4,908 results

1. pMT-FLAG-CBPΔ(N+HAT)

   Plasmid

   #182841

   Purpose

   An EcoRV fragment (encoding residues 1566-2142) was removed from pMT-FLAG-CBPΔN. Expression in S2 cells by CuSO4 induction.

   Depositor

   Peter Harte

   Article

   Tie et al Development. 2009 Sep;136(18):3131-41. d

   Insert

   CBP (w Fly)
   

   Use

   Tags

   FLAG tag

   Expression

   Insect

   Mutation

   dletion of residues 1566-2142 from CBP1476-3222

   Promoter

   MT

   Available since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMT-FLAG-CBPΔN

   Plasmid

   #182840

   Purpose

   A 5.2 kb Drosophila CBP cDNA fragment (cut by Nhe I and Nsi I), encoding C-terminal residues 1476-3222, was inserted into the pMT-FLAG vector. Expression in S2 cells by CuSO4 induction.

   Depositor

   Peter Harte

   Article

   Tie et al Development. 2009 Sep;136(18):3131-41. d

   Insert

   CBP cDNA fragment (cut by Nhe I and Nsi I) (nej Fly)
   

   Use

   Tags

   FLAG tag

   Expression

   Insect

   Mutation

   Promoter

   MT

   Available since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCasper-pNos-MCP-mCherry

   Plasmid

   #180275

   Purpose

   to epress MCP-mCherry fusion protein in the early fly embryos (driven by maternal nanos driver)

   Depositor

   Hernan Garcia

   Article

   Liu et al PLoS Comput Biol. 2021 May 18;17(5):e100

   Insert

   MCP-mCherry
   

   Use

   Tags

   mCherry

   Expression

   Insect

   Mutation

   Promoter

   nanos

   Available since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. p{3P3-mTurquoise::FRT,attB}

   Plasmid

   #182180

   Purpose

   fluorescent reporter gene with yeast recombination site within intron

   Depositor

   David Stern

   Article

   Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

   Insert

   mTurquoise
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   April 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. p{Mhc-F4-678-EGFP::GW,attB}

   Plasmid

   #182170

   Purpose

   fluorescent reporter gene with intron containing Gateway cloning cassette

   Depositor

   David Stern

   Article

   Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   April 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. p{3P3-mTurquoise::GW,attB}

   Plasmid

   #182169

   Purpose

   fluorescent reporter gene with intron containing Gateway cloning cassette

   Depositor

   David Stern

   Article

   Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

   Insert

   mTurquoise
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   April 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. 3xTRE DREaMR 3p3DsR

   Plasmid

   #182206

   Purpose

   3xTRE Tet-On cassette with 3xp3 DsRed maker for generating knockins with doxycycline-inducible rescue

   Depositor

   Craig Montell

   Article

   Chen et al Genetics. 2021 Nov 5;219(3). pii: 636806

   Insert

   rTetR-3XP3-DsRed
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   April 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. 1xTRE DREaMR 3p3DsR

   Plasmid

   #182204

   Purpose

   1xTRE Tet-On cassette with 3xp3 DsRed marker for generating knockins with doxycycline-inducible rescue.

   Depositor

   Craig Montell

   Article

   Chen et al Genetics. 2021 Nov 5;219(3). pii: 636806

   Insert

   rTetR-3XP3-DsRed
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   April 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAc5.1B-lambdaN-HA-DmDCP2-PB-V5His6_G

   Plasmid

   #146359

   Purpose

   Insect Expression of DmDCP2-PB

   Depositor

   Elisa Izaurralde

   Insert

   DmDCP2-PB (DCP2 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Contains an in-frame insertion that adds a Q afte…

   Promoter

   Available since

   April 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAc5.1B-lambdaN-HA-HsAGO2_E

