Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

4,958 results

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pAc5.1B-lambdaN-HA-DmSmaug_763-999-V5His6_C

Plasmid

#146013

Purpose

Insect Expression of DmSmaug_763-999

Depositor

Elisa Izaurralde

Insert

DmSmaug_763-999 (smg Fly)

Expression

Insect

Mutation

2 silent mutations and a deletion of the AA 961-9…

Available Since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmTral-R42E_B

Plasmid

#145988

Purpose

Insect Expression of DmTral-R42E

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral-R42E (tral Fly)

Expression

Insect

Mutation

two silent mutations A198C, A387G and a mutation …

Available Since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmSmaug_1-762-V5His6_C

Plasmid

#146001

Purpose

Insect Expression of DmSmaug_1-762

Depositor

Elisa Izaurralde

Insert

DmSmaug_1-762 (smg Fly)

Tags

V5-6xHis

Expression

Insect

Mutation

NP_523987.1, 7 silent mutations compared to the s…

Available Since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmZuc_B

Plasmid

#145949

Purpose

Insect Expression of DmZuc

Depositor

Elisa Izaurralde

Insert

DmZuc (zuc Fly)

Expression

Insect

Available Since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmExu_B

Plasmid

#145939

Purpose

Insect Expression of DmExu

Depositor

Elisa Izaurralde

Insert

DmExu (exu Fly)

Expression

Insect

Available Since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmAGO1-F629V_A

Plasmid

#145898

Purpose

Insect Expression of DmAGO1-F629V

Depositor

Elisa Izaurralde

Article

Eulalio et al Nat Struct Mol Biol. 2008 Apr . 15(4):34

Insert

DmAGO1-F629V (AGO1 Fly)

Expression

Insect

Mutation

V315A, F629V, H913R - variation with 2 non silent…

Available Since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmLSm3_A

Plasmid

#145888

Purpose

Insect Expression of DmLSm3

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmLSm3 (LSm3 Fly)

Expression

Insect

Available Since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmDCP2_209-791_A

Plasmid

#145848

Purpose

Insect Expression of DmDCP2_209-791

Depositor

Elisa Izaurralde

Insert

DmDCP2_209-791 (DCP2 Fly)

Expression

Insect

Available Since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac1_GI.1_A37C-A44C

Plasmid

#162582

Purpose

Expresses norovirus GI.1 VP1 protein with mutations A37C-A44C in Sf9 insect cells

Depositor

Peter Kwong

Article

Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

Insert

VP1 protein from human norovirus GI.1

Expression

Insect

Mutation

Alanine 37 changed to Cysteine and Alanine 44 cha…

Promoter

polyhedring

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac1_GI.1_A37I-A44L

Plasmid

#162583

Purpose

Expresses norovirus GI.1 VP1 protein with mutations A37I-A44L in Sf9 insect cells

Depositor

Peter Kwong

Article

Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

Insert

VP1 protein from human norovirus GI.1

Expression

Insect

Mutation

A37I and A44L

Promoter

polyhedrin

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac1_GI.1_Q141V-P221L

Plasmid

#162584

Purpose

Expresses norovirus GI.1 VP1 protein with mutations Q141V-P221L in Sf9 insect cells

Depositor

Peter Kwong

Article

Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

Insert

VP1 protein from human norovirus GI.1

Expression

Insect

Mutation

Glutamine 141 changed to Valine and Proline 221 c…

Promoter

polyhedrin

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac1_GI.1_L144C-P221C

Plasmid

#162585

Purpose

Expresses norovirus GI.1 VP1 protein with mutations L144C-P221C in Sf9 insect cells

Depositor

Peter Kwong

Article

Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

Insert

VP1 protein from human norovirus GI.1

Expression

Insect

Mutation

Leucine 144 changed to Cysteine and Proline 221 c…

Promoter

polyhderin

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac1_GI.1_G131C-N172C

Plasmid

#162586

Purpose

Expresses norovirus GI.1 VP1 protein with mutations G131C-N172C in Sf9 insect cells

Depositor

Peter Kwong

Article

Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

Insert

VP1 protein from human norovirus GI.1

Expression

Insect

Mutation

Glycine 131 changed to Cysteine and Asparagine 17…

Promoter

polyhedrin

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac1_GI.1_N167C-L169C

Plasmid

#162587

Purpose

Expresses norovirus GI.1 VP1 protein with mutations N167C-L169C in Sf9 insect cells

Depositor

Peter Kwong

Article

Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

Insert

VP1 protein from human norovirus GI.1

Expression

Insect

Mutation

Asparagine 167 changed to Cysteine and Leucine 16…

Promoter

polyhedrin

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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1026L=U6-3-gRNA#1[Pol-γ35]

Plasmid

#159674

Purpose

Plasmid supports expression of gRNA targeting Pol-γ35 site #1, tagged with mini-White, and can be integrated with phC31.

Depositor

Omar Akbari

Article

Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

Insert

U6-3-gRNA#1[Pol-γ35]

Expression

Insect

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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1026M=U6-3-gRNA#2[Pol-γ35]

Plasmid

#159675

Purpose

Plasmid supports expression of gRNA targeting Pol-γ35 site #2, tagged with mini-White, and can be integrated with phC31.

Depositor

Omar Akbari

Article

Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

Insert

U6-3-gRNA#2[Pol-γ35]

Use

CRISPR

Expression

Insect

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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KIF13B

Plasmid

#163907

Purpose

Full length mouse KIF13B for purification from insect cells

Depositor

Martin Loose

Article

Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Insert

kif13b (Kif13b Mouse)

Tags

Cleavable 6 x HIS tag

Expression

Insect

Promoter

polyhedrin

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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KIF13B 1-584

Plasmid

#163908

Purpose

Motile fragment of mouse KIF13B (aa 1-584) for purification from insect cells.

Depositor

Martin Loose

Article

Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Insert

kif13b (Kif13b Mouse)

Tags

Cleavable 6 x HIS tag

Expression

Insect

Promoter

polyhedrin

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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mWasabi-ADAP1

Plasmid

#163906

Purpose

Full length mouse ADAP1 with N terminal fluorescent tag for purification from insect cells

Depositor

Martin Loose

Article

Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Insert

ADAP1 (Adap1 Mouse)

Tags

mWasabi fluorescent tag

Expression

Insect

Promoter

polyhedrin

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pUASTLOTattB_vhhGFP4::T48-Baz::mCherry

Plasmid

#163927

Purpose

Expression of GrabFP-A-extracellular under control of UAS or LOP enhancer

Depositor

Markus Affolter

Article

Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

Insert

Fusion of vhhGPF4 (extracellular) nanobody with T48 protein, Bazooka minimal localization sequence and mCherry fluorophor

Use

Cre/Lox

Tags

mCherry

Expression

Insect

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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