4,908 results

1. OA-1067L

   Plasmid

   #200253

   Purpose

   pBac-U6-gRNA(doublesex+Intersex+bTublin)-3xp3-tdTomato

   Depositor

   Omar Akbari

   Article

   Li et al bioRxiv. 2023 Nov 15:2023.04.18.537404.

   Insert

   6 gRNAs targeting Doublesex, Intersex, bTublin
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pALuWBB

   Plasmid

   #183202

   Purpose

   dsRed marked transgene for Crispr co-conversion with w+ gRNA and drop in cloning sites for 2 more gRNA

   Depositor

   Mitch McVey

   Insert

   w+ gRNA and scaffold followed by 2 gRNA with BbsI and BsaI cloning sites
   

   Use

   CRISPR; Recombines at attp landing site in fly ge…

   Tags

   Expression

   Insect

   Mutation

   Promoter

   U6-3

   Available since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFastBac_Dual_GST-TEV-EGFP-TBK1ΔCTD (res 1-666)

   Plasmid

   #198072

   Purpose

   Used for the expression and purification of EGFP labelled TBK1 carrying a mutation for the removal of the CTD (SMC Internal No. 1874)

   Depositor

   Sascha Martens

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   TBK1 (TBK1 Human)
   

   Use

   Tags

   GST-TEV-EGFP

   Expression

   Insect

   Mutation

   TBK1ΔCTD (res 1-666)

   Promoter

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFastBac_Dual_GST-TEV-EGFP-TBK1-E696K

   Plasmid

   #198037

   Purpose

   Used for the expression and purification of EGFP labelled TBK1 carrying E696K mutation (SMC Internal No.: 1649)

   Depositor

   Sascha Martens

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   TBK1 (TBK1 Human)
   

   Use

   Tags

   GST-TEV-EGFP

   Expression

   Insect

   Mutation

   E696K

   Promoter

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFastBac-GST-TEV-mCherry-TBK1

   Plasmid

   #198033

   Purpose

   Used for the expression and purification of mCherry labelled TBK1 (SMC Internal No.: 1675)

   Depositor

   Sascha Martens

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   TBK1 (TBK1 Human)
   

   Use

   Tags

   GST-TEV and mCherry

   Expression

   Insect

   Mutation

   E696K

   Promoter

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. ptAD-seq-ubi63E-Gal4-DBD

   Plasmid

   #111930

   Purpose

   Vector to express tAD candidates from a library cloned from fragmented transcription factor coding sequences fused to the Gal4 DNA binding domain.

   Depositor

   Alexander Stark

   Article

   Arnold et al EMBO J. 2018 Aug 15;37(16). pii: embj.20

   Insert

   tAD-seq Gal4-DBD
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   April 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGL4.26_4xUAS_DSCP_GFP

   Plasmid

   #111931

   Purpose

   Vector to express GFP (under the control of the DSCP and an array of 4 UAS sites)

   Depositor

   Alexander Stark

   Article

   Arnold et al EMBO J. 2018 Aug 15;37(16). pii: embj.20

   Insert

   4xUAS GFP
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   April 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAc5.1B-lambdaN-HA-DmGIGYF_1-640_AH

   Plasmid

   #148933

   Purpose

   Insect Expression of DmGIGYF1_1-640

   Depositor

   Elisa Izaurralde

   Article

   Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

   Insert

   DmGIGYF1_1-640 (Gyf Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAc5.1B-lambdaN-HA-DmGIGYF_331-374-GST-V5His6_AH

   Plasmid

   #148934

   Purpose

   Insect Expression of DmGIGYF_331-374

   Depositor

   Elisa Izaurralde

   Article

   Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

   Insert

   DmGIGYF_331-374 (Gyf Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAc5.1B-lambdaN-HA-DmGIGYF_641-1574_AH