    Plasmid

    #146224

    Purpose

    Insect Expression of HsAGO2

    Depositor

    Elisa Izaurralde

    Article

    Eulalio et al Nat Struct Mol Biol. 2008 Apr . 15(4):34

    Insert

    HsAGO2 (AGO2 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Contains a K660E mutation compared to the sequenc…

    Promoter

    Available since

    April 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAc5.1B-lambdaN-HA-HsAGO2-F2V2_F

    Plasmid

    #146286

    Purpose

    Insect Expression of HsAGO2-F2V2

    Depositor

    Elisa Izaurralde

    Article

    Eulalio et al Nat Struct Mol Biol. 2008 Apr . 15(4):34

    Insert

    HsAGO2-F2V2 (AGO2 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Contains F470V, F505V, K660E mutations compared t…

    Promoter

    Available since

    April 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAc5.1B-EGFP-DmHelz-E958Q_AH

    Plasmid

    #148884

    Purpose

    Insect Expression of DmHelz-E958Q

    Depositor

    Elisa Izaurralde

    Article

    Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

    Insert

    DmHelz-E958Q (CG9425 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    two silent an two non silent G1374S, R2076G mutat…

    Promoter

    Available since

    April 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pExpreS2-1-CR-PA

    Plasmid

    #175445

    Purpose

    HiFi-compatible destination vector for secreted overexpression of thrombin-cleavable, C-terminal Protein A tagged proteins with ExpreS2 Platform

    Depositor

    Wyatt Yue

    Article

    Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

    Type

    Empty backbone

    Use

    Tags

    BiP Secretion Peptide and Thrombin-Protein A

    Expression

    Insect

    Mutation

    Promoter

    Fused Actin-HSP70

    Available since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. BicC.Ca9.R

    Plasmid

    #168295

    Purpose

    Expresses Cas9 in Drosophila late germline cells

    Depositor

    Omar Akbari

    Article

    Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

    Insert

    BicC.Cas9-T2A-eGFP-p10
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. 1137B=Hsp70Bb-Cas9

    Plasmid

    #153284

    Purpose

    The attB plasmid harboring the Hsp70Bb-Cas9-T2A-eGFP-p10 and a mini-white marker.

    Depositor

    Omar Akbari

    Article

    Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

    Insert

    pHsp70Bb-SpCas9-T2A-eGFP-p10 (cas9 Synthetic)
    

    Use

    CRISPR

    Tags

    eGFP

    Expression

    Insect

    Mutation

    Promoter

    Dmel Hsp70Bb

    Available since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. 1046E=dgRNAs[traB,bTub]

    Plasmid

    #149425

    Purpose

    The attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel tra and bTub genes.

    Depositor

    Omar Akbari

    Article

    Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

    Insert

    U6.3-gRNAs[TraB, bTub]
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    U6.3

    Available since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. 1098E=TI-pgSIT[tra,bTub,Hasp70Bb-Cas9]

    Plasmid

    #149427

    Purpose

    The attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel tra and bTub genes, and Hsp70Bb-Cas9-T2A-eGFP-p10.

    Depositor

    Omar Akbari

    Article

    Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

    Inserts

    U6.3-gRNAs[TraB, bTub]
    Hsp70Bb-Cas9-T2A-eGFP-p10
    

    Use

    CRISPR

    Tags

    eGFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p{ie1-DsRed::FRT,attB}

    Plasmid

    #182190

    Purpose

    fluorescent reporter gene with yeast recombination site within intron

    Depositor

    David Stern

    Article

    Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

    Insert

    DsRed
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p{3P3-eGFP::GW,attB}

    Plasmid

    #182167

    Purpose

    fluorescent reporter gene with intron containing Gateway cloning cassette

    Depositor

    David Stern

    Article

    Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p{3P3-dsRed::GW,attB}

    Plasmid

    #182168

    Purpose

    fluorescent reporter gene with intron containing Gateway cloning cassette

    Depositor

    David Stern

    Article

    Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

    Insert

    DsRed
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge