4,908 results

1. pFastBac1-NEDD4L_W2-W3-W4-HECT (349)

   Plasmid

   #187721

   Purpose

   Express GST-tagged NEDD4L W2-W3-W4-HECT (349) in insect cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L residues 349-975 (NEDD4L Human)
   

   Use

   Tags

   GST-HRV 3C site

   Expression

   Insect

   Mutation

   deletion of residues 1-348

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFastBacHTA-NEDD4L_FL

   Plasmid

   #187722

   Purpose

   Express His-tagged NEDD4L FL in insect cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L FL (NEDD4L Human)
   

   Use

   Tags

   His-TEV

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFastBacHTA-NEDD4L_C2-W1-L-W2-HECT

   Plasmid

   #187723

   Purpose

   Express His-tagged NEDD4L C2-W1-L-W2-HECT chimera in insect cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L residues 1-418+582-975 (NEDD4L Human)
   

   Use

   Tags

   His-TEV

   Expression

   Insect

   Mutation

   deletion of residues 419-581

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFastBacHTA-NEDD4L_W1-L-W2-HECT

   Plasmid

   #187724

   Purpose

   Express His-tagged NEDD4L W1-L-W2-HECT chimera in inserct cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L residues 153-418+582-975 (NEDD4L Human)
   

   Use

   Tags

   His-TEV

   Expression

   Insect

   Mutation

   deletion of residues 1-152 and 419-581

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Dm_λHA-FLuc100_CSC

   Plasmid

   #210592

   Purpose

   Expresses lambdaHA-optimal Firefly (CSC metric; Drosophila)

   Depositor

   Olivia Rissland

   Article

   Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

   Insert

   LambdaHA-optimal Firefly luciferase (Dm optimization, CSC metric)
   

   Use

   Tags

   lambda HA

   Expression

   Insect

   Mutation

   Promoter

   Ac5

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Dm_Opt_Renilla_CSC

   Plasmid

   #210593

   Purpose

   Expresses Optimal Renilla luciferase (CSC metric; Drosophila)

   Depositor

   Olivia Rissland

   Article

   Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

   Insert

   Optimal Renilla luciferase (Optimized for Drosophila, CSC metric)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Ac5

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Dm_λHA-FLuc0_CSC

   Plasmid

   #210589

   Purpose

   Expresses lambdaHA-nonoptimal Firefly (CSC metric; Drosophila)

   Depositor

   Olivia Rissland

   Article

   Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

   Insert

   LambdaHA-nonoptimal Firefly luciferase (Dm optimization, CSC metric)
   

   Use

   Luciferase

   Tags

   lambda HA

   Expression

   Insect

   Mutation

   Promoter

   Ac5

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFastBacI-KDAC7 CD

   Plasmid

   #224345

   Purpose

   Expresses human KDAC7 (HDAC7) catalytic domain in insect cells

   Depositor

   Terry Watt

   Article

   Toro et al Protein Expr Purif. 2018 Jan;141:1-6. do

   Insert

   KDAC7 (HDAC7 Human)
   

   Use

   Tags

   TEV-cleavable His6

   Expression

   Insect

   Mutation

   Only includes residues 521-942 (catalytic domain).

   Promoter

   Polyhedrin

   Available since

   Oct. 22, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pFastBacI-KDAC6 H216A

   Plasmid

   #224351

   Purpose

   Expresses human KDAC6 (HDAC6) H216A in insect cells

   Depositor

   Terry Watt

   Article

   Toro et al Biochemistry. 2023 May 2;62(9):1464-1483

   Insert

   KDAC6 (HDAC6 Human)
   

   Use

   Tags

   TEV-cleavable His6

   Expression

   Insect

   Mutation

   Histidine 216 mutated to alanine

   Promoter

   Polyhedrin

   Available since

   Oct. 22, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pFastBacI-KDAC6 H611A

    Plasmid

    #224352

    Purpose

    Expresses human KDAC6 (HDAC6) H611A in insect cells

    Depositor

    Terry Watt

    Article

    Toro et al Biochemistry. 2023 May 2;62(9):1464-1483

    Insert

    KDAC6 (HDAC6 Human)
    

