4,840 results

1. 1010Av2

   Plasmid

   #125642

   Purpose

   Expresses FAR protein under 10xUAS control

   Depositor

   Omar Akbari

   Article

   Parks et al PLoS Pathog. 2021 Oct 29;17(10):e1010027

   Insert

   FAR protein
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   10xUAS

   Available since

   April 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. 1010Bv2

   Plasmid

   #125643

   Purpose

   Expresses FAR protein under 10xUAS control

   Depositor

   Omar Akbari

   Article

   Parks et al PLoS Pathog. 2021 Oct 29;17(10):e1010027

   Insert

   FAR protein
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   10xUAS

   Available since

   April 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMT-Ex

   Plasmid

   #231106

   Purpose

   Inducible protein expression in insect cell culture. Stable cell line generation.

   Depositor

   Michael Jeltsch

   Type

   Empty backbone

   Use

   Tags

   6xHis and V5

   Expression

   Insect

   Mutation

   Promoter

   Drosophila metallothionein promoter, MT

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. TH1150-pOCC177-FUS(Nhe)-PLD_YtoF_RGG_RtoK

   Plasmid

   #221889

   Purpose

   Shuttle vector for baculovirus production, using FlashBac bacmid

   Depositor

   Anthony Hyman

   Article

   Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

   Insert

   FUS (FUS Human)
   

   Use

   Tags

   6xHis-MBP-PreScission and TEV-SNAP-tag

   Expression

   Insect

   Mutation

   In PLD all Tyr (Y) changed to Phe (F), in RGG (aa…

   Promoter

   PH promoter

   Available since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFastBac-Dual_BNIP3(1-158aa)-THROMBIN-GST (E44A L47A D49A A50K Q51A; deltaWIPI2)

   Plasmid

   #223777

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

   Insert

   BNIP3 soluble part (BNIP3 Human)
   

   Use

   Tags

   Thrombin cleavage-GST

   Expression

   Insect

   Mutation

   E44A L47A D49A A50K Q51A; disrupts WIPI2 binding

   Promoter

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFastBac-Dual_BNIP3(1-158aa)-THROMBIN-GST (W18A/L21A; deltaLIR)

   Plasmid

   #223778

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

   Insert

   BNIP3 soluble part (BNIP3 Human)
   

   Use

   Tags

   Thrombin cleavage-GST

   Expression

   Insect

   Mutation

   W18A/L21A; deletion of LIR

   Promoter

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFastBac-Dual_BNIP3(1-158aa)-THROMBIN-GST

   Plasmid

   #223764

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

   Insert

   BNIP3 soluble part (BNIP3 Human)
   

   Use

   Tags

   Thrombin cleavage-GST

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFastBac-Dual_BNIP3(1-158aa)-EGFP-TEV-GST

   Plasmid

   #223765

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

   Insert

   BNIP3 soluble part (BNIP3 Human)
   

   Use

   Tags

   EGFP-TEV-GST

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFastBac-Dual_BNIP3(1-158aa)-EGFP-TEV-GST (W18A/L21A; deltaLIR)

   Plasmid

   #223766

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

   Insert

   BNIP3 soluble part (BNIP3 Human)
   

   Use

   Tags

   EGFP-TEV-GST

   Expression

   Insect

   Mutation

   W18A/L21A; deletion of LIR

   Promoter

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBIG1a_GST-TEV-CK2alpha_CK2beta

    Plasmid

    #223740

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

    Insert

    CK2-alpha, CK2-beta (CSNK2A1 Human)
    

    Use

    Tags

    GST-TEV

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBIG1a_GST-TEV-CK2alpha'_CK2beta

    Plasmid

    #223741

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

    Insert

    CK2-alphaprime_CK2-beta (CSNK2A2 Human)
    

