4,958 results
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Plasmid#190795PurposePRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements. Step 1 in cloning processDepositorInsertPRE-Hsp70Bb-Cas9-T2A-eGFP
UseCRISPRExpressionInsectAvailable SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
PALI1_PIR_pFB1
Plasmid#191249PurposeExpresses PALI1 from insect cellsDepositorInsertLCoR
TagsMBPExpressionInsectMutationNAPromoterPolyhedrinAvailable SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
MON +T111H
Plasmid#187432PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
ExpressionInsectMutationchanged threonine at 117 to histidinePromoterPH and p10Available SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr[K216E]
Plasmid#188600PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr[C399-625 CM]
Plasmid#188588PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertNibbler C-terminal domain, catalytic mutant
TagsFLAG-MycExpressionInsectMutationD435A [GAT=>GCC]; E435A [GAA=>GCC]Available SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr[C399-625]-Gwpep(Mm)
Plasmid#188589PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertGW-peptide (mouse) C-terminally tagged C-terminal domain of Nibbler
TagsFLAG-Myc and GWpep mmExpressionInsectAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr[K276A]
Plasmid#188597PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr[C399-625]-Gwpep(Dm)
Plasmid#188590PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertGW-peptide (drosophila) C-terminally tagged C-terminal domain of Nibbler
TagsFLAG-Myc and Gwpep dmExpressionInsectAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Gwpep(Dm)-Nbr[C399-625]
Plasmid#188592PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertGW-peptide (drosophila) N-terminally tagged C-terminal domain of Nibbler
TagsFLAG-Myc-Gwpep dmExpressionInsectAvailable SinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Gwpep(Mm)-Nbr[C399-625]
Plasmid#188591PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertGW-peptide (mouse) N-terminally tagged C-terminal domain of Nibbler
TagsFLAG-Myc-Gwpep mmExpressionInsectAvailable SinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr[R272A,K276A]
Plasmid#188596PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertNibbler (Nbr Fly)
TagsFLAG-MycExpressionInsectMutationR272A [CGG=> GCG], K276A [AAG=>GCG]Available SinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr[K284A]
Plasmid#188595PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorAvailable SinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr[R272A]
Plasmid#188594PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorAvailable SinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr[K187A]
Plasmid#188593PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorAvailable SinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr-CM
Plasmid#188585PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertNibbler Catalytic mutant
TagsFLAG-MycExpressionInsectMutationdeleted first ATG; D435A [GAT=>GCC]; E435A [GA…Available SinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr
Plasmid#188584PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorAvailable SinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr[N1-383]
Plasmid#188586PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertNibbler N-terminal domain
TagsFLAG-MycExpressionInsectMutationdeleted first ATGAvailable SinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr[C399-625]
Plasmid#188587PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertNibbler C-terminal domain
TagsFLAG-MycExpressionInsectAvailable SinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pACE-K2P4.1-StrepTagII
Plasmid#191474PurposeExpresses K2P4.1 with C-terminal StrepTagIIDepositorAvailable SinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
OA-1127B
Plasmid#190997PurposeExpresses arrays of gRNA targeting rel1 promoter under U6b/c promoterDepositorInsertU6b:sgRNArel1A1 - U6c:sgRNArel1A2 - U6b:sgRNArel1A3 - U6c:sgRNArel1A4
ExpressionInsectAvailable SinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
KEE
Plasmid#187419PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
ExpressionInsectPromoterPH and p10Available SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
p1X-UASTattB
Plasmid#189861PurposeFly cloning and transformationDepositorTypeEmpty backboneExpressionInsectAvailable SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-M13-6His-KIF1C_R169W-GFP
Plasmid#185979PurposeBaculovirus expression of human KIF1C with R169W mutation and 6xHis and GFP tags.DepositorInsertKIF1C (KIF1C Human)
Tags6xHis and eGFPExpressionInsectMutationR169W - mutation causing HSP (SPG58)Available SinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
TEG +H122D
Plasmid#187434PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
ExpressionInsectMutationchanged histidine at 128 to aspartic acidPromoterPH and p10Available SinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pACE-GIRK2-mC-S
Plasmid#172428PurposeExpression of full-length, human GIRK4 with C-terminal mCherry StrepTag IIDepositorAvailable SinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
FER +H111E
Plasmid#187429PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
ExpressionInsectMutationchanged histidine at 117 to glutamic acidPromoterPH and p10Available SinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
KEE +E111H
Plasmid#187431PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