    Plasmid

    #148936

    Purpose

    Insect Expression of DmGIGYF1_641-1574

    Depositor

    Elisa Izaurralde

    Article

    Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

    Insert

    DmGIGYF1_641-1574 (Gyf Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one silent mutation compared to the sequence give…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAc5.1B-lambdaN-HA-DmGIGYF-F361AF367A_AH

    Plasmid

    #148937

    Purpose

    Insect Expression of DmGIGYF-F361AF367A

    Depositor

    Elisa Izaurralde

    Article

    Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

    Insert

    DmGIGYF-F361AF367A (Gyf Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one silent mutation compared to the sequence give…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAc5.1B-lambdaN-HA-DmGIGYF-W349A_AH

    Plasmid

    #148938

    Purpose

    Insect Expression of DmGIGYF-W349A

    Depositor

    Elisa Izaurralde

    Article

    Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

    Insert

    DmGIGYF-W349A (Gyf Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one silent mutation compared to the sequence give…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAc5.1B-lambdaN-HA-DmGIGYF-WFFtoAAA_AH

    Plasmid

    #148939

    Purpose

    Insect Expression of DmGIGYF-WFFtoAAA

    Depositor

    Elisa Izaurralde

    Article

    Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

    Insert

    DmGIGYF-WFFtoAAA (Gyf Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one silent mutation compared to the sequence give…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAc5.1B-lambdaN-HA-DmGIGYF-Y58AY60AM65A_AH

    Plasmid

    #148940

    Purpose

    Insect Expression of DmGIGYF1-Y58AY60AM65A

    Depositor

    Elisa Izaurralde

    Article

    Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

    Insert

    DmGIGYF1-Y58AY60AM65A (Gyf Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAc5.1B-lambdaN-HA-DmGIGYF-YYMYFWFtoAAAAAAA_AH

    Plasmid

    #148941

    Purpose

    Insect Expression of DmGIGYF1-YYMFWFtoAAAAAAA

    Depositor

    Elisa Izaurralde

    Article

    Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

    Insert

    DmGIGYF1-YYMFWFtoAAAAAAA (Gyf Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one silent mutation compared to the sequence give…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAc5.1B-EGFP-DmHPat-PPFPPFtoAAAAAA_AH

    Plasmid

    #148908

    Purpose

    Insect Expression of DmHPat-PPFPPFtoAAAAAA

    Depositor

    Elisa Izaurralde

    Article

    Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

    Insert

    DmHPat-PPFPPFtoAAAAAA (Patr-1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    three silent mutations compared to the sequence g…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAc5.1B-lambdaN-HA-Dm4EHP-W114A_AH

    Plasmid

    #148909

    Purpose

    Insect Expression of Dm4EHP-W114A

    Depositor

    Elisa Izaurralde

    Article

    Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

    Insert

    Dm4EHP-W114A (4EHP Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    4 silent mutations compared to the sequence given…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAc5.1B-lambdaN-HA-DmGIGYF-C-del331-370-YYMYFWFtoAAAAAAA_AH

    Plasmid

    #148910

    Purpose

    Insect Expression of DmGIGYF-del331-370-YYMFWFtoAAAAAA

    Depositor

    Elisa Izaurralde

    Article

    Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

    Insert

    DmGIGYF-del331-370-YYMFWFtoAAAAAA (Gyf Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one silent mutation compared to the sequence give…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAc5.1B-lambdaN-HA-DmGIGYF-del331-370_AH

    Plasmid

    #148911

    Purpose

    Insect Expression of DmGIGYF-del331-370

    Depositor

    Elisa Izaurralde

    Article

    Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

    Insert

    DmGIGYF-del331-370 (Gyf Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one silent mutation compared to the sequence give…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAc5.1B-lambdaN-HA-DmGIGYF-del331-370-YFWFtoAAAA_AH

    Plasmid

    #148912

    Purpose

    Insect Expression of DmGIGYF-del331-370-YFWFtoAAAA

    Depositor

    Elisa Izaurralde

    Article

    Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

    Insert

    DmGIGYF-del331-370-YFWFtoAAAA (Gyf Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one silent mutation compared to the sequence give…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge