4,908 results
-
Plasmid#83565PurposeExpresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the pseudosubstrate of PKCalpha.DepositorInsertPKC alpha (PRKCA Human)
UseTags10 nm ER/K Helix, FLAG Purificaiton Tag, Tev Prot…ExpressionBacterial and InsectMutationOnly catalytic domain of PKC (aa 335-672)PromoterT7 PromoterAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
PI(DM)-Rac1(WT)dc1.FLARE
Plasmid#87589PurposePI(DM): Photo-inhibitable/dark mutant C450A; dc1.FLARE: built based on GTPase FLARE dual chain sensorDepositorInsertsLOV2
PBD
Rac1
UseTagsYPet and dTurqExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
CJ24 (let-7 minigene)
Plasmid#17764DepositorInsertdme-let-7 minigene (let-7 Fly)
UseDrosophila metallothionein gene promoter drives e…TagsExpressionInsectMutationPromoterAvailable sinceApril 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
FoRC.PRm
Plasmid#84587PurposeDrosophila P-element transgenesis vector for cloning rescue transgenes. Cloning site is flanked by FRT sequences and contains constitutive RFP reporter.DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceDec. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUASP Flag Rab32 WT
Plasmid#44833DepositorInsertRab32 (Rab32 Fly)
UseDrosophilaTagsFlagExpressionInsectMutationPromoterAvailable sinceApril 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
hs casper RhoL-N17
Plasmid#17583DepositorInsertRhoL (RhoL Fly)
UseTagsExpressionInsectMutationN17PromoterAvailable sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMT-GFP-Spindly
Plasmid#24282DepositorInsertSpindly (Spindly Fly)
UseTagsGFPExpressionInsectMutationPromoterAvailable sinceJune 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMCh-139
Plasmid#92287PurposePlasmid for expression of NHA-tagged Drosophila melanogaster GW182 NED in D. melanogaster cellsDepositorInsertdGW182 NED (gw Fly)
UseTagsNHAExpressionInsectMutationdGW182 NED: aa 205-490PromoterAc5Available sinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUAST YFP Rab2 WT
Plasmid#58444Purposeexpression of drosophila YFP-Rab2DepositorInsertRab2 (Rab2 Fly)
UseTagsYFPExpressionInsectMutationPromoterGAL4Available sinceJuly 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSTARR-seq_fly-NipB
Plasmid#71502PurposeflySTARR-seq screening vector with NipB core promoterDepositorTypeEmpty backboneUseFlystarr-seq screening vectorTagssgGFP (SuperGlo, Qbiogene, Inc)ExpressionInsectMutationPromoterAvailable sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rab6 WT
Plasmid#37696DepositorInsertRab6 (Rab6 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationPromoterGAL4Available sinceNov. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pattB-aubergine-ADH-gf
Plasmid#69448Purposegenomic fragment of Drosophila aubergine was cloned from w1 background and inserted into pattB plasmid; mutagenesis PCR was used to change the catalytic residue DDH to ADH to express cat-dead AubDepositorInsertaubergine (aub Fly)
UseTagsNAExpressionInsectMutationchr2L:10,998,626(−)T>GPromoteraubergineAvailable sinceOct. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRmHa3-dKDM4A-HA2FL2
Plasmid#20232DepositorInsertdKDM4A (Kdm4A Fly)
UseTagsFLAG2 and HA2ExpressionInsectMutationPromoterAvailable sinceMarch 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
-
-
pUAST YFP Rab6 DN
Plasmid#58448Purposeexpression of drosophila YFP-Rab6 dominant negative mutantDepositorInsertRab6 (Rab6 Fly)
UseTagsYFPExpressionInsectMutationT26N (dominant negative)PromoterGAL4Available sinceJuly 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUAST YFP Rab6 WT
Plasmid#58447Purposeexpression of drosophila YFP-Rab6DepositorInsertRab6 (Rab6 Fly)
UseTagsYFPExpressionInsectMutationPromoterGAL4Available sinceJuly 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-hDcr-K70A.
Plasmid#89145PurposeExpression of helicase mutant of human Dicer in Sf9 cellsDepositorInserthDicer (DICER1 Human)
UseTagsHis6-tevExpressionInsectMutationK70APromoterAvailable sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
EKAR2G_design2_mTFP_227_Venus_173
Plasmid#40016DepositorInsertErk activity reporter
UseTagsHis-MycExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFasBac+GFP-CAMSAP3 CC
Plasmid#59042Purposefor baculovirus expression of mouse CAMSAP3 CC in Sf9 cellsDepositorInsertCAMSAP3 CC domain (Camsap3 Mouse)
UseTagsHis and eGFPExpressionInsectMutationPromoterAvailable sinceSept. 2, 2014AvailabilityAcademic Institutions and Nonprofits only