_spatial-linker-P2A_mScarlet-I3

Tags

6xHis

Expression

Bacterial and Mammalian

Promoter

CMV & Rha

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pFA6a-HaloTag-KanMX6

Plasmid

#87029

Purpose

Tag S. pombe genes in C terminal with HaloTag

Depositor

Julien Berro

Insert

HaloTag

Expression

Bacterial and Yeast

Available Since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pJM220 (pUC18T-miniTn7T-gm-rhaSR-PrhaBAD)

Plasmid

#110559

Purpose

miniTn7 delivery plasmid with rhaSR-PrhaBAD inducible promoter

Depositor

Joanna Goldberg

Article

Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

Insert

rhaSR-PrhaBAD

Expression

Bacterial

Promoter

rhaSR-PrhaBAD

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pBEST-p15A-PLtetO1-tetR-T500

Plasmid

#45391

Depositor

Vincent Noireaux

Article

Shin et al J Biol Eng. 2010 Jun 24;4:8.

Insert

tet repressor

Expression

Bacterial

Promoter

PL-tetO1

Available Since

June 13, 2013

Availability

Academic Institutions and Nonprofits only

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repressilator (pZS1-lTlrLLtCL)

Plasmid

#26489

Depositor

Michael Elowitz

Article

Elowitz et al Nature. 2000 Jan 20. 403(6767):335-8.

Insert

LacI, tetR

Expression

Bacterial

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pBS130

Plasmid

#26290

Depositor

Tom Clandinin

Article

Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

Insert

phiC31 integrase (int Streptomyces phage phiC31)

Use

Drosophila transgenesis

Expression

Bacterial and Insect

Available Since

Feb. 2, 2011

Availability

Academic Institutions and Nonprofits only

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pET28C-mCherry-HNRNPA1LCD

Plasmid

#204405

Purpose

E. coli expression of HNRNPA1 low-complexity domain (186-320) with an N-terminal mCherry tag

Depositor

Samie Jaffrey

Article

Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

Insert

HNRNPA1 (186-320) (HNRNPA1 Human)

Tags

6x-His; mCherry

Expression

Bacterial

Promoter

T7

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST14-Cys-DogCatcher

Plasmid

#171929

Purpose

Express Cys-DogCatcher protein in bacterial cytoplasm. The cysteine enables convenient coupling to maleimide-dye or thiol-reactive resin.

Depositor

Mark Howarth

Article

Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

Insert

Cys-DogCatcher

Tags

His6 and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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