    Use

    Tags

    GST-TEV

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEW66

    Plasmid

    #232794

    Purpose

    COMPASS Fragment 1 (Cps60, Cps50, Cps35, Cps25, Cps15) in PBIG1a

    Depositor

    Cynthia Wolberger

    Article

    Worden et al Elife. 2020 Jan 10;9. pii: 53199. doi: 1

    Insert

    COMPASS Fragment 1 (Cps60, Cps50, Cps35, Cps25, Cps15) (SWD1, BRE2, SWD2, SDC1, SHG1 Budding Yeast)
    

    Use

    Tags

    No tags

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin promoter

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEW108

    Plasmid

    #232823

    Purpose

    Expresses yeast compass complex in insect cells

    Depositor

    Cynthia Wolberger

    Article

    Worden et al Elife. 2020 Jan 10;9. pii: 53199. doi: 1

    Insert

    COMPASS (Cps60, Cps50, Cps35, Cps25, Cps15, Flag-Set1, Cps30, Twinstrep-Cps40) (BRE2, SWD1, SET1, SPP1, SWD3, SWD2, SDC1, SHG1 Budding Yeast)
    

    Use

    Tags

    His6-3xFLAG, Twinstrep

    Expression

    Insect

    Mutation

    Set1(762-1080)

    Promoter

    polyhedrin promoter

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEW107

    Plasmid

    #232796

    Purpose

    COMPASS Fragment 2 (His-FLAG-ySet1, Cps30, Twinstrep-Cps40) in pBIG2ab

    Depositor

    Cynthia Wolberger

    Article

    Worden et al Elife. 2020 Jan 10;9. pii: 53199. doi: 1

    Insert

    COMPASS Fragment 2 (His-FLAG-ySet1, Cps30, Twinstrep-Cps40) (SET1, SPP1, SWD3 Budding Yeast)
    

    Use

    Tags

    His6-3xFLAG, Twinstrep

    Expression

    Insect

    Mutation

    ySet1(762-1080)

    Promoter

    polyhedrin promoter

    Available since

    March 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. 0A-1169

    Plasmid

    #220935

    Purpose

    A QUAS line that expresses VSR proteins in Ae. aegypti when crossed with a driver line, Opie-2 dsRed.

    Depositor

    Omar Akbari

    Insert

    Cricket paralysis virus (CrPV) 1A protein, Nodamura virus (NoV) B2 protein, and flock house virus (FHV) B2
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    hsp70

    Available since

    March 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFastBacI-KDAC8

    Plasmid

    #224343

    Purpose

    Expresses human KDAC8 (HDAC8) in insect cells

    Depositor

    Terry Watt

    Article

    Toro et al Protein Expr Purif. 2018 Jan;141:1-6. do

    Insert

    KDAC8 (HDAC8 Human)
    

    Use

    Tags

    TEV-cleavable His6

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    Feb. 26, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pKLBac-Bat-FluA-trimer

    Plasmid

    #229858

    Purpose

    Influenza A/little yellow-shouldered bat/Guatemala/060/2010(H17N10) polymerase heterotrimer (self-cleaving polyprotein)

    Depositor

    Stephen Cusack

    Article

    Pflug et al Nature. 2014 Dec 18;516(7531):355-60. do

    Inserts

    PA_Bat-FluA_AFC35437.1
    PB1_Bat-FluA_AFC35436
    PB2_Bat-FluA_AFC35435.1
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pZH61 pLIB-8His3C-spt4

    Plasmid

    #231649

    Purpose

    express S.cerevisiae spt4 in insect cells

    Depositor

    Jesper Svejstrup

    Article

    Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

    Insert

    spt4 (SPT4 Budding Yeast)
    

    Use

    Tags

    8His-HRV3C

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pZH60 pLIB-spt5

    Plasmid

    #231648

    Purpose

    express S.cerevisiae spt5 in insect cells

    Depositor

    Jesper Svejstrup

    Article

    Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

    Insert

    spt5 (SPT5 Budding Yeast)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHACK-Sparse.FRT10-GAL4.DBD