ExpressionInsectMutationchanged glutamic acid at 117 to histidinePromoterPH and p10Available SinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
SNG +R111Q
Plasmid#187437PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
ExpressionInsectMutationchanged arginine at 117 to glutaminePromoterPH and p10Available SinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
FER +H122D
Plasmid#187428PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
ExpressionInsectMutationchanged histidine at 128 to aspartic acidPromoterPH and p10Available SinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
MON +N122H
Plasmid#187433PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
ExpressionInsectMutationchanged asparagine at 128 to histidinePromoterPH and p10Available SinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
OST +Q111R
Plasmid#187436PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
ExpressionInsectMutationchanged glutamine at 116 to argininePromoterPH and p10Available SinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
FER +H111T
Plasmid#187430PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
ExpressionInsectMutationchanged histidine at 117 to threoninePromoterPH and p10Available SinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
RAT +D122H
Plasmid#187426PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorExpressionInsectMutationchanged aspartic acid at 129 for histidinePromoterPH and p10Available SinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
RAT +R111E
Plasmid#187425PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorExpressionInsectMutationchanged arginine at 118 to glutamic acidPromoterPH and p10Available SinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
SNG
Plasmid#187424PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
ExpressionInsectPromoterPH and p10Available SinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
OST
Plasmid#187422PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
ExpressionInsectPromoterPH and p10Available SinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
CHI +N122D
Plasmid#187427PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
ExpressionInsectMutationchanged asparagine at 130 to aspartic acidPromoterPH and p10Available SinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
TEG
Plasmid#187421PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
ExpressionInsectPromoterPH and p10Available SinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
FER
Plasmid#187418PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
ExpressionInsectPromoterPH and p10Available SinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
CHI
Plasmid#187417PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
ExpressionInsectPromoterPH and p10Available SinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLIB_BSSHI_6xHisTEV-WIPI2d
Plasmid#190863PurposePlasmid for the expression of purification of WIPI2d: SMC Internal reference: SMC1216DepositorInsertWIPI2 (WIPI2 Human)
ExpressionBacterial and InsectMutationSNP C to G at position 4562 (Val)Available SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc-sgRNA-Cas9.scr_1
Plasmid#190703PurposeExpresses scrambled sgRNA and Cas9-Puro in Drosophila S2 cellsDepositorInsertScrambled sgRNA 1
UseCRISPRExpressionInsectPromoterDrosophila U6Available SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc-sgRNA-Cas9.scr_3
Plasmid#190705PurposeExpresses scrambled sgRNA and Cas9-Puro in Drosophila S2 cellsDepositorInsertScrambled sgRNA 3
UseCRISPRExpressionInsectPromoterDrosophila U6Available SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
HA-doraWT_rescue_construct
Plasmid#190608PurposePuromycin-selectable expression of HA-tagged Dora (CG34401) in Drosophila S2 cellsDepositorAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
dora[B]_rescue_construct
Plasmid#190637PurposePuromycin-selectable expression of Dora[T295M] (CG34401) in Drosophila S2 cellsDepositorInsertDorado (CG34401 Fly)
ExpressionInsectMutationChanged threonine 295 to methioninePromoterActin-5cAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
HA-dora[B]_rescue_construct
Plasmid#190638PurposePuromycin-selectable expression of HA-tagged Dora[T295M] (CG34401) in Drosophila S2 cellsDepositorInsertDorado (CG34401 Fly)
Tags3xHAExpressionInsectMutationChanged threonine 295 to methioninePromoterActin-5cAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
dora[A]_rescue_construct
Plasmid#190639PurposePuromycin-selectable expression of truncated Dora (CG34401) in Drosophila S2 cellsDepositorInsertDorado (CG34401 Fly)
ExpressionInsectMutationTruncated to contain amino acids 1-945PromoterActin-5cAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
HA-dora[A]_rescue_construct
Plasmid#190640PurposePuromycin-selectable expression of HA-tagged truncated Dora (CG34401) in Drosophila S2 cellsDepositorInsertDorado (CG34401 Fly)
Tags3xHAExpressionInsectMutationTruncated to contain amino acids 1-945PromoterActin-5cAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc-sgRNA-Cas9.dora_1
Plasmid#190700PurposeExpresses sgRNA targeting Dora gene and Cas9-Puro in Drosophila S2 cellsDepositorInsertdora (CG34401) sgRNA 1
UseCRISPRExpressionInsectPromoterDrosophila U6Available SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc-sgRNA-Cas9.dora_2
Plasmid#190701PurposeExpresses sgRNA targeting Dora gene and Cas9-Puro in Drosophila S2 cellsDepositorInsertdora (CG34401) sgRNA 2
UseCRISPRExpressionInsectPromoterDrosophila U6Available SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only