4,841 results

1. R989-X31-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61L

   Plasmid

   #159558

   Purpose

   Baculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61L

   Depositor

   Dominic Esposito

   Article

   Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

   Insert

   His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61L (KRAS Human)
   

   Use

   Tags

   His6-MBP-tev

   Expression

   Insect

   Mutation

   Q61L

   Promoter

   Available since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. Hy_pMT Flag-URH49 MUT

   Plasmid

   #110126

   Purpose

   Expresses Flag-tagged human URH49 (DDX39A) with 4 mutations that impact circRNA nuclear export activity

   Depositor

   Jeremy Wilusz

   Article

   Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

   Insert

   URH49 (DDX39A Human)
   

   Use

   Tags

   Flag

   Expression

   Insect

   Mutation

   4 amino acid mutations (RSFS motif changed to KSL…

   Promoter

   Metallothionein Promoter (pMT)

   Available since

   Sept. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. Hy_pMT Flag-Hel25E MUT

   Plasmid

   #110122

   Purpose

   Expresses Flag-tagged D. melanogaster Hel25E with 4 mutations that impact circRNA nuclear export activity

   Depositor

   Jeremy Wilusz

   Article

   Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

   Insert

   Hel25E (Hel25E Fly)
   

   Use

   Tags

   Flag

   Expression

   Insect

   Mutation

   4 mutations (KKLN motif changed to RSFS)

   Promoter

   Metallothionein Promoter (pMT)

   Available since

   Sept. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. OA-1050I (notch)

   Plasmid

   #132421

   Purpose

   express arrays of gRNA targeting Notch under dU6-3 promoter

   Depositor

   Omar Akbari

   Article

   Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

   Insert

   notch gRNA array
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   U6-3

   Available since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. OA-1050K (firefly luciferase)

   Plasmid

   #132422

   Purpose

   express arrays of gRNA targeting Firefly Luciferase under dU6-3 promoter

   Depositor

   Omar Akbari

   Article

   Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

   Insert

   firefly Luciferase gRNA array
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   U6-3

   Available since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. OA-1050U (cinnabar)

   Plasmid

   #132423

   Purpose

   express arrays of gRNA targeting Cinnabar under dU6-3 promoter

   Depositor

   Omar Akbari

   Article

   Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

   Insert

   cinnabar gRNA array
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   U6-3

   Available since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. OA-1050V (wingless)

   Plasmid

   #132424

   Purpose

   express arrays of gRNA targeting Wingless under dU6-3 promoter

   Depositor

   Omar Akbari

   Article

   Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

   Insert

   wingless gRNA array
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   U6-3

   Available since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. OA-1050Z4 (yellow)

   Plasmid

   #132425

   Purpose

   express arrays of gRNA targeting Yellow under dU6-3 promoter

   Depositor

   Omar Akbari

   Article

   Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

   Insert

   yellow gRNA array
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   U6-3

   Available since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. OA-1052B (Ubiq-Fluc_Ubiq-Rluc)

   Plasmid

   #132426

   Purpose

   Express Firefly Luciferase-eGFP and Renilla Luciferase under Ubiquitin-63E promoter

   Depositor

   Omar Akbari

   Article

   Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

   Inserts

   Ubiq promoter
   firefly luciferase
   T2A-eGFP
   Renilla Luciferase-SV40-P10
   reversed ubiq promoter
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. OA-1050R (Ubiq-dCasRx)

    Plasmid

    #132417

    Purpose

    Express dCasRx under Ubiquitin-63E promoter

    Depositor

    Omar Akbari

    Article

    Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

    Insert

    dCasRx
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Ubiq

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. OA-1050L (UASt-CasRx)

    Plasmid

    #132418

    Purpose

    Express CasRx under UASt promoter

    Depositor

    Omar Akbari

    Article

    Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

    Inserts

    CasRx
    UASt+hsp70
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    UASt

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. UAS-u(XXL)Cas9

    Plasmid

    #127387

    Purpose

    Expresses Cas9 at very low level

    Depositor

    Michael Boutros

    Article

    Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

    Insert

    uORF(XXL)-Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pKM33

    Plasmid

    #154167

    Purpose

    Aedes aegypti Rel2 (long isoform) with C-terminal IκB domain deleted, expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.

    Depositor

    Kevin Maringer

    Article

    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

    Insert

    Rel2 (long isoform)
    

    Use

    Tags

    FLAG

    Expression

    Insect

    Mutation

    C-terminal IκB domain deleted

    Promoter

    IE1 promoter and hr5 enhancer from Autographa cal…

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pKM5

    Plasmid

    #154166

    Purpose

    Aedes aegypti Rel2 (long isoform) expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.