    Use

    Tags

    TEV-cleavable His6

    Expression

    Insect

    Mutation

    Histidine 611 mutated to alanine

    Promoter

    Polyhedrin

    Available since

    Oct. 22, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pSN539

    Plasmid

    #226775

    Purpose

    Expresses Drosophila UNC-104(wild-type) in insect cells

    Depositor

    Kyoko Chiba

    Article

    Niwa et al J Cell Sci. 2024 Sep 6:jcs.262017. doi:

    Insert

    Drosophila unc-104 (unc-104 Fly)
    

    Use

    Tags

    sfGFP and 2xStrepII

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pKC51

    Plasmid

    #226779

    Purpose

    Expresses Drosophila UNC-104(A255V) in insect cells

    Depositor

    Kyoko Chiba

    Article

    Niwa et al J Cell Sci. 2024 Sep 6:jcs.262017. doi:

    Insert

    Drosophila unc-104 (A255V) (unc-104 Fly)
    

    Use

    Tags

    sfGFP and 2xStrepII

    Expression

    Insect

    Mutation

    changed Alanine 255 to Valine

    Promoter

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFB1.HMBP.PrS.CBX8ΔChromo

    Plasmid

    #224708

    Purpose

    Expresses a human CBX8 truncation (deletion of the chromodomain) in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag

    Depositor

    Chen Davidovich

    Article

    Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

    Insert

    CBX8 chromodomain truncation (CBX8 Human)
    

    Use

    Tags

    HMBP

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFBOH-MHL.mEGFP-CBX8

    Plasmid

    #224710

    Purpose

    Expresses N-terminal hexahistidine-tagged human CBX8 in insect cells, with a monomeric variant EGFP tag.

    Depositor

    Chen Davidovich

    Article

    Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

    Insert

    CBX8 (CBX8 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    Oct. 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pFB1.HMBP.PrS.CBX8-KR21A

    Plasmid

    #224709

    Purpose

    Expresses a human CBX8 mutant (KR21A) in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

    Depositor

    Chen Davidovich

    Article

    Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

    Insert

    CBX8 KR21A mt (CBX8 Human)
    

    Use

    Tags

    HMBP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFBOH-MHL.RING1B

    Plasmid

    #224712

    Purpose

    Expresses N-terminal hexahistidine-tagged human RING1B (RNF2) in insect cells.

    Depositor

    Chen Davidovich

    Article

    Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

    Insert

    RING1b (RNF2 Human)
    

    Use

    Tags

    6x histidine tag

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    Oct. 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pFB1.HMBP.PrS.CBX8

    Plasmid

    #224707

    Purpose

    Expresses human CBX8 in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

    Depositor

    Chen Davidovich

    Article

    Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

    Insert

    CBX8 (CBX8 Human)
    

    Use

    Tags

    HMBP

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFBOH-MHL.BMI1

    Plasmid

    #224711

    Purpose

    Expresses N-terminal hexahistidine-tagged human BMI1 (PCGF4) in insect cells.

    Depositor

    Chen Davidovich

    Article

    Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

    Insert

    BMI1 (BMI1 Human)
    

    Use

    Tags

    6x histidine tag

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFB-D3-ASK1

    Plasmid

    #226619

    Purpose

    Co-expression of D3 and ASK1 complex in insect cells

    Depositor

    Ning Zheng

    Article

    Shabek et al Nature. 2018 Nov;563(7733):652-656. doi:

    Inserts

    D3
    ASK1 (SKP1 Mustard Weed)
    

    Use

    Tags

    His tag followed with three copies of Msyb

    Expression

    Insect

    Mutation

    The flexible loop in D3 (Q478-D515) can be remove…

    Promoter

    Polyhedrin

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFastBac1-NpSxph

    Plasmid

    #193097

    Purpose

    Protein expression in insect cells

    Depositor

    Dan Minor

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

    Insert

    Nanorana parkeri saxiphilin
    

    Use

    Tags

    3C-eGFP-His10

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge