4,958 results

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pAc-sgRNA-Cas9.dora_3

Plasmid

#190702

Purpose

Expresses sgRNA targeting Dora gene and Cas9-Puro in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

dora (CG34401) sgRNA 3

Use

CRISPR

Expression

Insect

Promoter

Drosophila U6

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pBABR mCherry-Bcd-LEXY

Plasmid

#182596

Purpose

Drosophila integration plasmid expressing mCherry-Bcd-LEXY fusion protein under the maternal tubulin promoter.

Depositor

Jared Toettcher

Article

Singh et al Cell Rep. 2022 Mar 22;38(12):110543. doi

Insert

Bcd (bcd Fly)

Tags

LEXY and mCherry

Expression

Insect

Available Since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pBABR Bcd-LEXY

Plasmid

#182594

Purpose

Drosophila integration plasmid expressing Bcd-LEXY fusion protein under the maternal tubulin promoter.

Depositor

Jared Toettcher

Article

Singh et al Cell Rep. 2022 Mar 22;38(12):110543. doi

Insert

Bicoid (bcd Fly)

Tags

LEXY

Expression

Insect

Available Since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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IR25a-T2A-QF2

Plasmid

#162522

Purpose

Plasmid for CRISPR-mediated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment replacing the stop codon of the endogenous Aedes aegypti Ir25a gene

Depositor

Leslie Vosshall

Article

Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.

Insert

Ir25a-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Ir25a-right-HDR-arm

Expression

Insect

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pAC8-Mcl1_deltaTM

Plasmid

#187118

Purpose

insect cell expression of Mcl1-deltaTM

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

MCL-1 (BCL2 Human)

Tags

Strep

Expression

Insect

Mutation

delta328-350

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAC8-DDIT4_delta8

Plasmid

#187117

Purpose

insect cell expression of DDIT4-delta1-8

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

DDIT4 (DDIT4 Human)

Tags

Strep

Expression

Insect

Mutation

delta1-8

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAC8-HUWE1-HECTonly

Plasmid

#187119

Purpose

insect cell expression of HUWE1 HECT domain

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Tags

Strep

Expression

Insect

Mutation

delta1-3992

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAc-His-zfCsf1b

Plasmid

#168104

Purpose

Express soluble His-tagged zebrafish Csf1b in insect cells

Depositor

Petr Bartunek

Article

Hason et al Blood Adv. 2022 Jan 3. pii: 483305. doi:

Insert

Csf1b (csf1b Zebrafish)

Tags

6x His

Expression

Insect

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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Donor_C_FH_VIR

Plasmid

#186660

Purpose

Donor plasmid to endogenously tag VIR gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

OSS VIR C-terminal 3xFlag-3xHA Tag Donor Plasmid (vir Fly)

Tags

3xFlag-3xHA

Expression

Insect

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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GLE_sgRNA

Plasmid

#186658

Purpose

GLE sgRNA plasmid

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

Gle sgRNA Plasmid (CG14749 Fly)

Expression

Insect

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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Donor_N_FH_Shu

Plasmid

#186655

Purpose

Donor plasmid to endogenously tag Shu gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

OSS Shu N-terminal 3xFlag-3xHA Tag Donor Plasmid (shu Fly)

Tags

3xFlag-3xHA

Expression

Insect

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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piwi_sgRNA

Plasmid

#186653

Purpose

piwi sgRNA plasmid

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

Piwi sgRNA Plasmid (piwi Fly)

Expression

Insect

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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Donor_N_FH_Piwi

Plasmid

#186651

Purpose

Donor plasmid to endogenously tag piwi gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

OSS PIWI N-terminal 3xFlag-3xHA Tag Donor Plasmid (piwi Fly)

Tags

3xFlag-3xHA

Expression

Insect

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmPaip2-V5His6_Q

Plasmid

#147284

Purpose

Insect Expression of DmPaip2

Depositor

Elisa Izaurralde

Insert

DmPaip2

Expression

Insect

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmPaip1_G

Plasmid

#146353

Purpose

Insect Expression of DmPaip1

Depositor

Elisa Izaurralde

Insert

DmPaip1

Expression

Insect

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1A-DmDCP2-E361Q-dsRNAres-V5His6_J

Plasmid

#146689

Purpose

Insect Expression of DmDCP2-E361Q-dsRNAres

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

DmDCP2-E361Q-dsRNAres (DCP2 Fly)

Expression

Insect

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-HA-SBP-DmDCP1-dsRNAres_J

Plasmid

#146711

Purpose

Insect Expression of DmDCP1-dsRNAres

Depositor

Elisa Izaurralde

Article

Nishihara et al Nucleic Acids Res. 2013 Oct;41(18):8692-

Insert

DmDCP1-dsRNAres (Dcp1 Fly)

Expression

Insect

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pMT-NTAP-DmDCP1_K

Plasmid

#146735

Purpose

Insect Expression of DmDCP1

Depositor

Elisa Izaurralde

Insert

DmDCP1 (Dcp1 Fly)

Expression

Insect

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-HA-DmDCP1_1-327-dsRNAres_H

Plasmid

#146501

Purpose

Insect Expression of DmDCP1_1-327

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

DmDCP1_1-327 (Dcp1 Fly)

Expression

Insect

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-HA-DmDCP1-dsRNAres_H

Plasmid

#146502

Purpose

Insect Expression of DmDCP1

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

DmDCP1 (Dcp1 Fly)

Expression

Insect

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1A-DmDCP2-dsRNAres-V5His6_I

Plasmid

#146584

Purpose

Insect Expression of DmDCP2-dsRNAres

Depositor

Elisa Izaurralde

Article

Nishihara et al Nucleic Acids Res. 2013 Oct;41(18):8692-

Insert

DmDCP2-dsRNAres (DCP2 Fly)

Expression

Insect

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmDCP2-dsRNAres-V5His6_I

Plasmid

#146585

Purpose

Insect Expression of DmDCP2-dsRNAres

Depositor

Elisa Izaurralde

Article

Nishihara et al Nucleic Acids Res. 2013 Oct;41(18):8692-

Insert

DmDCP2-dsRNAres (DCP2 Fly)

Expression

Insect

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-myc-SBP-DmDCP2-dsRNAres_J

Plasmid

#146629

Purpose

Insect Expression of DmDCP2-dsRNAres

Depositor

Elisa Izaurralde

Insert

DmDCP2-dsRNAres (DCP2 Fly)

Expression

Insect

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-myc-SBP-DmDCP2-E361A-dsRNAres_J

Plasmid

#146630

Purpose

Insect Expression of DmDCP2-E361A-dsRNAres

Depositor

Elisa Izaurralde

Insert

DmDCP2-E361A-dsRNAres (DCP2 Fly)

Expression

Insect

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-myc-SBP-DmDCP2-E361Q-dsRNAres_J

Plasmid

#146631

Purpose

Insect Expression of DmDCP2-E361Q-dsRNAres

Depositor

Elisa Izaurralde

Insert

DmDCP2-E361Q-dsRNAres (DCP2 Fly)

Expression

Insect

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmDCP1-Y34A_P

Plasmid

#147214

Purpose

Insect Expression of DmDCP1-Y34A

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

DmDCP1-Y34A (Dcp1 Fly)

Expression

Insect

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-HA-DmDCP1-N41AS100A-dsRNAres_P

Plasmid

#147215

Purpose

Insect Expression of DmDCP1-N41AS100A-dsRNAres

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

DmDCP1-N41AS100A-dsRNAres (Dcp1 Fly)

Expression

Insect

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-HA-DmDCP1-Y34F-dsRNAres_P

Plasmid

#147217

Purpose

Insect Expression of DmDCP1-Y34F-dsRNAres

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

DmDCP1-Y34F-dsRNAres (Dcp1 Fly)

Expression

Insect

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-HA-DmDCP1-D114AR115A-dsRNAres_Q

Plasmid

#147265

Purpose

Insect Expression of DmDCP1-D114AR115A-dsRNAres

Depositor

Elisa Izaurralde

Insert

DmDCP1-D114AR115A-dsRNAres (Dcp1 Fly)

Expression

Insect

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-HA-DmDCP1-F36A-dsRNAres_Q

Plasmid

#147266

Purpose

Insect Expression of DmDCP1-F36A-dsRNAres

Depositor

Elisa Izaurralde

Insert

DmDCP1-F36A-dsRNAres (Dcp1 Fly)

Expression

Insect

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

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