    Depositor

    Kevin Maringer

    Article

    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

    Insert

    Rel2 (long isoform)
    

    Use

    Tags

    FLAG

    Expression

    Insect

    Mutation

    Promoter

    IE1 promoter and hr5 enhancer from Autographa cal…

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKM28

    Plasmid

    #154165

    Purpose

    Aedes aegypti Rel1A expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus

    Depositor

    Kevin Maringer

    Article

    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

    Insert

    Rel1A
    

    Use

    Tags

    FLAG

    Expression

    Insect

    Mutation

    Promoter

    IE1 promoter and hr5 enhancer from Autographa cal…

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Opie2-PuroR[dsxM]

    Plasmid

    #131616

    Purpose

    Expresses a functional PuroR only in males and dsRed in both genders.

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2020 Apr 30;11(1):2106. doi:

    Inserts

    PuroR[dsxM] (dsx Fly)
    dsRed
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Opie2 and hr5Ie1

    Available since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. Opie2-NeoR[TraF]

    Plasmid

    #131617

    Purpose

    Expresses a functional NeoR only in females and dsRed in both genders.

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2020 Apr 30;11(1):2106. doi:

    Insert

    NeoR[traF]
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Opie2

    Available since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pF3BGX-mTurquoise2

    Plasmid

    #138390

    Purpose

    RMCE vector for generating C-terminal mTurqouise2 fusion

    Depositor

    Shu Kondo

    Article

    Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

    Insert

    mTurquoise2
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKM634

    Plasmid

    #134219

    Purpose

    Baculovirus expression of CENP-HIKM

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

    Insert

    CENP HIKM
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

20. pDK580

    Plasmid

    #134205

    Purpose

    Baculovirus expresion of CENP-L and CENP-N

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

    Insert

    CENP-L and His-CENP-N
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pKM412

    Plasmid

    #134213

    Purpose

    Baculovirus expression of CENP-P and CENP-O

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

    Insert

    his-CENP-P + O
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pKM672

    Plasmid

    #134221

    Purpose

    Baculovirus expression of CENP-C

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

    Insert

    GST-CENP-C 1-509
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pKM692

    Plasmid

    #134224

    Purpose

    Baculovirus expression of CENP-C

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

    Insert

    GST-CENP-C 235-509
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pKM673

    Plasmid

    #134222

    Purpose

    Baculovirus expression of CENP-C

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

    Insert

    GST-CENP-C 510-end
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Myo 9 Avi+Flag

    Plasmid

    #134969

    Purpose

    Expression and purification of c.elegans Myo 9 (Hum 7) using baculovirus from pFastBac1

    Depositor

    Martin Bähler

    Article

    Liao et al J Biol Chem. 2010 Aug 6;285(32):24933-42

    Insert

    Myo 9 (hum-7 Nematode)
    

    Use

    Tags

    Avi and Flag

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    Jan. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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26. U6d-T

    Plasmid

    #117212

    Purpose

    Expresses gRNA in Aedes aegypti

    Depositor

    Omar Akbari

    Article

    Li et al Elife. 2020 Jan 21;9. pii: 51701. doi: 1

    Insert

    u6d promoter-gRNA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Insect

    Mutation

    Promoter

    U6

    Available since

    Jan. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pFB HTB_hMSH6_T1219D

    Plasmid

    #135466

    Purpose

    Expression human MSH6_T1219D mutant protein using baculovirus

    Depositor

    Peggy Hsieh

    Article

    Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

    Insert

    human MSH6_T1219D (MSH6 Human)
    

    Use

    Tags

    His-tag

    Expression

    Insect

    Mutation

    changed threonine to aspartic acid

    Promoter

    Available since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pFast-dCry-V5His

    Plasmid

    #136240

    Purpose

    Protein expression of D. melanogaster (Dm) cytochrome with C-terminal V5 and His tags

    Depositor

    Aziz Sancar

    Article

    Ozturk et al Biochemistry. 2009 Sep 15;48(36):8585-93

    Insert

    DmCry
    

    Use

    Tags

    His and V5

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. DYNLL2

    Plasmid

    #132531

    Purpose

    Human LC8-2, codon optimised for Sf9 expression.

    Depositor

    Andrew Carter

    Article

    Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

    Insert

    DYNLL2 (DYNLL2 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Codon optimised for Sf9 expression

    Promoter

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. DYNLT3

    Plasmid

    #132532

    Purpose

    Human TCTEX-3, codon optimised for Sf9 expression.

    Depositor

    Andrew Carter

    Article

    Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

    Insert

    DYNLT3 (DYNLT3 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Codon optimised for Sf9 expression

    Promoter

    Available since

    Oct. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge