5,046 results

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908E

Plasmid

#160238

Purpose

TtgO(2x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3’UTR-Ubiquitin-Renilla-attB

Depositor

Omar Akbari

Article

Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

Inserts

Ttg Operator (2x)
hsp70 minimal promoter
p-element promoter
Firefly luciferase
Renilla luciferase
Ubiquitin promoter

Tags

T2A GFP

Expression

Insect

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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908G

Plasmid

#160239

Purpose

PipO(3x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3’UTR-Ubiquitin-Renilla-attB

Depositor

Omar Akbari

Article

Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

Inserts

Pip Operator (3x)
hsp70 minimal promoter
p-element promoter
Firefly luciferase
Renilla luciferase
Ubiquitin promoter

Tags

T2A GFP

Expression

Insect

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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908J

Plasmid

#160242

Purpose

CymO(2x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3’UTR-Ubiquitin-Renilla-attB

Depositor

Omar Akbari

Article

Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

Inserts

Cym Operator (2x)
hsp70 minimal promoter
p-element promoter
Firefly luciferase
Renilla luciferase
Ubiquitin promoter

Tags

T2A GFP

Expression

Insect

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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1025

Plasmid

#160235

Purpose

FlightN-TetR(OFF)-VP16-Sv40-attB

Depositor

Omar Akbari

Article

Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

Inserts

Tetracycline repressor
Flightin promoter

Tags

VP16

Expression

Insect

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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907K

Plasmid

#160236

Purpose

FlightN-PipR-VP16-Sv40-attB

Depositor

Omar Akbari

Article

Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

Inserts

pristinamycin repressor
Flightin promoter

Tags

VP16

Expression

Insect

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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62 pLIB_N-MBP_C_FKBP

Plasmid

#160311

Purpose

N-term 3C cleavable MBP, C-term FKBP tag for insect cell expression

Depositor

John Weir

Article

Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

Type

Empty backbone

Tags

6xHis, FKBP, and MBP

Expression

Insect

Promoter

polyhydrin

Available Since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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61 pLIB_N-Strep_C_FKBP

Plasmid

#160310

Purpose

N-term 3C cleavable 2xStrepII, C-term FKBP tag for insect cell expression

Depositor

John Weir

Article

Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

Type

Empty backbone

Tags

2xStrepII and FKBP

Expression

Insect

Promoter

polyhydrin

Available Since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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60 pLIB_HIS_MYC

Plasmid

#160309

Purpose

N-term 3C cleavable His-Myc tag for insect cell expression

Depositor

John Weir

Article

Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

Type

Empty backbone

Tags

12xHis and 6xMyc

Expression

Insect

Promoter

polyhydrin

Available Since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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OA-1045C

Plasmid

#125005

Purpose

Expresses gRNAs targeting hid, eve, and wingless

Depositor

Omar Akbari

Article

Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

Insert

eve gRNA (eve Fly)

Expression

Insect

Promoter

U6:3

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pUC57-attB2-SA(2)-CRY2-mCherry

Plasmid

#160441

Purpose

To create Cry2-mCherry lines from MiMIC lines with inserts into coding introns in Phase 2.

Depositor

Nicholas Tolwinski

Article

Bunnag et al J Mol Biol. 2020 May 1;432(10):3159-3176

Insert

Cryptochrome 2 (CRY2 Mustard Weed)

Tags

mCherry

Expression

Insect

Available Since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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LIC4B-His-TEV-TAK1-TAB1

Plasmid

#154874

Purpose

Insect cell optimized TAK1-TAB1 construct used to solve structure and deposit PDB ID: 4O91

Depositor

Kenneth Westover

Article

Tan et al J Med Chem. 2015 Jan 8;58(1):183-96. doi

Insert

TAK1-TAB1 (TAB1 Human)

Tags

LIC TEV His

Expression

Insect

Mutation

Codon optimized

Promoter

polyhedrin

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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Hy_pMT Flag-Hel25E WT

Plasmid

#110123

Purpose

Expresses Flag-tagged D. melanogaster Hel25E

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

Hel25E (Hel25E Fly)

Tags

Flag

Expression

Insect

Promoter

Metallothionein Promoter (pMT)

Available Since

Oct. 4, 2020

Availability

Academic Institutions and Nonprofits only

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R989-X27-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) G12C

Plasmid

#159554

Purpose

Baculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) G12C

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-188) G12C (KRAS Human)

Tags

His6-MBP-tev

Expression

Insect

Mutation

G12C

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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R725-X09-635: His6-tev-Hs.SPRED1(13-125)

Plasmid

#159581

Purpose

Baculovirus protein expression of His6-tev-Hs.SPRED1(13-125)

Depositor

Dominic Esposito

Article

Yan et al Cell Rep. 2020 Jul 21;32(3):107909. doi:

Insert

His6-tev-Hs.SPRED1(13-125) (SPRED1 Human)

Tags

His6-tev

Expression

Insect

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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R989-X29-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) G12V

Plasmid

#159556

Purpose

Baculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) G12V

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-188) G12V (KRAS Human)

Tags

His6-MBP-tev

Expression

Insect

Mutation

G12V

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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R989-X31-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61L

Plasmid

#159558

Purpose

Baculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61L

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61L (KRAS Human)

Tags

His6-MBP-tev

Expression

Insect

Mutation

Q61L

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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Hy_pMT Flag-UAP56 MUT

Plasmid

#110124

Purpose

Expresses Flag-tagged human UAP56 (DDX39B) with 4 mutations that impact circRNA nuclear export activity

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

UAP56 (DDX39B Human)

Tags

Flag

Expression

Insect

Mutation

4 mutations (KSLN motif changed to RSFS)

Promoter

Metallothionein Promoter (pMT)

Available Since

Sept. 27, 2020

Availability

Academic Institutions and Nonprofits only

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Hy_pMT Flag-Hel25E K91N

Plasmid

#110121

Purpose

Expresses Flag-tagged D. melanogaster Hel25E with K91N mutation that greatly reduces helicase activity

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

Hel25E (Hel25E Fly)

Tags

Flag

Expression

Insect

Mutation

K91N mutation

Promoter

Metallothionein Promoter (pMT)

Available Since

Sept. 27, 2020

Availability

Academic Institutions and Nonprofits only

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Hy_pMT Flag-Hel25E D193E

Plasmid

#110120

Purpose

Expresses Flag-tagged D. melanogaster Hel25E with D193E mutation that greatly reduces ATP binding affinity

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

Hel25E (Hel25E Fly)

Tags

Flag

Expression

Insect

Mutation

D193E mutation

Promoter

Metallothionein Promoter (pMT)

Available Since

Sept. 27, 2020

Availability

Academic Institutions and Nonprofits only

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OA-1050S (UASt-dCasRx)

Plasmid

#132419

Purpose

Express dCasRx under UASt promoter

Depositor

Omar Akbari

Article

Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

Inserts

CasRx
UASt+hsp70

Expression

Insect

Promoter

UASt

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pSBL_DK198

Plasmid

#156425

Purpose

Insect cell expression of 3a delta N(aa 42-275)-sfGFP, needs to be introduced into baculoviral bacmid

Depositor

Stephen Brohawn

Article

Kern et al bioRxiv [Preprint]. 2021 Jan 26:2020.06.

Insert

SARS-CoV-2 3a protein (aa 42-275)

Tags

sfGFP-His

Expression

Insect

Mutation

aa 42-275

Promoter

Polyhedrin

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pMT Draper-ITAM only-GFP

Plasmid

#126656

Purpose

Inducible expression of Draper-ITAM only-GFP mutant in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Draper with Cytoplasmic ITAM residues Tyr all others mutated to Phe

Expression

Insect

Mutation

Cytoplasmic ITAM residues Tyr all others mutated …

Available Since

June 18, 2020

Availability

Academic Institutions and Nonprofits only

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AlphaE

Plasmid

#124527

Purpose

pColE1_AlphaE

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial, Insect, and Mamm…

Available Since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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Opie2-PuroR[traF]

Plasmid

#131615

Purpose

Expresses a functional PuroR only in females and dsRed in both genders.

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2020 Apr 30;11(1):2106. doi:

Insert

PuroR[traF]

Expression

Insect

Promoter

Opie2

Available Since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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Opie2-NeoR[traF]+Opie2-PuroR[dsxM]

Plasmid

#131618

Purpose

Expresses a functional NeoR only in females, a functional PuroR only in males, and dsRed in both genders.

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2020 Apr 30;11(1):2106. doi:

Inserts

PuroR[dsxM]
NeoR[traF]

Expression

Insect

Promoter

Opie2

Available Since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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AT1G56120 X070_pECIA14

Plasmid

#114860

Purpose

Prey vector AT1G56120 X070_pECIA14 should be used with bait vector AT1G56120 X070_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G56120 (AT1G56120 Mustard Weed)

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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AT1G56120 X070_pECIA2

Plasmid

#115060

Purpose

Bait vector AT1G56120 X070_pECIA2 should be used with prey vector AT1G56120 X070_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G56120 (AT1G56120 Mustard Weed)

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pUASt-ssBiP-ESCargo

Plasmid

#140166

Purpose

Expresses a regulatable secretory cargo for Drosophila melanogaster

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

ssBip-APVNTT-DsRed-Express2-FKBP(LV)(C22V)

Expression

Insect

Promoter

hs70

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pUASt-ssBiP-ESCargo*

Plasmid

#140165

Purpose

Expresses a regulatable secretory cargo (without an ER export signal) for Drosophila melanogaster

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

ssBip-DsRed-Express2-FKBP(LV)(C22V)

Expression

Insect

Promoter

hs70

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pF3BGX-Venus

Plasmid

#138388

Purpose

RMCE vector for generating C-terminal Venus fusion

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

Venus

Expression

Insect

Available Since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

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