    Plasmid

    #232833

    Purpose

    Sparse.FRT10 GAL4.DBD for HACK system

    Depositor

    Liqun Luo

    Article

    Xu et al bioRxiv [Preprint]. 2024 Dec 4:2024.12.0

    Inserts

    FRT10-STOP-FRT10
    GAL4.DBD (GAL4 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pHACK-Sparse.FRT10-p65.AD

    Plasmid

    #232832

    Purpose

    Sparse.FRT10 p65.AD for HACK system

    Depositor

    Liqun Luo

    Article

    Xu et al bioRxiv [Preprint]. 2024 Dec 4:2024.12.0

    Inserts

    FRT10-STOP-FRT10
    p65.AD
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pHACK-Sparse.FRT10-GAL4

    Plasmid

    #232834

    Purpose

    Sparse.FRT10 GAL4 for HACK system

    Depositor

    Liqun Luo

    Article

    Xu et al bioRxiv [Preprint]. 2024 Dec 4:2024.12.0

    Inserts

    FRT10-STOP-FRT10
    GAL4 (GAL4 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. VT028327-FRT10-STOP-FRT10-p65.AD

    Plasmid

    #232827

    Purpose

    Enhancer VT028327 driven p65.AD, gated by Sparse.FRT10

    Depositor

    Liqun Luo

    Article

    Xu et al bioRxiv [Preprint]. 2024 Dec 4:2024.12.0

    Inserts

    FRT10-STOP-FRT10 (stop )
    p65.AD
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. VT028327-FRT10-STOP-FRT10-GAL4

    Plasmid

    #232829

    Purpose

    Enhancer VT028327 driven GAL4, gated by Sparse.FRT10

    Depositor

    Liqun Luo

    Article

    Xu et al bioRxiv [Preprint]. 2024 Dec 4:2024.12.0

    Inserts

    FRT10-STOP-FRT10
    GAL4 (GAL4 Budding Yeast)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBP-Sparse.FRT10-GAL4.DBD

    Plasmid

    #232831

    Purpose

    Enhancer-free Sparse.FRT10 GAL4.DBD with ccdB for Gateway

    Depositor

    Liqun Luo

    Article

    Xu et al bioRxiv [Preprint]. 2024 Dec 4:2024.12.0

    Inserts

    FRT10-STOP-FRT10
    GAL4.DBD (GAL4 Budding Yeast)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. act5C-mScarlet

    Plasmid

    #230906

    Purpose

    Ubiquitous expression of mScarlet in Drosophila melanogaster

    Depositor

    Michael Boutros

    Article

    Port et al bioRxiv 2024.11.26.625385

    Insert

    mScarlet
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    act5C

    Available since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. GMR11F02-Gal4 UAS-GFP

    Plasmid

    #230907

    Purpose

    Tissue-specific expression of GFP in wing and haltere imaginal discs.

    Depositor

    Michael Boutros

    Article

    Port et al bioRxiv 2024.11.26.625385

    Insert

    GFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    GMR11F02Gal4-UAS

    Available since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. UAST-FRT-GFP-FRT-uMCas12a+nls1x

    Plasmid

    #230908

    Purpose

    Double inducible expression of Cas12a+ with Gal4 and Flp.

    Depositor

    Michael Boutros

    Article

    Port et al bioRxiv 2024.11.26.625385

    Insert

    Cas12a+
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGRACE

    Plasmid

    #230910

    Purpose

    Tracing of CRISPR activity in whole animals.

    Depositor

    Michael Boutros

    Article

    Port et al bioRxiv 2024.11.26.625385

    Insert

    GFP
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAHW/Joseph2-Multitagged Plasmid

    Plasmid

    #226725

    Purpose

    Validates the expression and detection of epitope-tagged proteins of interest in Drosophila cells.

    Depositor

    Richard Cripps

    Article

    Robinson et al Biotechniques. 2024;76(7):299-309. doi:

    Insert

    pAHW/Joseph2
    

    Use

    Drosophila s2 cell expression

    Tags

    HA, Myc, GFP, Flag, V5, 6xHis

    Expression

    Insect

    Mutation

    Promoter

    Act5C

    Available since

    Jan. 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge