4,908 results

1. pHW-CIBN-mCerulean-MP-HRW-CRY2-VHH(GFP)

   Plasmid

   #126025

   Purpose

   express RFP- and mCer-tagged LARIAT for optogenetic clustering

   Depositor

   Eurico Morais-de-Sá

   Article

   Osswald et al Biomolecules. 2019 Feb 12;9(2). pii: bio

   Insert

   CIBN-mCerulean-MP; CRY2 PHR-VHH(GFP)
   

   Use

   Tags

   RFP

   Expression

   Insect

   Mutation

   WT

   Promoter

   Hsp70

   Available since

   Sept. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUASP dsRed Rab10 WT

   Plasmid

   #44828

   Depositor

   Matthew Scott

   Article

   Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

   Insert

   Rab10 (Rab10 Fly)
   

   Use

   Drosophila

   Tags

   dsRed

   Expression

   Insect

   Mutation

   Promoter

   Available since

   April 18, 2013

   Availability

   Academic Institutions and Nonprofits only

3. Pub-mCD8:GFP-T2A-DsRed:NLS-SV40

   Plasmid

   #130665

   Purpose

   Plasmid to test the efficiency of T2A multiplexing in Aedes aegypti

   Depositor

   Leslie Vosshall

   Article

   Matthews et al eLife. 2019 May 21;8. pii: 43963. doi: 1

   Insert

   AaegPUb-mCD8-GFP-T2A-dsRed-NLS-SV40
   

   Use

   Ae. aegypti expression

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Aedes aegypti poly-ubiquitin

   Available since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMTgR2

   Plasmid

   #107286

   Purpose

   Plasmid can be used for Drosophila S2 driven secretion of extracellular part of human interferon-_ receptor 2.

   Depositor

   Bohdan Schneider

   Article

   Mikulecky et al Acta Crystallogr D Struct Biol. 2016 Sep

   Insert

   Interferon gamma receptor 2 (IFNGR2 Human)
   

   Use

   Tags

   CATCATCACCATCACCATGA

   Expression

   Insect

   Mutation

   Promoter

   Metallothionein promoter

   Available since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFastBac His HA GNU WT

   Plasmid

   #112994

   Purpose

   Baculovirus expression of His/HA-tagged Drosophila gnu

   Depositor

   Terry Orr-Weaver

   Article

   Hara et al Elife. 2017 May 30;6. doi: 10.7554/eLife

   Insert

   gnu (gnu Fly)
   

   Use

   Tags

   His/HA

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMCh-56

   Plasmid

   #92260

   Purpose

   Plasmid for expression of HA-tagged Drosophila melanogaster GW182 in D. melanogaster cells

   Depositor

   Marina Chekulaeva, Roy Parker

   Article

   Chekulaeva et al RNA. 2009 May;15(5):794-803. doi: 10.126

   Insert

   dGW182 (gw Fly)
   

   Use

   Tags

   HA

   Expression

   Insect

   Mutation

   Promoter

   Ac5

   Available since

   Dec. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pUAST myc mouse Rab35 CA

   Plasmid

   #53516

   Depositor

   Matthew Scott

   Article

   Zhang et al Science. 2009 Sep 4;325(5945):1250-4. do

   Insert

   Rab35 (Rab35 Mouse)
   

   Use

   Tags

   myc

   Expression

   Insect

   Mutation

   Q67L

   Promoter

   GAL4

   Available since

   June 4, 2014

   Availability

   Academic Institutions and Nonprofits only

8. EKAR2G_design1_mTFP_105_Venus_229

   Plasmid

   #39822

   Depositor

   Olivier Pertz

   Article

   Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

   Insert

   Erk activity reporter
   

   Use

   Tags

   His-Myc

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   CMV

   Available since

   Oct. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

9. LexAop2-myr::4xCLIPf

   Plasmid

   #87636

   Purpose

   For creating drosophila transgenics expressing LexAop2-myr4x::CLIPf

   Depositor

   Gregory Jefferis

   Article

   Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

   Insert

   myr::4xCLIPf
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pENTR-GFP-Patronin CC 638–1457

    Plasmid

    #59051

    Purpose

    for baculovirus expression of Patronin CC truncation (using Baculodirect kit from Invitrogen)

    Depositor

    Ron Vale

    Article

    Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

    Insert

    Patronin (Patronin Fly)
    

    Use

    Tags

    eGFP

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    Available since

    Sept. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pENTR-GFP-Patronin CC 776–1457

    Plasmid

    #59052

    Purpose

    for baculovirus expression of Patronin CC truncation (using Baculodirect kit from Invitrogen)

    Depositor

    Ron Vale

    Article

    Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

    Insert

    Patronin (Patronin Fly)
    

    Use

    Tags

    eGFP

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    Available since

    Sept. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

12. BacPAK HF-Uch37

    Plasmid

    #19425

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Yao et al Nat Cell Biol. 2006 Sep . 8(9):994-1002.

    Insert

    Uch37 (UCHL5 Human)
    

    Use

    Tags

    Flag and His

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

13. pUASp YFP Rab7 CA

    Plasmid

    #37704

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab7 (Rab7 Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Mutation

    Q67L (constitutive active)

    Promoter

    GAL4

    Available since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only

14. GRM1

    Plasmid

    #25353

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    GRM1-C254S:L28-G518 (GRM1 Human)
    

    Use

    Tags

    His

    Expression

    Insect

    Mutation

    C254S. Contains amino acids L28-G518.

    Promoter

    Available since

    July 29, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

15. CJ29 (let-7 perfect site)

    Plasmid

    #17766

    Depositor

    David Bartel

    Article

    Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

    Insert

    let-7 perfect site (let-7 Fly)
    

    Use

    Luciferase; Drosophila metallothionein gene promo…

    Tags

    renilla luciferase

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 15, 2008

    Availability

    Academic Institutions and Nonprofits only

16. pUC 3GLA UAS HAi

    Plasmid

    #71763

    Purpose

    gap-repair recombineering for Drosophila transgenesis, contains loxP cassette with GFP, Amp resistant, with 5xUAS and HA tag

    Depositor

    Matthias Soller

    Article

    Haussmann et al Nucleic Acids Res. 2018 Dec 12. pii: 523

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Dec. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOCC177

    Plasmid

    #118886

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and C-terminal SNAP tag, cleavable with TEV

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-MBP-3C-NotI-ccdB-AscI-TEV-SNAP-stop-HindIII cassette
    

    Use

    Tags

    HIS6-MBP, cleavable with 3C protease and SNAP tag…

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pOCC194

    Plasmid

    #118882

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal SNAP tag, cleavable with TEV, and C-terminal HIS6 tag, cleavable with 3C

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-NotI-ccdB-AscI-TEV-SNAP-3C-HIS6-stop-HindIII cassette
    

    Use

    Tags

    SNAP tag, cleavable with TEV protease, HIS6, clea…

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pOCC230

    Plasmid

    #118871

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal mCitrine, cleavable with TEV

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C--NotI-ccdB-AscI-TEV-mCitrine-stop-HindIII cassette
    

    Use

    Tags

    HIS6, cleavable with 3C protease and mCitrine, cl…

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pOCC69

    Plasmid

    #118874

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal monomeric GFP, cleavable with TEV

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C--NotI-ccdB-AscI-TEV-mGFP-stop-HindIII cassette
    

    Use

    Tags

    HIS6, cleavable with 3C protease and mGFP, cleava…

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge